公开(公告)号：DE2546952A1

公开(公告)日：1976-04-29

申请号：DE2546952

申请日：1975-10-20

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N15/00 ,  G01N21/55 ,  G02B19/00 ,  G02B21/08 ,  G02B21/06

Abstract: An improved system is described for providing approximately equalized illumination at all points between first and second opposite portions of a total internally reflecting cell. The system comprises in addition to the cell, a source of radiation of at least one selected wavelength, means for directing the radiation along a plurality of paths, and means for introducing the radiation in at least two of the paths into first and second opposite portions of the cell. Means can be provided for varying the penetration angle of the radiation into the cell.

公开(公告)号：DE2459432A1

公开(公告)日：1975-07-03

申请号：DE2459432

申请日：1974-12-16

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/48 ,  C09B67/46 ,  G01N1/30 ,  G01N33/16

Abstract: A technique for dyeing biological samples with dyes that are relatively insoluble in water or with combinations of dyes which are incompatible. The technique involves forming an emulsion of an immiscible solvent as the dispersed phase in water. The solvent can contain a very high concentration of a dye which is relatively insoluble in water, therefore constituting a reservoir for the diffusion of dye from the high concentration dispersed phase, through the water to the staining sites. In another aspect of the invention, one or more dispersed phases of dyes in immiscible solvents suspended in water provides a dye system for using a plurality of dyes which normally one would avoid mixing because they are incompatible. Additionally, another dyestuff can be dissolved in the water phase of the emulsion.

公开(公告)号：DE2431203A1

公开(公告)日：1975-01-23

申请号：DE2431203

申请日：1974-06-28

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/48 ,  G01N15/02 ,  G01N21/64 ,  G01N33/16

Abstract: Free floating viruses are detected and sized by a method which combines fluorescent staining with the observation of a modulation of the fluorescence intensity by Brownian motion of the particles in combination with a spatial filter. Intensity modulation and fluorescence data provides a set of descriptors useful in distinguishing the type of virus involved, particularly if also combined with data regarding the scattering of light by the particles. The method and apparatus are also usable in connection with detection and classification of other biological particles having the same order of magnitude of size as viruses.

公开(公告)号：DE2152029C3

公开(公告)日：1981-09-24

申请号：DE2152029

申请日：1971-10-19

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS ,  MARGOSHES MARVIN ,  VOGENTHALER R

IPC: G01J3/28 ,  H04N3/30 ,  G01J1/42 ,  H04N5/30 ,  H04N7/18

公开(公告)号：CA1084731A

公开(公告)日：1980-09-02

申请号：CA246716

申请日：1976-02-27

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS ,  MCNAIR HAROLD M

IPC: G01N30/32 ,  G01N31/08

Abstract: A sensitive high resolution gas chromatography method for elution of volatile sample mixtures at controlled speed including intermittent stop flow operation. The method utilizes chromatographic exit column pressure above one atmosphere absolute up to and higher than 50 atmospheres absolute concurrently with controlled low velocity carrier gas movement within the column, The method facilitates stop flow operation for on line spectral analysis of eluted samples, and facilitates matching the speed of high resolution chromatograph operation to other on line analysis processes.

公开(公告)号：CA1052692A

公开(公告)日：1979-04-17

申请号：CA241980

申请日：1975-12-17

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/533 ,  G01N33/543 ,  G01N21/38

Abstract: Reagents for the detection of specific reactants, such as antigens in biological fluids, comprising a polymer coupled to an antibody, the polymer having a plurality of dye molecules coupled thereto. The reagent is prepared by covalently linking an end functional group of polymers such as polyethylene amine to an antibody, typically through an aldehyde linkage, the polymer molecule having had A plurality of dye molecules, coupled to the polymer through side reactive sites.

公开(公告)号：CH609777A5

公开(公告)日：1979-03-15

申请号：CH1558076

申请日：1976-12-10

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N21/64 ,  G01N21/52

Abstract: An arrangement containing a light source, a shutter (22) and a lens arrangement (24) is used to illuminate a fluorescent material to be studied, employing radiation of the fluorescence excitation wavelength of the material, the exposure being sufficient to bleach the material. The fluorescent emission resulting therefrom is measured by detector means (28, 30) which deliver an electrical signal which is proportional to the instantaneous intensity of the fluorescent emission. The time interval during which the fluorescent emission decays from the initial intensity to a defined fraction thereof is measured and said signal is integrated over the time interval. This method produces the greatest possible fluorescence signal, which makes it possible to study very small particles or those having a low quantum yield of the exciting radiation.

公开(公告)号：CH607022A5

公开(公告)日：1978-11-30

申请号：CH1016974

申请日：1974-07-23

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N33/536 ,  G01N33/533 ,  G01N21/52

公开(公告)号：AU496888B2

公开(公告)日：1978-11-09

申请号：AU7368374

申请日：1974-09-25

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N15/02 ,  G01N21/64 ,  G01N33/49 ,  G06F17/18 ,  G06M11/00 ,  G01N21/38 ,  G01N21/60

Abstract: 1481451 Measuring the mass and distribution of particles BLOCK ENG Inc 16 Sept 1974 [23 Oct 1973] 40233/74 Heading G1A In an apparatus for measuring the mass and distribution of particles, e.g. viruses, in a sample 20 that may be treated chemically so that they fluoresce, the light emanating from the particles after they have been subjected to radiation from a laser source 22 is received by a photo-multiplier 30. The light reaching the photo-multiplier passes through a spatial filter 38, e.g. a lire screen, microscope type viewing optics 24 and a filter 26 which is restricted to the fluorescent wavelengths. The detector's output is passed via a D.C. filter 32 and a R.M.S. converter 34 to provide steady state signals representative of "T", the average total D.C. signal in the detector output, and "R", the root mean square value of the oscillations in the detector output. These signals are then processed as shown to supply two display devices representing the statistical particle distribution N=T 2 /(R 2 -T), or the mass M of the dye bound to each particle M = (R 2 -T)/KT. An alternative similiar digital signal processing system Fig. 2 (not shown), is disclosed.

公开(公告)号：DE2656632A1

公开(公告)日：1978-06-15

申请号：DE2656632

申请日：1976-12-14

Applicant: BLOCK ENGINEERING

Inventor： HIRSCHFELD TOMAS

IPC: G01N21/64 ,  G01N21/52

Abstract: The material is irradiated with radiation of a wavelength to stimulate flourescence in the material and of a strength sufficient to bleach the material. Detectors pick up fluorescent emission produced during bleaching by the radiation and generate a signal with a parameter proportional to the instantaneous value of the intensity of fluorescent emission. The time interval required for the fluorescent emission to face to a predetermined fraction of its initial intensity is measured. The fluorescent emission signal is integrated over this time interval.