公开(公告)号：EP3507603A1

公开(公告)日：2019-07-10

申请号：EP17765041.3

申请日：2017-09-01

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： DESAI, Surbhi ,  NLEND, Marie ,  GEE, Kyle ,  BAKER, Matthew ,  AGGELER, Robert ,  SWEENEY, Scott ,  RUKAVISHNIKOV, Aleksey ,  HUANG, Shih-Jung

IPC: G01N33/58 ,  G01N33/533

公开(公告)号：EP3143400B1

公开(公告)日：2019-03-06

申请号：EP15727499.4

申请日：2015-05-15

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  KANG, Hee Chol ,  ZHOU, Wenjun ,  DUBBELS, Bradley ,  OLSZOWY, Michael ,  O'GRADY, Michael ,  HUANG, Shih-jung ,  MELQUIST, Penny

IPC: G01N33/50 ,  G01N33/58 ,  C09B11/24 ,  A61K49/00

公开(公告)号：EP2855596B1

公开(公告)日：2019-02-27

申请号：EP13714770.8

申请日：2013-03-14

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  RUKAVISHNIKOV, Aleksey ,  BEACHAM, Daniel ,  HUANG, Shih-Jung ,  JANES, Michael ,  ZHOU, Wenjun ,  BERLIER, Judith

IPC: A61K49/00 ,  C07D311/90 ,  G01N33/58 ,  G01N33/84 ,  G01N33/50 ,  C09B11/24

公开(公告)号：EP3215515A1

公开(公告)日：2017-09-13

申请号：EP15762807.4

申请日：2015-08-14

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  WANG, Xin ,  KANG, Hee, Chol ,  CHEN, Aimei ,  WU, Yexin ,  HU, Yi-Zhen ,  AGGELER, Robert ,  LOW, Quentin

IPC: C07F7/08 ,  C08G61/12 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58

CPC classification number: C08G61/123 ,  C07F7/0816 ,  C07F7/083 ,  C08G2261/122 ,  C08G2261/124 ,  C08G2261/1424 ,  C08G2261/145 ,  C08G2261/1644 ,  C08G2261/1646 ,  C08G2261/3142 ,  C08G2261/3244 ,  C08G2261/334 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/5222 ,  C08G2261/72 ,  C08G2261/94 ,  C08L65/00 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1491 ,  G01N33/533 ,  G01N33/545 ,  G01N33/582 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/0094

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

Abstract translation: 公开了含有一种或多种二苯并噻咯单体残基的水溶性共轭聚合物，以及制备和使用这种聚合物的组合物，试剂盒和方法。 还公开了用一个或多个水溶性基团取代的二苯并硅氧烷衍生物，以及制备和使用这些衍生物制备水溶性二苯并硅氧烷聚合物的方法。

公开(公告)号：EP3143400A1

公开(公告)日：2017-03-22

申请号：EP15727499.4

申请日：2015-05-15

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  KANG, Hee Chol ,  ZHOU, Wenjun ,  DUBBELS, Bradley ,  OLSZOWY, Michael ,  O'GRADY, Michael ,  HUANG, Shih-jung ,  MELQUIST, Penny

IPC: G01N33/50 ,  G01N33/58 ,  C09B44/04 ,  C09B11/22 ,  C09B11/28

CPC classification number: C09B11/24 ,  A61K49/0041 ,  G01N33/5005 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are rhodamine-based cell-tracking reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Abstract translation: 本文描述了用于短期和长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本文公开的化合物是基于罗丹明的细胞跟踪试剂，其在UV / VIS光谱的红色部分中发荧光，并提供明亮的荧光强度，均匀的细胞染色和在活细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：EP3143022A1

公开(公告)日：2017-03-22

申请号：EP15725202.4

申请日：2015-05-15

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  KANG, Hee Chol ,  ZHOU, Wenjun ,  DUBBELS, Bradley ,  OLSZOWY, Michael ,  O'GRADY, Michael

IPC: C07D471/04 ,  C07C251/24

CPC classification number: G01N33/5005 ,  C07C309/61 ,  C07C2603/24 ,  C07D471/04 ,  C07K16/2812 ,  C09B11/28 ,  G01N33/5014 ,  G01N33/5044

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

Abstract translation: 本文描述了荧光化合物和方法并且包含这些化合物。 本文公开的化合物是卡比洛啉试剂，其在UV / VIS光谱的红色部分发荧光并且提供明亮的荧光强度，均匀的细胞染色，以及在活细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：EP2928965A1

公开(公告)日：2015-10-14

申请号：EP13812349.2

申请日：2013-12-06

Applicant: Life Technologies Corporation

Inventor： DALLWIG, Jason ,  GEE, Kyle ,  AHNERT, Nancy ,  KANG, Hee Chol ,  YANG, Jongtae

IPC: C09B23/00 ,  C12Q1/68 ,  G01N33/52 ,  G01N33/58 ,  G01N33/68

CPC classification number: C12Q1/6825 ,  C09B23/00 ,  C09B23/04 ,  C09B23/06 ,  C09B23/083 ,  C09B23/086 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q2525/207 ,  C12Q2563/107 ,  C12Q2563/173

Abstract: Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of RNA compounds (e.g., miRNA).

Abstract translation: 本文提供了取代的不对称花青染料，包括用于检测RNA化合物（例如miRNA）的试剂盒，测定系统和方法。

公开(公告)号：EP2912459A2

公开(公告)日：2015-09-02

申请号：EP13786595.2

申请日：2013-10-25

Applicant: Life Technologies Corporation

Inventor： AGNEW, Brian ,  AGGELER, Robert ,  KANG, Hee Chol ,  CHEN, Aimei ,  GEE, Kyle

IPC: G01N33/50

CPC classification number: A61K51/10 ,  C07K14/435 ,  C07K16/18 ,  C12P21/005 ,  C12P21/06 ,  G01N33/534 ,  G01N33/582 ,  G01N33/6854

Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.

公开(公告)号：EP1991273B1

公开(公告)日：2013-07-03

申请号：EP07825657.5

申请日：2007-02-12

Applicant: Life Technologies Corporation

Inventor： AGNEW, Brian ,  GEE, Kyle ,  NYBERG, Tamara

IPC: C12P21/00 ,  C07K17/00

CPC classification number: C12P21/005 ,  A61K47/549 ,  A61K47/61 ,  C07K1/13 ,  C07K14/47 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12Y302/01096 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583 ,  Y10T436/17

Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.

公开(公告)号：EP2121718A2

公开(公告)日：2009-11-25

申请号：EP08729780.0

申请日：2008-02-13

Applicant: Life Technologies Corporation

Inventor： AGNEW, Brian ,  GEE, Kyle ,  KUMAL, Kapil ,  FORD, Maura

IPC: C07H21/00

CPC classification number: C12Q1/6806 ,  C07D207/40 ,  C07D263/32 ,  C07D295/22 ,  C07D307/58 ,  C07D311/88 ,  C09B11/24 ,  C12Q1/68 ,  C12Q1/6841 ,  C12Q1/686 ,  G01N33/5308 ,  G01N33/531 ,  G01N2333/91245 ,  Y10T436/143333

Abstract: Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.