公开(公告)号：EP3504291B1

公开(公告)日：2020-10-28

申请号：EP17765293.0

申请日：2017-08-24

Applicant: Life Technologies Corporation

Inventor： YORK, Adam ,  WELCH, Eric ,  GEE, Kyle ,  WANG, Xin ,  AGGELER, Robert ,  HU, Yi-Zhen

IPC: C09K11/02 ,  C09K11/06 ,  C08G61/10 ,  C08G61/12 ,  G01N33/533

公开(公告)号：EP3500860A1

公开(公告)日：2019-06-26

申请号：EP17758368.9

申请日：2017-08-16

Applicant: Life Technologies Corporation

Inventor： BEACHAM, Daniel ,  GEE, Kyle ,  RUKAVISHNIKOV, Aleksey

IPC: G01N33/68 ,  C07D493/10

公开(公告)号：EP2688867B1

公开(公告)日：2018-09-05

申请号：EP12714113.3

申请日：2012-03-22

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  CHEN, Aimei ,  KANG, Hee Chol

IPC: C07C309/42 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07D225/08 ,  C07K16/18 ,  G01N33/532

CPC classification number: C07D225/08 ,  C07C309/42 ,  C07C2603/36 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07K1/13 ,  C07K16/18 ,  G01N33/532

Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.

公开(公告)号：EP3344782A1

公开(公告)日：2018-07-11

申请号：EP16771026.8

申请日：2016-08-30

Applicant: Life Technologies Corporation

Inventor： CLARKE, Scott ,  GEE, Kyle ,  FINNESSY, Carmen

IPC: C12Q1/68

CPC classification number: A61K49/0043 ,  A61K49/0054 ,  C12Q1/6806 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/1025

Abstract: The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.

公开(公告)号：EP3314257A1

公开(公告)日：2018-05-02

申请号：EP16736957.8

申请日：2016-06-20

Applicant: Life Technologies Corporation

Inventor： MELQUIST, Penny ,  GEE, Kyle

IPC: G01N33/50 ,  G01N33/58 ,  C07D311/16 ,  C07D311/54 ,  C07F9/655 ,  C09B57/02

CPC classification number: G01N33/5005 ,  C09B57/02 ,  G01N33/582

Abstract: Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are coumarin-based cell-tracking reagents that fluoresce in the blue portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, good retention within cells and low toxicity toward cells.

公开(公告)号：EP2635350B1

公开(公告)日：2018-03-07

申请号：EP11838687.9

申请日：2011-11-01

Applicant: Life Technologies Corporation

Inventor： KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar ,  YING, Lai-Qiang ,  BRANCHAUD, Bruce

IPC: A61Q5/10 ,  A61K8/41 ,  C09B23/08

CPC classification number: G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

公开(公告)号：EP2831179A1

公开(公告)日：2015-02-04

申请号：EP13709645.9

申请日：2013-02-26

Applicant: Life Technologies Corporation

Inventor： HU, Yi-Zhen ,  CHEN, Aimei ,  KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar

IPC: C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/533 ,  G01N33/58

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either
in vitro and/or
in vivo . Also described herein are processes for preparing novel reduced dyes,
i.e ., ROS probes, for use in the disclosed compositions, methods and kits.

公开(公告)号：EP2807489A1

公开(公告)日：2014-12-03

申请号：EP13702569.8

申请日：2013-01-25

Applicant: Life Technologies Corporation

Inventor： ERGY, Levente ,  GEE, Kyle

IPC: G01N33/68

CPC classification number: G01N33/6803 ,  C07F5/022 ,  C09B23/04 ,  C09B57/00 ,  G01N33/52 ,  G01N33/582 ,  G01N33/68

Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.

Abstract translation: 根据本教导，提供了利用作为二吡咯亚甲基二氟化物化合物的染料的蛋白质熔融分析的系统，组合物，试剂盒和方法。 在一些实施方案中，一种方法包括通过将至少一种蛋白质与染料混合并在记录样品的荧光发射的同时施加受控加热来制备样品。

公开(公告)号：EP2681196A1

公开(公告)日：2014-01-08

申请号：EP12710600.3

申请日：2012-03-01

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott

IPC: C07D213/78

CPC classification number: G01N33/582 ,  C07D213/53 ,  C07D213/68 ,  C07D213/74 ,  C07D213/78 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D235/14 ,  C07D401/04 ,  C07D401/14 ,  C07K14/575 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  G01N33/533

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

公开(公告)号：EP3833715A2

公开(公告)日：2021-06-16

申请号：EP19762506.4

申请日：2019-08-08

Applicant: Life Technologies Corporation

Inventor： YANG, Jongtae ,  WANG, Xin ,  HU, Yi-Zhen ,  GEE, Kyle ,  CHEN, Aimei ,  BENSON, Scott ,  EVANS, Brian ,  RUKAVISHNIKOV, Aleksey

IPC: C09B69/00 ,  C09B11/14 ,  G01N33/58 ,  C12Q1/6869