公开(公告)号：KR1020140064566A

公开(公告)日：2014-05-28

申请号：KR1020120132011

申请日：2012-11-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  안정현 ,  변미정 ,  김경운 ,  김영주 ,  김지훈 ,  안현승

IPC: C12N15/56 ,  C12N9/42 ,  C12N15/63 ,  A23K1/16

Abstract: The present invention relates to a fibrinogenase cel-KG55 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG55 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG55 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG55 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.

Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG55基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG55基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG55基因，通过提供纤维蛋白原酶cel-KG55基因和蛋白质产物，可以用于开发 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。

公开(公告)号：KR1020140064561A

公开(公告)日：2014-05-28

申请号：KR1020120132001

申请日：2012-11-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  안정현 ,  김경운 ,  변미정 ,  김영주 ,  김지훈 ,  안현승

IPC: C12N15/56 ,  C12N9/42 ,  C12N15/63 ,  A23K1/16

Abstract: The present invention relates to a fibrinogenase cel-KG37 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG37 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG37 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG37 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.

Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG37基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG37基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG37基因，通过提供纤维蛋白原酶cel-KG37基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。

公开(公告)号：KR1020140064535A

公开(公告)日：2014-05-28

申请号：KR1020120131957

申请日：2012-11-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  김경운 ,  변미정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈

IPC: C12N15/56 ,  C12N9/42 ,  C12N15/63 ,  A23K1/16

Abstract: The present invention relates to a fibrinogenase cel-KG11 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG11 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, by selecting a cel-KG11 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat and providing a fibrinogenase cel-KG11 gene and protein products therethrough, it can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.

Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG11基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶的cel-KG11基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，通过选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG11基因，通过其提供纤维蛋白原酶cel-KG11基因和蛋白质产物，可以用于开发低价位 饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。

公开(公告)号：KR1020130068003A

公开(公告)日：2013-06-25

申请号：KR1020110135109

申请日：2011-12-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  이현정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈 ,  변미정

IPC: C12N9/42 ,  C12N15/56 ,  C12N15/63 ,  C12P21/00

CPC classification number: C12N9/2437 ,  A23K10/14 ,  C07K16/40 ,  C11D3/38645 ,  C12N15/67 ,  C12P1/00 ,  Y02E50/10

Abstract: PURPOSE: A Cel3-KG22 product, cellulase, is provided to be used in a feed additive and a detergent composition by finding a cellulase gene from a ruminant stomach microorganism of a black goat, to develop biofuel. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. The host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing cellulase.

Abstract translation: 目的：通过从黑山羊的反刍动物胃微生物中发现纤维素酶基因来开发生物燃料，提供了Cel3-KG22产品纤维素酶用于饲料添加剂和洗涤剂组合物。 构成：源自反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 通过重组载体转化宿主细胞。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞并过度表达纤维素酶的步骤。

公开(公告)号：KR1020130068001A

公开(公告)日：2013-06-25

申请号：KR1020110135107

申请日：2011-12-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  이현정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈 ,  변미정

IPC: C12N9/42 ,  C12N15/56 ,  C12N15/63 ,  C12P21/00

CPC classification number: C12N9/2437 ,  A23K10/14 ,  C11D3/38645 ,  C12N15/67 ,  C12P1/00 ,  Y02E50/10

Abstract: PURPOSE: A protein of cel-KG19, cellulase, is provided to be used as a feed additive and to produce a detergent and biofuel. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase in the host cell comprises a step of tranducing the recombinant vector into the host cell and overexpressing cellulase.

Abstract translation: 目的：提供cel-KG19蛋白质纤维素酶，用作饲料添加剂，生产洗涤剂和生物燃料。 构成：来自黑山羊的反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量纤维素酶的方法包括将重组载体转染到宿主细胞中并过度表达纤维素酶的步骤。

公开(公告)号：KR100996967B1

公开(公告)日：2010-11-30

申请号：KR1020090002448

申请日：2009-01-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 김명희 ,  정완태 ,  김영춘 ,  안정현 ,  최기영 ,  조민식 ,  황보종 ,  박흥규

IPC: B01L3/14

Abstract: 본 발명은 각종 시험분석에 사용되는 시험관에 관한 것이다.
본 발명의 시험관은, 시험관 몸체의 높이방향 내주면 일측에, 하나 이상의 돌출부가 내주면 원주를 따라 링의 형상으로 형성된 구조를 가짐에 기술적 특징을 가지고 있다.
본 발명의 시험관은, 시험관에 담긴 분석대상용액을 증발시키거나 반응시키는 등의 경우, 용액에서 거품이 과다하게 발생하거나, 용액이 튀면서 내주면을 따라 상승하여 시험관 상부 내주면에 고형분이 부착 또는 용액이 외부로 유출되는 현상이 상기 돌출부에 의해 방지 또는 최소화됨으로써, 전처리 시간을 현저히 단축할 수 있는 장점이 있다.
시험관, 아미노산, 분석, 전처리

公开(公告)号：KR1020100083077A

公开(公告)日：2010-07-21

申请号：KR1020090002448

申请日：2009-01-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 김명희 ,  정완태 ,  김영춘 ,  안정현 ,  최기영 ,  조민식 ,  황보종 ,  박흥규

IPC: B01L3/14

Abstract: PURPOSE: A test tube is provided to reduce manufacturing costs, energy and water waste by reducing evaporation time with a protrusion part of a ring shape which is protruded according to the circumference of the inner circumference. CONSTITUTION: A test tube includes a protrusion part(E) formed according to the inner circumference on the inner circumference. The protrusion part is protruded from the inner circumference by a recess which is formed on the test tube. The protrusion part is a protruding member of a ring shape which is coupled on the inner circumference of the inner circumference. A height of the protrusion part is 10 ~ 20 % of the height of the test tube.

Abstract translation: 目的：提供试管，通过根据内圆周的周长突出的环形突起部分减少蒸发时间来降低制造成本，能量和水的浪费。 构成：试管包括根据内周的内周形成的突出部（E）。 突起部通过形成在试管上的凹部从内周突出。 突出部是联接在内周的内周上的环状的突出部件。 突出部的高度为试管高度的10〜20％。