公开(公告)号：EP2185708B1

公开(公告)日：2016-05-04

申请号：EP08782639.2

申请日：2008-08-06

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  VAN DIEN, Stephen, J. ,  BURK, Mark, J.

IPC: C12N15/52 ,  C12P7/18 ,  C12N1/00

CPC classification number: C12P7/18 ,  B01D3/002 ,  C12N9/0006

Abstract: The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.

公开(公告)号：EP2137315B1

公开(公告)日：2014-09-03

申请号：EP08732315.0

申请日：2008-03-14

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  VAN DIEN, Stephen, J. ,  BURGARD, Anthony ,  NIU, Wei

IPC: C12P7/18

CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ±-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, ±-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or ±-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, ±-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

公开(公告)号：EP2334800A2

公开(公告)日：2011-06-22

申请号：EP09813563.5

申请日：2009-09-09

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12P7/16 ,  C12P1/04

CPC classification number: C12P7/18 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/12 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12Y101/01157 ,  C12Y402/01055

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：EP2462221B1

公开(公告)日：2017-02-22

申请号：EP10807185.3

申请日：2010-08-05

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

公开(公告)号：EP2756082A1

公开(公告)日：2014-07-23

申请号：EP12831648.6

申请日：2012-09-14

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E.

IPC: C12N15/74

CPC classification number: C12P5/026 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/1235 ,  C12N9/88 ,  C12N15/52 ,  C12P5/002 ,  C12Y402/03

Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.

公开(公告)号：EP2744906A1

公开(公告)日：2014-06-25

申请号：EP12826412.4

申请日：2012-08-17

Applicant: Genomatica, Inc.

Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J.

IPC: C12P7/18

CPC classification number: C12P7/16 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  Y02E50/10

Abstract: The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non- naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.

公开(公告)号：EP2566969A2

公开(公告)日：2013-03-13

申请号：EP11778232.6

申请日：2011-05-04

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  SUN, Jun ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12P5/02

CPC classification number: C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

Abstract translation: 本发明提供了具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体生产丁二烯的方法。

公开(公告)号：EP2137315A2

公开(公告)日：2009-12-30

申请号：EP08732315.0

申请日：2008-03-14

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  VAN DIEN, Stephen, J. ,  BURGARD, Anthony ,  NIU, Wei

IPC: C12P1/00

CPC classification number: C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

Abstract: The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or ±-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, ±-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or ±-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, ±-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

公开(公告)号：EP2427544B1

公开(公告)日：2019-07-17

申请号：EP10772921.2

申请日：2010-05-07

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10 ,  C12P13/00

公开(公告)号：EP2438036B1

公开(公告)日：2017-03-08

申请号：EP10784131.4

申请日：2010-06-03

Applicant: Genomatica, Inc.

Inventor： CLARK, Warren ,  JAPS, Michael ,  BURK, Mark, J.

IPC: C07C31/18 ,  C07C33/02 ,  C07C29/76 ,  C12P7/18

CPC classification number: C07C29/76 ,  C12P7/18 ,  C07C31/207

Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.