公开(公告)号：EP2265709A2

公开(公告)日：2010-12-29

申请号：EP09763021.4

申请日：2009-03-27

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  PHARKYA, Priti ,  OSTERHOUT, Robin, E.

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6- aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：EP2427544B1

公开(公告)日：2019-07-17

申请号：EP10772921.2

申请日：2010-05-07

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10 ,  C12P13/00

公开(公告)号：EP2265709B1

公开(公告)日：2017-11-08

申请号：EP09763021.4

申请日：2009-03-27

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  PHARKYA, Priti ,  OSTERHOUT, Robin, E.

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

公开(公告)号：EP2773767A1

公开(公告)日：2014-09-10

申请号：EP12845401.4

申请日：2012-11-02

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti ,  BURK, Mark, J.

IPC: C12P17/02

CPC classification number: C12P17/08 ,  C07D313/04 ,  C08G18/4277 ,  C08G63/08 ,  C12N5/0018 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P17/02

Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：EP2475775A1

公开(公告)日：2012-07-18

申请号：EP10816105.0

申请日：2010-09-09

Applicant: Genomatica, Inc.

Inventor： PHARKYA, Priti ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  BURK, Mark, J. ,  SUN, Jun

IPC: C12P7/04 ,  C12N15/74

CPC classification number: C12P7/40 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18

Abstract: The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13- BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanolpathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.

公开(公告)号：EP2427544A1

公开(公告)日：2012-03-14

申请号：EP10772921.2

申请日：2010-05-07

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：EP2285973A2

公开(公告)日：2011-02-23

申请号：EP09739876.2

申请日：2009-04-30

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12P7/52 ,  C12N1/00 ,  C12P7/40

CPC classification number: C12P7/42 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/88 ,  C12N9/90 ,  C12P7/40 ,  C12Y102/01 ,  C12Y206/01022 ,  C12Y403/01 ,  C12Y504/99002

Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

公开(公告)号：EP2888369B1

公开(公告)日：2020-07-15

申请号：EP13832246.6

申请日：2013-08-27

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  VAN DIEN, Stephen, J. ,  TRACEWELL, Cara, Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC: C12P7/18 ,  C12N1/21 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/88

公开(公告)号：EP2462221B1

公开(公告)日：2017-02-22

申请号：EP10807185.3

申请日：2010-08-05

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

公开(公告)号：EP2756082A1

公开(公告)日：2014-07-23

申请号：EP12831648.6

申请日：2012-09-14

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E.

IPC: C12N15/74

CPC classification number: C12P5/026 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/1235 ,  C12N9/88 ,  C12N15/52 ,  C12P5/002 ,  C12Y402/03

Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.