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公开(公告)号：KR1020090122823A
公开(公告)日：2009-12-01
申请号：KR1020080048818
申请日：2008-05-26
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A root-specific expression promoter for expression of foreign gene in a plant is provided to massively produce the foreign gene through genetic engineering. CONSTITUTION: A root-specific promoter(PAtLPL1) comprises a gene sequence of the sequence number 1. The promoter has high similarity with LPL1 promoter of Arabidopsis thaliana. The LPL1 promoter region of the Arabidopsis thaliana contains 1,957bp upstream of gene coding region. A primer for amplifying the root-specific promoter comprises oligonucleotides of the sequence numbers 2 and 3. A transgenic plant cell is obtained by transforming with an expression vector pBGWFS7-PAtLPL1(KACC 95079P).
Abstract translation: 目的：提供用于在植物中表达外源基因的根特异性表达启动子，以通过基因工程大规模生产外源基因。 构成：根特异性启动子（PAtLPL1）包含序列号1的基因序列。启动子与拟南芥的LPL1启动子具有高相似性。 拟南芥的LPL1启动子区在基因编码区上游含有1,957bp。 用于扩增根特异性启动子的引物包含序列号2和3的寡核苷酸。通过用表达载体pBGWFS7-PAtLPL1（KACC 95079P）转化获得转基因植物细胞。
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公开(公告)号：KR1020090097714A
公开(公告)日：2009-09-16
申请号：KR1020080023031
申请日：2008-03-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A root-specific expression promoter which expresses an exogenous gene in a plant is provided to massively produce useful protein in transformed storage rood tissue. A root-specific promoter PAtM19PK comprises a sequence number 1 (SEQ ID NO:1). A primer for amplifying the promoter comprises an oligonecleotide of the sequence number 2 and an oligonucleotide of the sequence number 3. The root-specific promoter is a promoter which is related to the transcription of protein phosphorylation enzyme gene. The protein phosphorylation enzyme is related to interaction between protein-protein by having leucine-rich repeat motif.
Abstract translation: 提供在植物中表达外源基因的根特异性表达启动子，用于在转化的储存心脏组织中大量产生有用的蛋白质。 根特异性启动子PAtM19PK包含序列号1（SEQ ID NO：1）。 用于扩增启动子的引物包含序列号2的寡核苷酸和序列号3的寡核苷酸。根特异性启动子是与蛋白质磷酸化酶基因的转录相关的启动子。 蛋白磷酸化酶通过富含亮氨酸重复序列与蛋白质蛋白质之间的相互作用相关。
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公开(公告)号：KR1020080056499A
公开(公告)日：2008-06-23
申请号：KR1020060129479
申请日：2006-12-18
Applicant: 대한민국(농촌진흥청장)
Abstract: An aerial part specific promoter of a plant is provided to show excellent tissue specificity of inducing expression of a target gene specifically at an aerial part of the plant, thereby being usefully used to develop a transgenic plant capable of mass-producing high-value useful ingredients. An aerial part specific promoter of a plant includes a sequence of SEQ ID : NO. 1. A recombinant expression vector comprises the promoter and a sequence encoding a target protein operably linked to the promoter. A method for aerial part specific expression of a target protein comprises a step of introducing the recombinant expression vector into a plant, wherein the plant is dicotyledoneae or monocotyledoneae. Further, the aerial part is selected from a group consisting of leaf, stem, legume and flower.
Abstract translation: 提供植物的地上部分特异性启动子以显示在植物的地上部分特异性诱导靶基因表达的优异组织特异性，从而有用地开发能够大规模生产高价值有用成分的转基因植物 。 植物的地上部分特异性启动子包括SEQ ID NO： 重组表达载体包含启动子和编码与启动子可操作连接的靶蛋白的序列。 靶蛋白的地上部分特异性表达的方法包括将重组表达载体导入植物的步骤，其中植物是双子叶植物或单子叶植物。 此外，地面部分选自由叶，茎，豆类和花组成的组。
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Patent Agency Ranking
公开(公告)号：KR1020080018425A
公开(公告)日：2008-02-28
申请号：KR1020060080503
申请日：2006-08-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6851 , C12Q1/6844 , C12Q1/6895 , C12Q2537/143 , G01N33/5308
Abstract: A qualitative and quantitative detection method of genetically modified rice is provided to detect specifically the genetically modified rice in currently distributed or imported agricultural products and food by using PCR(polymerase chain reaction) primer and probe and standard plasmid, and select a homozygote line rice. A genetically modified rice is detected qualitatively and quantitatively by detecting a foreign gene introduced into the rice through PCR by using a PCR primer set CytC-F1265 of SEQ ID NO:1 and CytC-R1345 of SEQ ID NO:2, a probe CytC-1292 of SEQ ID NO:3 and a plasmid p0S2, or a PCR primer set GFP-F203 of SEQ ID NO:4 and GFP-R303 of SEQ ID NO:5, a probe GFP-232 of SEQ ID NO:6 and a plasmid p0S2.
Abstract translation: 提供转基因水稻的定性和定量检测方法，通过使用PCR（聚合酶链式反应）引物和探针和标准质粒来特异性检测目前分布或进口农产品和食品中的转基因水稻，并选择纯合水稻。 通过使用SEQ ID NO：1的PCR引物组CytC-F1265和SEQ ID NO：2的CytC-R1345，探针CytC-F1265，通过PCR检测引入水稻的外源基因，定性和定量检测转基因水稻， SEQ ID NO：3的1292和质粒pOS2，或SEQ ID NO：4的GFP引物组GFP-F203和SEQ ID NO：5的GFP-R303，SEQ ID NO：6的探针GFP-232和 质粒p0S2。
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公开(公告)号：KR100803391B1
公开(公告)日：2008-02-14
申请号：KR1020060086013
申请日：2006-09-07
Applicant: 대한민국(농촌진흥청장)
Abstract: A ubiquitous promoter is provided to be able to induce the systemic expression of Arabidopsis thaliana as well as other crops when modified by a plant genetic engineering method, thereby being used for expressing a useful gene, a marker or a reporter gene or improving agricultural characteristics. A ubiquitous promoter is isolated from Arabidopsis thaliana and consists of SEQ ID : NO. 1. A primer for amplifying the ubiquitous promoter consists of an oligonucleotide consisting of SEQ ID : NOs. 6 and 7. A transgenic plant is transformed by an expression vector including the ubiquitous promoter, where the plant is a root vegetable such as carrot, sweet potato and radish, a medicinal plant such as ginseng and Codonopis lanceolata, a forage crops such as corn, and staple grains such as rice.
Abstract translation: 提供普遍存在的启动子，以便通过植物基因工程方法进行修饰时能够诱导拟南芥以及其他作物的全身表达，从而用于表达有用的基因，标记或报告基因或改善农业特征。 普遍存在的启动子从拟南芥中分离并由SEQ ID NO： 用于扩增普遍存在的启动子的引物由由SEQ ID NO：1组成的寡核苷酸组成。 通过包括普遍存在的启动子的表达载体转化转基因植物，其中植物是根植物如胡萝卜，甘薯和萝卜，药用植物如人参和Codonopis lanceolata，饲料作物如玉米 ，和米等主食。
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公开(公告)号：KR100790809B1
公开(公告)日：2008-01-04
申请号：KR1020060049574
申请日：2006-06-01
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물체 내에 외래 유용유전자의 발현 부위를 뿌리로 제한하여 조절하는 뿌리 조직 특이 발현 유도 프로모터에 관한 것으로, 보다 상세하게는 애기장대에서 분리된 익스텐신 프로모터가 뿌리 특이적으로 발현됨으로써 뿌리 작물의 농업적 형질을 개량하거나 당근, 고구마, 인삼 뿌리 등을 이용한 유용 물질 생산시 식물 생육에 영향을 주지 않고 뿌리에 국한시키고자 하는 식물 형질전환체 개발시 유용한 AtLRX
프로모터 제공을 목적으로 한다.
익스텐신, 뿌리 특이 프로모터, 녹색형광 단백질, 청색발색 단백질-
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58.发明公开
公开(公告)号：KR1020040079053A
公开(公告)日：2004-09-14
申请号：KR1020030013955
申请日：2003-03-06
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Primer sets and probes for detection of GMO, and a standard plasmid for qualitative and quantitative detection of GMO are provided, thereby effectively carrying out qualitative and quantitative detection of GMO in agricultural products and foods. CONSTITUTION: The insect tolerant primer set for detection of GMO(genetically modified organism) plants comprises one or two primers selected from NLL-F primer of SEQ ID NO:1, NLL-R primer of SEQ ID NO:2, Cry3A-F primer of SEQ ID NO:6, Cry3A-R primer of SEQ ID NO:7 and Cry3A-F2 primer of SEQ ID NO:8. The Cyr3A-pb probe of SEQ ID NO:9 is used together with the insect tolerant primer set for detection of GMO plant. The UGP-pb probe of SEQ ID NO:14 is used together with the insect tolerant primer set for detection of GMO plant. The insect and virus tolerant primer set for detection of GMO plant such as a potato comprises one or two primers selected from NLYL-F primer of SEQ ID NO:3, NLYM-R primer of SEQ ID NO:4 and NLPS-R primer of SEQ ID NO:5. The primer set for detection of UDP-glucose phosphorylase gene of natural or GMO plants comprises one or two primers selected from UGP-F primer of SEQ ID NO:10, UGP-R primer of SEQ ID NO:11, UGP-F2 primer of SEQ ID NO:12 and UGP-R2 primer of SEQ ID NO:13. The standard plasmid pCryugp is used for qualitative and quantitative detection of GMO plants.
Abstract translation: 目的：提供用于检测转基因的引物组和探针，并提供用于定性和定量检测转基因的标准质粒，从而有效地进行农产品和食品中转基因生物的定性和定量检测。 构成：用于检测转基因生物（遗传修饰生物）植物的耐虫引物组包含选自SEQ ID NO：1的NLL-F引物，SEQ ID NO：2的NLL-R引物，Cry3A-F引物 SEQ ID NO：6的Cry3A-R引物，SEQ ID NO：7的Cry3A-R引物和SEQ ID NO：8的Cry3A-F2引物。 SEQ ID NO：9的Cyr3A-pb探针与用于检测转基因植物的耐昆虫引物组一起使用。 SEQ ID NO：14的UGP-pb探针与用于检测转基因植物的耐昆虫引物组一起使用。 用于检测GMO植物如马铃薯的昆虫和病毒耐受性引物组包含一种或两种引物，其选自SEQ ID NO：3的NLYL-F引物，SEQ ID NO：4的NLYM-R引物和NLPS-R引物 SEQ ID NO：5。 用于检测天然或GMO植物的UDP-葡萄糖磷酸化酶基因的引物组包含选自SEQ ID NO：10的UGP-F引物，SEQ ID NO：11的UGP-R引物，SEQ ID NO：11的UGP-R引物，UGP-F2引物， SEQ ID NO：12和SEQ ID NO：13的UGP-R2引物。 标准质粒pCryugp用于定性和定量检测转基因植物。
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