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公开(公告)号：WO2009021233A3
公开(公告)日：2009-02-12
申请号：PCT/US2008/072770
申请日：2008-08-11
Applicant: ADVANCED LIQUID LOGIC, INC. , SRINIVASAN, Vijay , PAMULA, Vamsee , POLLACK, Michael
Inventor： SRINIVASAN, Vijay , PAMULA, Vamsee , POLLACK, Michael
IPC: H05K1/00
Abstract: Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
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公开(公告)号：WO2008106678A1
公开(公告)日：2008-09-04
申请号：PCT/US2008/055648
申请日：2008-03-03
Applicant: ADVANCED LIQUID LOGIC, INC. , PAMULA, Vamsee, K. , POLLACK, Michael, G. , SRINIVASAN, Vijay , PAIK, Phil
Inventor： PAMULA, Vamsee, K. , POLLACK, Michael, G. , SRINIVASAN, Vijay , PAIK, Phil
IPC: B41J2/045
CPC classification number: B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
Abstract: Approaches to configuring and wiring electrodes in a droplet actuator are provided. Droplet actuators employing the designs of the invention are useful for conducting a variety of droplet operations. In one set of embodiments, the droplet actuator of the invention includes various single-layer wiring configurations for mitigating the constraints and drawbacks that are associated with single-layer designs, such as wireability constraints, limited mechanisms for performing droplet operations, electrostatic interference from wires, and any combinations thereof. In another set of embodiments, the droplet actuator of the invention includes a reference electrode that is situated on one substrate that is separated by a gap from a second substrate and one or more control electrodes that are situated on the second substrate. The control electrodes may be placed such that the second substrate is interposed between the control electrodes and the first substrate.
Abstract translation: 提供了在液滴致动器中配置和布线电极的方法。 采用本发明设计的液滴致动器可用于进行各种液滴操作。 在一组实施例中，本发明的液滴致动器包括用于减轻与单层设计相关联的约束和缺点的各种单层布线配置，例如线性约束，用于执行液滴操作的有限机构，来自线的静电干扰 ，及其任何组合。 在另一组实施例中，本发明的液滴致动器包括参考电极，该参考电极位于一个衬底上，其间隔开第二衬底的间隙和位于第二衬底上的一个或多个控制电极。 控制电极可以被放置成使得第二衬底插入在控制电极和第一衬底之间。
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公开(公告)号：WO2007120240A3
公开(公告)日：2008-08-28
申请号：PCT/US2006047481
申请日：2006-12-11
Applicant: ADVANCED LIQUID LOGIC INC , UNIV DUKE , UNIV LELAND STANFORD JUNIOR , POLLACK MICHAEL G , PAMULA VAMSEE K , ALLEN DWAYNE J , FAIR RICHARD B , GRIFFIN PETER
Inventor： POLLACK MICHAEL G , PAMULA VAMSEE K , ALLEN DWAYNE J , FAIR RICHARD B , GRIFFIN PETER
CPC classification number: B01L3/502792 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/6869 , G01N33/49 , C12Q2565/629 , C12Q2565/301
Abstract: The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.
Abstract translation: 液滴微致动器技术领域本发明涉及液滴微致动器以及使用液滴微致动器来使用液滴执行各种协议的系统，装置和方法。 本发明包括液滴微致动器或液滴微致动器系统，其具有一个或多个输入储存器，其中装载有用于进行测序方案的试剂，例如用于进行焦磷酸测序方案的试剂。 本发明还包括液滴微致动器或液滴微致动器系统，其具有装载有用于进行焦磷酸测序方案的样品的一个或多个输入储存器。
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公开(公告)号：WO2008101194A2
公开(公告)日：2008-08-21
申请号：PCT/US2008/054134
申请日：2008-02-15
Applicant: ADVANCED LIQUID LOGIC, INC. , STURMER, Ryan A. , POLLACK, Michael G. , PAMULA, Vamsee K. , SRINIVASAN, Vijay , PAIK, Philip Y.
Inventor： STURMER, Ryan A. , POLLACK, Michael G. , PAMULA, Vamsee K. , SRINIVASAN, Vijay , PAIK, Philip Y.
IPC: G01R5/28
CPC classification number: B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤来确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。
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公开(公告)号：WO2007120241A2
公开(公告)日：2007-10-25
申请号：PCT/US2006/047486
申请日：2006-12-11
Applicant: ADVANCED LIQUID LOGIC, INC. , DUKE UNIVERSITY , POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay , PAIK, Philip, Y. , ECKHARDT, Allen, E. , FAIR, Richard, B.
Inventor： POLLACK, Michael, G. , PAMULA, Vamsee, K. , SRINIVASAN, Vijay , PAIK, Philip, Y. , ECKHARDT, Allen, E. , FAIR, Richard, B.
IPC: B01D57/00
CPC classification number: B01L3/502792 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/6869 , G01N33/49 , C12Q2565/629 , C12Q2565/301
Abstract: The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using discrete droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting biochemical reactions, such as the reagents described for use in nucleic acid amplification protocols, affinity-based assay protocols, sequencing protocols, and protocols for analyses of biological fluids.
Abstract translation: 液滴微致动器技术领域本发明涉及液滴微致动器以及使用液滴微致动器用于使用离散液滴执行各种协议的系统，设备和方法。 本发明包括具有装载有用于进行生物化学反应的试剂的一个或多个输入储存器的液滴微致动器或液滴微致动器系统，例如用于核酸扩增方案的所述试剂，基于亲和性的测定方案，测序方案和用于分析的方案 的生物液体。
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Patent Agency Ranking
公开(公告)号：WO2015010127A1
公开(公告)日：2015-01-22
申请号：PCT/US2014047449
申请日：2014-07-21
Applicant: ADVANCED LIQUID LOGIC INC
Inventor： SMITH GREGORY , LUCK RANDALL L
CPC classification number: G01K7/16 , B01L3/502792 , B01L2200/147 , B01L2300/0645 , B01L2300/0887 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0427 , B01L2400/043 , B01L2400/0433 , B01L2400/0442 , B01L2400/0457 , B01L2400/0478 , G01K13/00
Abstract: The present invention provides methods for on-actuator temperature measurement and temperature control, including where one or more of the temperature sensors are combined with one or more heaters that are formed of wiring traces and/or providing heaters designed for one-to- one correspondence to the temperature sensors to form temperature sensor-heater pairs. The present invention also provides methods for on-actuator temperature measurement and temperature control in which the temperature sensors comprise a connection comprising a plurality of terminals by which an amount of current can be applied and then a voltage measured, wherein the voltage that is measured across the temperature sensors can be accurately correlated to a temperature.
Abstract translation: 本发明提供了用于致动器温度测量和温度控制的方法，包括其中一个或多个温度传感器与由布线迹线形成的一个或多个加热器组合和/或提供设计用于一对一对应的加热器 到温度传感器形成温度传感器加热器对。 本发明还提供了用于致动器温度测量和温度控制的方法，其中温度传感器包括包括多个端子的连接，通过该连接可以施加电流量，然后测量电压，其中测量的电压 温度传感器可以准确地与温度相关。
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公开(公告)号：WO2014186697A2
公开(公告)日：2014-11-20
申请号：PCT/US2014/038374
申请日：2014-05-16
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： PUNNAMARAJU, Srikoundinya , SANDAHL, Melissa, A. , HARRINGTON, John, J. , ECKHARDT, Allen, E. , HUA, Zhishan , POLLACK, Michael, G.
IPC: C12M1/42
Abstract: The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.
Abstract translation: 本发明提供了一种液滴致动器，其设计用于在液滴中对细胞进行电穿孔。 本发明还提供了用于在液滴致动器上以液滴的细胞进行电穿孔的方法和系统。
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公开(公告)号：WO2014179596A1
公开(公告)日：2014-11-06
申请号：PCT/US2014/036407
申请日：2014-05-01
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： ECKHARDT, Allen, E. , POLLACK, Michael, G. , COHEN, David, S.
IPC: C12M1/34
CPC classification number: C12Q1/6869 , B01L7/52 , C12Q1/6844 , C40B20/00 , C12Q2533/101 , C12Q2565/301
Abstract: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.
Abstract translation: 本发明提供了基于焦磷酸测序的DNA分析和合成方法，包括DNA错误校正方法，确定DNA大小分布，筛选核苷酸重复序列如脆性X综合征，确定DNA文库中的大小分布和偏差，以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。
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公开(公告)号：WO2014106167A1
公开(公告)日：2014-07-03
申请号：PCT/US2013/078263
申请日：2013-12-30
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： POLLACK, Michael, G.
CPC classification number: C12Q1/686 , C12Q2563/159 , C12Q2565/629
Abstract: The invention provides methods for gene synthesis and error correction on a droplet actuator. In one embodiment, the invention provides a method for performing polymerase-mediated gene synthesis on a droplet actuator. The polymerase-mediated gene synthesis method uses oligonucleotide hybridization and PCR cycling to generate a pool of synthesized DNA strands, which may be subsequently enriched for the correct gene sequence.
Abstract translation: 本发明提供了用于液滴致动器的基因合成和纠错的方法。 在一个实施方案中，本发明提供了在液滴致动器上进行聚合酶介导的基因合成的方法。 聚合酶介导的基因合成方法使用寡核苷酸杂交和PCR循环来产生合成的DNA链的集合，其可以随后富集正确的基因序列。
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70.发明申请MECHANISMS FOR AND METHODS OF LOADING A DROPLET ACTUATOR WITH FILLER FLUID 审中-公开用于填充带有填充流体的液滴致动器的机构和方法
公开(公告)号：WO2014078100A1
公开(公告)日：2014-05-22
申请号：PCT/US2013/068080
申请日：2013-11-01
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： BORT, Donovan, E. , SISTA, Ramakrishna , LIN, Yan-You , SANSEVIERI, Christopher, D. , DE VILLE, Jarred
IPC: B01L3/00 , G01N33/543
CPC classification number: B01L3/502792 , B01L3/0275 , B01L2200/027 , B01L2200/0642 , B01L2200/0684 , B01L2400/0427
Abstract: The present invention is directed to mechanisms for and methods of loading a droplet actuator with filler fluid. The mechanisms and methods are useful for loading filler fluid in a droplet actuator in a manner that is rapid enough for practical use while at the same time sufficiently slow to reduce, preferably entirely avoid, the trapping of air in the droplet operations gap during the loading process.
Abstract translation: 本发明涉及用填充液加载液滴致动器的机构和方法。 这些机理和方法可用于将液体致动器中的填充流体以足够实际使用的速度加载，同时充分缓慢地减少，优选地完全避免在加载期间在液滴操作间隙内捕获空气的方式 处理。
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