公开(公告)号：KR1020040020107A

公开(公告)日：2004-03-09

申请号：KR1020020051559

申请日：2002-08-29

Applicant: 재단법인서울대학교산학협력재단

Inventor： 장준혁 ,  정종평 ,  구영

IPC: C07K14/50

CPC classification number: C07K14/71 ,  C07K16/2863 ,  G01N33/57426 ,  G01N33/57484

Abstract: PURPOSE: A protein FGFR2 isomer specifically expressed from cancer cells is provided, thereby effectively diagnosing cancer using an antibody specific to the protein FGFR2 isomer. CONSTITUTION: A protein FGFR2 isomer specifically is expressed from cancer cells and has the amino acid sequence set forth in SEQ ID NO: 4 in which exons 7 to 10 are deleted. The protein specifically binds to FGF1, FGF2 or FGF7 and induces the activation of AKT or MAPK. A gene encoding the protein FGFR2 isomer has the nucleotide sequence set forth in SEQ ID NO: 3. An antibody specifically binding to the protein FGFR2 isomer is provided. A kit for diagnosis of cancer comprises the antibody to the protein FGFR2 isomer.

Abstract translation: 目的：提供从癌细胞特异性表达的蛋白FGFR2异构体，从而使用对蛋白FGFR2异构体特异的抗体有效地诊断癌症。 构成：蛋白质FGFR2异构体特异性地从癌细胞表达，并且具有SEQ ID NO：4所示的氨基酸序列，其中缺失外显子7至10。 蛋白质特异性结合FGF1，FGF2或FGF7，并诱导AKT或MAPK的活化。 编码蛋白FGFR2异构体的基因具有SEQ ID NO：3所示的核苷酸序列。提供了与蛋白质FGFR2异构体特异性结合的抗体。 用于诊断癌症的试剂盒包含对蛋白质FGFR2异构体的抗体。

公开(公告)号：KR1020040014006A

公开(公告)日：2004-02-14

申请号：KR1020020047170

申请日：2002-08-09

Applicant: 재단법인서울대학교산학협력재단

Inventor： 장준혁 ,  정종평 ,  허성주 ,  김현이

IPC: C07K7/08

CPC classification number: C07K7/08 ,  C07K5/0806 ,  C07K5/10 ,  C07K7/06

Abstract: PURPOSE: Oligosaccharides specifically binding to hydroxyapatite are provided, which oligosaccharides are useful as linkers between biomolecules for increasing the affinity of hydroxyapatite to human body materials. CONSTITUTION: Oligosaccharides specifically binding to hydroxyapatite are provided, wherein the oligosaccharides have the amino acid sequence selected from the SEQ ID NO: 1 to SEQ ID NO: 17; the oligosaccharides are selected by screening peptides specifically binding to hydroxyapatite using peptide library; and the hydroxyapatite has similar chemical components and structure to the human bone.

Abstract translation: 目的：提供与羟基磷灰石特异性结合的寡糖，这些寡糖可用作生物分子之间的接头，用于增加羟基磷灰石与人体材料的亲和力。 提供：提供与羟基磷灰石特异性结合的寡糖，其中寡糖具有选自SEQ ID NO：1至SEQ ID NO：17的氨基酸序列; 通过使用肽文库筛选特异性结合羟基磷灰石的肽来选择寡糖; 羟基磷灰石具有与人骨相似的化学成分和结构。

公开(公告)号：KR1020030087278A

公开(公告)日：2003-11-14

申请号：KR1020020025274

申请日：2002-05-08

Applicant: 재단법인서울대학교산학협력재단

Inventor： 장준혁 ,  정종평 ,  이승진

IPC: C07K7/06

CPC classification number: C07K7/06 ,  C12N15/1086

Abstract: PURPOSE: An oligopeptide specifically binding to chitosan is provided. The oligopeptide can be useful for selective separation of biocompatible chitosan and useful as a linker between chitosan and biomolecules. CONSTITUTION: An oligopeptide specifically binding to chitosan is provided, wherein the oligopeptide has the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The oligopeptide specifically binding to chitosan is isolated by inserting random amino acid sequences into a vector pFLITRIX containing the active portion of thioredoxin; transforming Escherichia coli with the vector pFLITRIX; culturing the transformed E. coli in an ampicillin containing IMC selection medium; adding tryptophane into the medium and culturing it to induce FLITRIX peptide; binding the transformed E. coli to chitosan fixed on a dish; selecting peptides specifically binding to PLGA; and sequencing the selected peptides.

Abstract translation: 目的：提供特异性结合壳聚糖的寡肽。 寡肽可用于生物相容性壳聚糖的选择性分离，可用作壳聚糖与生物分子之间的接头。 提供了与壳聚糖特异性结合的寡肽，其中寡肽具有SEQ ID NO：1，SEQ ID NO：2或SEQ ID NO：3的氨基酸序列。通过插入随机氨基酸来分离与壳聚糖特异性结合的寡肽 酸序列转化成含有硫氧还蛋白活性部分的载体pFLITRIX; 用载体pFLITRIX转化大肠杆菌; 在含有氨苄青霉素的IMC选择培养基中培养转化的大肠杆菌; 将色氨酸加入到培养基中并培养以诱导FLITRIX肽; 将转化的大肠杆菌与固定在培养皿上的壳聚糖结合; 选择与PLGA特异性结合的肽; 并对所选择的肽进行测序。