公开(公告)号：WO1997047967A1

公开(公告)日：1997-12-18

申请号：PCT/US1997009801

申请日：1997-06-16

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  SOUTHGATE, Peter, David ,  LOEWY, Zvi, Gerald

IPC: G01N33/48

CPC classification number: C12N15/1013 ,  B01L3/50273 ,  B01L3/502738 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2300/021 ,  B01L2300/024 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/25375 ,  Y10T436/255

Abstract: The invention relates to a device and a method of use for that device for the automated preparation of nucleic acid from a biological sample. The device includes a cassette in which all chemical manipulations occur, which has a storage/transfer strip (380) where the prepared nucleic acid is deposited and stored without contamination. Moreover, the waste of the preparation protocol is contained in the cassette, which, upon separation of the filled storage/transfer strip (380) is completely sealed off from the environment. In a preferred embodiment, the inventive device can prepare nucleic acid preparations from up to 24 different biological samples at a time.

Abstract translation: 本发明涉及用于从生物样品自动制备核酸的该装置的装置和方法。 该装置包括其中发生所有化学操作的盒，其具有存储/转移条（380），其中所制备的核酸被沉积并存储而没有污染。 此外，制备方案的浪费被包含在盒中，其在填充的存储/转印带（380）分离时完全与环境密封。 在优选的实施方案中，本发明的装置可以一次从多达24个不同的生物样品制备核酸制剂。

公开(公告)号：WO1997047769A1

公开(公告)日：1997-12-18

申请号：PCT/US1997009802

申请日：1997-06-16

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  KUMAR, Rajan

IPC: C12Q01/68

CPC classification number: C12Q1/6834 ,  C12Q2561/125

Abstract: The invention relates to the detection of a polynucleotide using a padlock probe, whereby a hybridization process is combined with ligation to irreversibly capture polynucleotides of interest. The invention relates both to the padlock probe itself, as well as to methods of use, which allow for varying levels of sensitivity due to amplification of signal processes built into the system. Additionally, preferred formats are disclosed for use of the padlock probes, including the use of microfluidics.

Abstract translation: 本发明涉及使用挂锁探针检测多核苷酸，由此将杂交方法与连接组合以不可逆地捕获感兴趣的多核苷酸。 本发明涉及挂锁探针本身以及使用方法，其允许由于内置于系统中的信号处理的放大而导致变化的灵敏度水平。 此外，公开了使用挂锁探针的优选格式，包括使用微流体。

公开(公告)号：WO1997047761A1

公开(公告)日：1997-12-18

申请号：PCT/US1997009664

申请日：1997-06-13

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  KUMAR, Rajan ,  HEANEY, Paul

IPC: C12P19/34

CPC classification number: C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/155

Abstract: The present invention is a method of sequencing polynucleotides by sequential step sequencing. Sequential step sequencing begins with a single-stranded polynucleotide template that is annealed with a primer forming a primer-template complex. In one embodiment of the method, one labeled nucleotide at a time is added to the primer-template complex. Alternatively, this method can be used with a nucleotide or nucleotide analog that does not stop addition after a first nucleotide or nucleotide analog has been added to the primer. Other embodiments of the invention involve identification of polynucleotides having a contiguous non-redundant string or a superimposed non-redundant string pattern. The detection of a non-redundant contiguous string can be used to identify a particular gene. Alternatively, if the non-redundant contiguous string is not unique to a particular gene, the string can be used to form a DNA library that can then be searched with a second string. Similarly, a superimposed non-redundant string pattern can be used to identify a gene or to search a DNA library. Such strings can be used in annealing reactions and in computer searches of a database having a catalog of sequenced polynucleotides.

Abstract translation: 本发明是通过顺序步骤测序对多核苷酸进行测序的方法。 顺序步骤测序从与形成引物 - 模板复合物的引物退火的单链多核苷酸模板开始。 在该方法的一个实施方案中，一次将一个标记的核苷酸加入到引物 - 模板复合物中。 或者，该方法可以与在引物中加入第一个核苷酸或核苷酸类似物之后不停止添加的核苷酸或核苷酸类似物一起使用。 本发明的其它实施方案涉及鉴定具有连续非冗余串或叠加的非冗余字符串模式的多核苷酸。 非冗余连续字符串的检测可用于识别特定基因。 或者，如果非冗余连续字符串对于特定基因不是唯一的，则该字符串可用于形成DNA文库，然后可以用第二个字符串搜索。 类似地，可以使用叠加的非冗余字符串模式来鉴定基因或搜索DNA文库。 这样的串可用于具有测序多核苷酸目录的数据库的退火反应和计算机搜索。

公开(公告)号：WO1997039417A2

公开(公告)日：1997-10-23

申请号：PCT/US1997005224

申请日：1997-03-31

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  DeVRIES, Aalbert ,  LUBIN, Jeffrey ,  PETERSON, Heidi, A. ,  SPENCE, Clay

IPC: G06K00/00

CPC classification number: H04N19/198 ,  H04N19/124 ,  H04N19/126 ,  H04N19/142 ,  H04N19/146 ,  H04N19/147 ,  H04N19/154 ,  H04N19/17 ,  H04N19/172 ,  H04N19/179 ,  H04N19/192 ,  H04N19/196 ,  H04N19/42 ,  H04N19/60 ,  H04N19/61 ,  H04N19/87

Abstract: A signal processing apparatus (100, 400) and concomitant method for learning and using fidelity metric as a control mechanism and to process large quantities of fidelity metrics from a visual discrimination measure (VDM) (111) to a manageable subjective image quality ratings. The signal processing apparatus incorporates a VDM (111) and a neural network (112). The VDM (111) receives input image sequences and generates fidelity metrics, which are received by a neural network (112). The neural network (112) is trained to learn and use the fidelity metrics as a control mechanism, e.g., to control a video encoder.

Abstract translation: 一种用于学习和使用保真度量作为控制机制并且从视觉辨别度量（VDM）（111）到可管理的主观图像质量等级处理大量保真度量的信号处理设备（100,400）和伴随方法。 信号处理装置包括VDM（111）和神经网络（112）。 VDM（111）接收输入图像序列并产生由神经网络（112）接收的保真度量。 训练神经网络（112）以学习和使用保真度量作为控制机制，例如控制视频编码器。

公开(公告)号：WO1997038480A1

公开(公告)日：1997-10-16

申请号：PCT/US1997005352

申请日：1997-04-09

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  SUN, Hoi, Cheong, Steve ,  PLETCHER, Timothy, Allen

IPC: H02N13/00

CPC classification number: B05B5/087 ,  A61K9/2086 ,  A61K9/2893 ,  B01J2219/00468 ,  B05B5/08 ,  B05B5/082 ,  H02N13/00 ,  Y10T428/2991

Abstract: The present invention is directed to electrostatic chucks, methods for their use, the electrostatic deposition of objects, such as particles in a dry powder, onto recipient substrates, and the recipient substrates themselves that have been subjected to electrostatic deposition. In one aspect, the present invention provides an electrostatic chuck (620) for electrostatically attracting an object (680) or objects wherein the object is used in chemical or pharmaceutical assaying or manufacturing. The object can be pharmaceutical substrates, for example, such as a pharmaceutical tablet. Additional embodiments of the invention provide chucks and their use to electrostatically attract particles, such as pharmaceutical active ingredient, to a substrate, such as a tablet. In one aspect, the electrostatic chuck (1110) includes a floating electrode (1170), thereby providing for charge imaging for the deposition particles in a selected image. Additionally, the invention provides a sensing electrode (1310), optionally for use with an electrostatic chuck, for sensing the number of particles attracted to the objects on the chuck or other substrate, thereby providing for deposition of an accurate amount of particles. Furthermore, the present invention provides objects having selected areas in which particles are applied to the object via electrostatic means.

Abstract translation: 本发明涉及静电卡盘，它们的使用方法，诸如干粉末中的物体的静电沉积到受体基底上，以及已经经过静电沉积的受体底物本身。 一方面，本发明提供一种用于静电吸引物体（680）的静电卡盘（620）或其中物体用于化学或药物测定或制造中的物体。 该物体可以是药物底物，例如药物片剂。 本发明的另外的实施方案提供了卡盘及其用于静电吸引诸如药物活性成分的颗粒到基底如片剂的用途。 在一个方面，静电卡盘（1110）包括浮动电极（1170），从而为所选择的图像中的沉积颗粒提供电荷成像。 另外，本发明提供了可选地与静电卡盘一起使用的感测电极（1310），用于感测吸附到卡盘或其它基底上的物体的颗粒的数量，由此提供精确量的颗粒的沉积。 此外，本发明提供了具有通过静电装置将颗粒施加到物体的选择区域的物体。

公开(公告)号：WO1997037326A1

公开(公告)日：1997-10-09

申请号：PCT/US1997005225

申请日：1997-03-31

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  LUBIN, Jeffrey

IPC: G06T05/00

CPC classification number: H04N19/85 ,  H04N19/117 ,  H04N19/14 ,  H04N19/154 ,  H04N19/172 ,  H04N19/42 ,  H04N19/60

Abstract: An apparatus (400) and concomitant method for decomposing an image stream into units of local contrast. A temporal contrast transform (pyramid) comprises a spatial filter (410), a cascade of lowpass temporal filters (420) and a plurality of subtractors (430) and dividers (440). The spatial filter (410) is followed by a cascade of lowpass temporal filters (420), producing a set of temporally lowpass, spatially lowpass images. Adjacent filter outputs are then subtracted, producing a set of temporally bandpass (Laplacian) images. Finally, each Laplacian image is divided by a lower level temporal lowpass image, to produce a set of temporally bandpass contrast images.

Abstract translation: 一种用于将图像流分解成局部对比度单位的装置（400）和伴随方法。 时间对比度变换（金字塔）包括空间滤波器（410），级联的低通时间滤波器（420）和多个减法器（430）和分频器（440）。 空间滤波器（410）之后是级联的低通时间滤波器（420），产生一组时间上低通的空间上低通图像。 然后减去相邻的滤波器输出，产生一组时间带通（拉普拉斯算子）图像。 最后，每个拉普拉斯图像被下一级时间低通图像分割，以产生一组时间带通对比度图像。

公开(公告)号：WO1997037324A1

公开(公告)日：1997-10-09

申请号：PCT/US1997004663

申请日：1997-03-31

Applicant: SARNOFF CORPORATION

Inventor： SARNOFF CORPORATION ,  BRILL, Michael, Henry ,  VAN DER WAL, Gooitzen, Siemen ,  LUBIN, Jeffrey

IPC: G06T05/00

CPC classification number: G06T5/50 ,  H04N19/117 ,  H04N19/14 ,  H04N19/154 ,  H04N19/172 ,  H04N19/179 ,  H04N19/60 ,  H04N19/80 ,  H04N19/85

Abstract: An apparatus for assessing the visibility of differences between two input image sequences. The apparatus comprises a pair of pre-filtered processors (710), a pair of pyramid constructors (720) and a JND processor (740). Each pre-filter processor (710) receives a separate input image sequence (202, 204) and performs temporal filtering to produce a low-pass temporal response (A LOW and B LOW) and a bandpass temporal response (A HIGH and B HIGH). In turn, the pyramid constructors (720) receive the temporal responses and generate contrast pyramids (722, 724) for the temporal responses. The JND processor (740) evaluates the contrast pyramids (722, 724) to produce an image metric which is used to assess the visibility of differences between the two input image sequences.

Abstract translation: 用于评估两个输入图像序列之间的差异的可见性的装置。 该装置包括一对预过滤处理器（710），一对金字塔构造器（720）和JND处理器（740）。 每个预滤波器处理器（710）接收单独的输入图像序列（202,204）并且执行时间滤波以产生低通时间响应（A LOW和B LOW）和带通时间响应（A HIGH和B HIGH） 。 反过来，金字塔构造器（720）接收时间响应并产生用于时间响应的对比金字塔（722,724）。 JND处理器（740）评估对比金字塔（722,724）以产生用于评估两个输入图像序列之间的差异的可视性的图像度量。