公开(公告)号：JP2009287998A

公开(公告)日：2009-12-10

申请号：JP2008139193

申请日：2008-05-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： USHIO KOJI ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO

IPC: G01N27/327 ,  G01N27/28 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To provide a manufacturing method of a cell electrophysiological sensor with high measurement accuracy. SOLUTION: This method include a process to arrange a sensor chip 14 to the end of a glass tube 13, and a process of extrusion molding of the glass tube 13 while heating by a die 28 arranged outside, bending the glass tube 13 internally, and welding the glass tube to the sensor chip 14. Thereby, the periphery of the sensor chip 14 can be surrounded with the hydrophilic glass tube 13, and as a result, the cell electrophysiological sensor with high measurement accuracy can be manufactured. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供具有高测量精度的细胞电生理传感器的制造方法。 解决方案：该方法包括将传感器芯片14布置在玻璃管13的端部的过程，以及在由外部布置的模具28加热的同时玻璃管13的挤出成型的过程，弯曲玻璃管13 内部，并将玻璃管焊接到传感器芯片14.由此，传感器芯片14的周围可以被亲水性玻璃管13包围，结果可以制造具有高测量精度的细胞电生理传感器。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009198180A

公开(公告)日：2009-09-03

申请号：JP2008036821

申请日：2008-02-19

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： TAKAHASHI MAKOTO ,  NAKATANI MASAYA ,  USHIO KOJI

IPC: G01N27/28 ,  C12M1/34 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To enhance the measuring the accuracy of a cell electrophysiologic sensor. SOLUTION: The cell electrophysiologic sensor is equipped with a cell-holding plate 13, having a through-hole 22 and keep a groove 24 located in the outer periphery of the through-hole 22, in at least either the upper or under surface of the cell holding plate 13. Accordingly, with this constitution, dust can be dispersed even to the groove 24, and as a result, the measuring precision of the cell electrophysiologic sensor can be enhanced. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提高测量细胞电生理传感器的准确性。 解决方案：细胞电生理传感器配备有细胞保持板13，其具有通孔22，并且保持位于通孔22的外周中的凹槽24，至少在上部或下部 因此，通过这种结构，灰尘甚至可以分散到凹槽24，结果可以提高电池电生理传感器的测量精度。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009195107A

公开(公告)日：2009-09-03

申请号：JP2008036820

申请日：2008-02-19

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： USHIO KOJI ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO

IPC: C12M1/34 ,  G01N27/00 ,  G01N27/28 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To provide a cellular electrophysiological sensor capable of carrying out the measurement in a state of little leakage current even when the number of cells of measuring targets is increased. SOLUTION: The cellular electrophysiological sensor contacting a sensor chip 4 includes: a well 1 having a first through-hole 5; a holding plate 2 having a second through-hole 6 contacted downward of the well 1; and a diaphragm 9 having a third through-hole 7 in the inside of the second through-hole 6 by contacting a passage plate 3 having a cavity 11 having an inlet opening 16 and an outlet opening 17 of fluid downward of the holding plate 2 in both the ends. The holding plate 2 is made of a glass having hydrophilicity, and the sensor chip 4 is made of silicon. A stepped part 8 is formed at a part of the second through-hole 6 of the holding plate 2, and one surface of the stepped part 8 is joined to one surface of the sensor chip 4 by glass welding. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：即使当测量目标的细胞数量增加时，提供能够以几乎没有漏电流的状态进行测量的细胞电生理传感器。 传感器芯片4接触的细胞电生理传感器包括：具有第一通孔5的孔1; 具有与井1的下方接触的第二通孔6的保持板2; 以及隔膜9，其在第二通孔6的内部具有第三通孔7，通过使具有入口开口16的空腔11和保持板2的向下流体的出口17的通道板3接触 两端。 保持板2由具有亲水性的玻璃制成，传感器芯片4由硅制成。 台阶部分8形成在保持板2的第二通孔6的一部分，台阶部分8的一个表面通过玻璃焊接在传感芯片4的一个表面上。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009002876A

公开(公告)日：2009-01-08

申请号：JP2007165802

申请日：2007-06-25

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： TAKAHASHI MAKOTO ,  NAKATANI MASAYA ,  USHIO KOJI

IPC: G01N27/416 ,  G01N27/28 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To facilitate the suppression and removal of air bubbles, and to raise the reliability related to the measurement of a cell electrophysiology sensor. SOLUTION: The cell electrophysiology sensor includes a diaphragm 2 having at least one through hole 1, and a frame 3 that supports this diaphragm 2 and has a cavity 4. Since the shape of an end 5 of the frame 3 is made to have different heights, and the occurrence of air bubbles can be suppressed and the removal of the remaining air bubbles is facilitated. Therefore, the reliability in the measurement by the cell electrophysiology sensor can be raised. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了促进气泡的抑制和去除，并且提高与细胞电生理学传感器的测量相关的可靠性。 解决方案：细胞电生理学传感器包括具有至少一个通孔1的隔膜2和支撑该隔膜2并具有空腔4的框架3.由于框架3的端部5的形状被制成 具有不同的高度，并且可以抑制气泡的发生，并且便于除去剩余的气泡。 因此，可以提高细胞电生理学传感器的测量的可靠性。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008304304A

公开(公告)日：2008-12-18

申请号：JP2007151404

申请日：2007-06-07

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： NAKATANI MASAYA ,  USHIO KOJI ,  HIRAOKA SOICHIRO ,  OSHIMA AKIYOSHI ,  TAKAHASHI MAKOTO

IPC: G01N27/28 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To solve the problem that, as conventionally a micropipette is attached to an electrode holder with a screw and an O ring to ensure airtightness, such a method makes the work complicated and reduces the measurement efficiency. SOLUTION: The microprobe includes: a micropipette 10; a socket 7 having a first opening part 8 and a second opening part 9; a measuring electrode 4; a sleeve 2; and a connection member 3 provided with a pressure connection port 5, wherein, the back end of the micropipette 10 is inserted into the first opening part 8 of the socket 7, and the sleeve 2 is inserted into the second opening part 9 of this socket 7. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 解决的问题为了解决这样一个问题，像以往那样将微量移液器安装在具有螺钉和O形环的电极座上以确保气密性，这种方法使工作变得复杂并且降低了测量效率。 解决方案：微探针包括：微量移液管10; 具有第一开口部8和第二开口部9的插座7; 测量电极4; 套筒2; 以及设置有压力连接口5的连接部件3，其中，微量吸管10的后端插入到插座7的第一开口部分8中，套筒2插入该插座的第二开口部分9中 7.版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2012141142A

公开(公告)日：2012-07-26

申请号：JP2010291935

申请日：2010-12-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMADA YOSHIKI ,  NAKATANI MASAYA

IPC: G01N33/543 ,  C07K17/00 ,  C12M1/00 ,  C12N11/00

Abstract: PROBLEM TO BE SOLVED: To provide a probe immobilization method in which probe modification is restricted.SOLUTION: A probe immobilization method for fixing a probe 14 onto a substrate 11 comprises the steps of: preparing a substrate 11 to which DNA 12 is coupled and a probe 14 to which DNA-coupled protein 13 is coupled; and fixing the probe 14 via the DNA 12 at the substrate 11. The DNA 12 is thus formed on the substrate 11, and the DNA 12 is coupled to a DNA-coupling domain, which is part of the DNA-coupled protein 13 coupled to the probe 14, allowing the probe 14 to be adsorbed and fixed to the substrate, thereby making it possible to restrict the probe 14 from being modified with change in pH and temperature in a chemical reaction.

Abstract translation: 要解决的问题：提供限制探针修饰的探针固定方法。 解决方案：用于将探针14固定到基底11上的探针固定方法包括以下步骤：制备与DNA 12偶联的底物11和与DNA偶联蛋白13偶联的探针14; 并且通过DNA 12在底物11上固定探针14.DNA 12因此形成在底物11上，并且DNA 12与DNA偶联结构域偶联，DNA偶联结构域是DNA偶联蛋白13的一部分， 探针14，允许探针14被吸附并固定到基底上，从而可以限制探针14在化学反应中pH和温度的变化而被改变。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2012141140A

公开(公告)日：2012-07-26

申请号：JP2010291932

申请日：2010-12-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMADA YOSHIKI ,  TANEMURA AKINA ,  NAKATANI MASAYA

IPC: G01N33/543 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To provide a probe immobilization method in which probe modification is restricted.SOLUTION: A probe immobilization method for immobilizing a probe 12 onto a substrate 11 comprises the steps of: preparing the substrate 11 and the probe 12; and processing the substrate 11 to fix the probe 12 onto the substrate 11 without chemical reaction. This allows the probe 12 to be adsorbed and fixed to the substrate 11 in a natural reaction without any chemical reaction upon fixing the probe 12, thereby making it possible to restrict the probe 12 from being modified with change in pH and temperature in a chemical reaction.

Abstract translation: 要解决的问题：提供限制探针修饰的探针固定方法。 解决方案：用于将探针12固定到基底11上的探针固定方法包括以下步骤：制备基底11和探针12; 并且处理基板11以将探针12固定在基板11上而没有化学反应。 这样就可以使探针12在固定探针12时以自然的反应被吸附固定在基板11上而没有任何化学反应，从而可以限制探针12在化学反应中的pH和温度的变化中被改变 。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2011209174A

公开(公告)日：2011-10-20

申请号：JP2010078440

申请日：2010-03-30

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： NAKANO YUSUKE ,  NAKATANI MASAYA ,  YAMAMOTO KENJU

IPC: G01N27/28 ,  C12M1/34 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To improve production efficiency by improving a manufacturing yield of a cell electrophysiologic sensor.SOLUTION: The cell electrophysiologic sensor comprises: a sensor chip 13 that comprises a thin plate 16 including a through hole 18 and a frame body 17 held at the periphery of an upper surface of the thin plate 16 while surrounding the through hole 18 and having a cavity constituted of the frame body 17 and the through hole 18; and a well 11 abutting on the frame body 17 of the sensor chip 13 and holding the sensor chip 13, and keeping the outer peripheral part of the frame body 17 of the sensor chip 13 bonded to the inner peripheral part of the well 11 by an adhesive 20. The sensor has a constitution that a frame body gap 19a is opened at the outer peripheral part is formed in the frame body 17. Thus, the cell electrophysiologic sensor can improve a product yield by an outflow of the surplus adhesive 20 when bonded to a cavity.

Abstract translation: 要解决的问题：通过提高细胞电生理传感器的制造成品率来提高生产效率。解决方案：细胞电生理传感器包括：传感器芯片13，其包括薄板16，薄板16包括通孔18和保持在 薄板16的上表面的周边，同时围绕通孔18并具有由框体17和通孔18构成的空腔; 以及与传感器芯片13的框体17抵接并保持传感器芯片13，并且将传感器芯片13的框体17的外周部分保持在井11的内周部的外壁部分11 该传感器具有在框架体17中形成有在框架体17上开设框架体间隙19a的结构。因此，电池电生理传感器可以通过多余粘合剂20的流出而提高产品收率 到一个空腔。

公开(公告)号：JP2010213668A

公开(公告)日：2010-09-30

申请号：JP2009067413

申请日：2009-03-19

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMAMOTO KENJU ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO

IPC: C12M1/34 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To suppress the production cost of a chip, while maintaining the high measuring accuracy of a sensor.
SOLUTION: The biochip includes a diaphragm 22 having a first layer 24 and a second layer 25 installed on the first layer 24, and a substrate body 23 installed on the second layer 25 of the diaphragm 22, and also a recess (23A) so as to expose the second layer 24 at least at one of the substrate body 23 or the first layer 24, wherein the first layer 24 is made of a poly-crystalline silicon, an amorphous silicon or a mixture of the amorphous silicon with the poly-crystalline silicon, and the second layer 25 contains silicon dioxide as a main component, and the thickness of the first layer 24 is made smaller than that of the substrate body 23. Thus, it is possible to suppress the production cost of the chip 13, while maintaining the high accuracy of the sensor.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：在保持传感器的高测量精度的同时，抑制芯片的生产成本。 解决方案：生物芯片包括具有安装在第一层24上的第一层24和第二层25以及安装在隔膜22的第二层25上的衬底本体23以及凹部（23A）的隔膜22 ）以至少在衬底主体23或第一层24中的一个上露出第二层24，其中第一层24由多晶硅，非晶硅或非晶硅与 多晶硅，第二层25含有二氧化硅作为主要成分，第一层24的厚度比基板23的厚度小。因此，可以抑制芯片的制造成本 同时保持传感器的高精度。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2010183900A

公开(公告)日：2010-08-26

申请号：JP2009156945

申请日：2009-07-01

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： NAKATANI MASAYA ,  TAKAHASHI MAKOTO ,  USHIO KOJI ,  DANIEL YAZBECK

IPC: C12M1/00 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00509 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6825 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/507

Abstract: PROBLEM TO BE SOLVED: To provide a sensor enhanced in sensitivity which can be used besides DNA sensors.
SOLUTION: The sensor comprises a substrate having a surface formed of silicon, a plurality of fibrous protrusions formed of silicon dioxide and directly bonded to the surface formed of silicon of the surface and a plurality of functional molecule receptors or reactive groups each formed on the fibrous protrusions.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供除了DNA传感器之外可以使用的增强灵敏度的传感器。 解决方案：传感器包括具有由硅形成的表面的基底，多个由二氧化硅形成并直接结合到由表面的硅形成的表面的纤维突起，以及多个功能性分子受体或反应性基团 在纤维突起上。 版权所有（C）2010，JPO＆INPIT