Abstract:
A scanning photometer and attendant methods are provided. The scanning photometer is generally characterized by first and second fluorophore excitation sources, an objective lens, and a common emission detector for the detection of first and second fluorophore emission originating from the excitation of the fluorophores via passage of excitation energy, via an optical path of the objective lens, from the excitation sources. Excitation energy and emission energy conditioning elements are likewise provided, operatively interposed before or after the objective lens as the case may be.
Abstract:
An instrument for processing a biological sample includes a chassis. Connected to the chassis is a tape path along which a tape with a matrix of wells can be automatically advanced through the instrument, a dispensing assembly for dispensing the biological sample and a reagent into the matrix of wells of the tape to form a biological sample and reagent mixture, a sealing assembly for sealing the biological sample and reagent mixture in the tape, and an amplification and detection assembly for detecting a signal from the biological sample and reagent mixture in the matrix of wells in the tape.
Abstract:
A high throughput biological sample processing system includes a sample carrier with a plurality of wells that progresses through the high throughput biological sample processing system. The system further includes a sample dispensing module, a reagent dispensing module, an accumulation/incubation module, and a detection module. The detection module employs an optical measuring device to encapsulate a biological sample in one of the plurality of wells of the sample carrier and detect energy from the chemistry of the biological sample to determine the amount of an analyte in the biological sample.
Abstract:
A scanning photometer and attendant methods are provided. The scanning photometer is generally characterized by first and second fluorophore excitation sources, an objective lens, and a common emission detector for the detection of first and second fluorophore emission originating from the excitation of the fluorophores via passage of excitation energy, via an optical path of the objective lens, from the excitation sources. Excitation energy and emission energy conditioning elements are likewise provided, operatively interposed before or after the objective lens as the case may be.
Abstract:
A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel
Abstract:
A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.
Abstract:
Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.
Abstract:
An instrument for processing a biological sample includes a chassis. Connected to the chassis is a tape path along which a tape with a matrix of wells can be automatically advanced through the instrument, a dispensing assembly for dispensing the biological sample and a reagent into the matrix of wells of the tape to form a biological sample and reagent mixture, a sealing assembly for sealing the biological sample and reagent mixture in the tape, and an amplification and detection assembly for detecting a signal from the biological sample and reagent mixture in the matrix of wells in the tape.
Abstract:
A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.
Abstract:
A system for processing and analyzing a sample includes a belt with wells that proceeds through the system, a dispensing station that dispenses the sample and reagents into the wells of the belt, and a detection station that detects an analyte in the wells of the belt. The system further includes a wash and decontamination station for decontaminating the wells of the belt.