公开(公告)号：WO2003040180A2

公开(公告)日：2003-05-15

申请号：PCT/EP2002/012138

申请日：2002-10-31

Applicant: BASF AKTIENGESELLSCHAFT ,  ZELDER, Oskar ,  POMPEJUS, Markus ,  SCHRÖDER, Hartwig ,  KRÖGER, BURKHARD ,  KLOPPROGGE, Corinna ,  HABERHAUER, Gregor

Inventor： ZELDER, Oskar ,  POMPEJUS, Markus ,  SCHRÖDER, Hartwig ,  KRÖGER, BURKHARD ,  KLOPPROGGE, Corinna ,  HABERHAUER, Gregor

IPC: C07K14/34

CPC classification number: C12N9/00 ,  C07K14/34 ,  C12N9/0006 ,  C12P13/08

Abstract: Die Erfindung betrifft neue Nukleinsäuremoleküle, deren Verwendung zur Konstruktion von gentechnisch verbesserten Mikroorganismen und Verfahren zur Herstellung von Feinchemikalien, insbesondere Aminosäuren mit Hilfe dieser gentechnisch verbesserten Mikroorganismen.

Abstract translation: 本发明涉及新的核酸分子，它们的遗传改良的微生物以及用于产生精细化学品，在使用这些遗传改良的微生物的特定氨基酸的方法的结构中使用。

公开(公告)号：WO2009133063A2

公开(公告)日：2009-11-05

申请号：PCT/EP2009/055051

申请日：2009-04-27

Applicant: EVONIK DEGUSSA GMBH ,  ZELDER, Oskar ,  SCHRÖDER, Hartwig ,  KLOPPROGGE, Corinna ,  HEROLD, Andrea ,  HAEFNER, Stefan

Inventor： ZELDER, Oskar ,  SCHRÖDER, Hartwig ,  KLOPPROGGE, Corinna ,  HEROLD, Andrea ,  HAEFNER, Stefan

IPC: C12P13/12

CPC classification number: C12P13/12

Abstract: The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of methionine.

Abstract translation: 本发明涉及利用具有降低的异柠檬酸脱氢酶活性的微生物来生产甲硫氨酸的方法。

公开(公告)号：WO2008080900A2

公开(公告)日：2008-07-10

申请号：PCT/EP2007/064471

申请日：2007-12-21

Applicant: BASF SE ,  ZELDER, Oskar ,  GRABARSE, Wolfgang ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  HAEFNER, Stefan ,  KNIETSCH, Anja ,  HEROLD, Andrea

Inventor： ZELDER, Oskar ,  GRABARSE, Wolfgang ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  HAEFNER, Stefan ,  KNIETSCH, Anja ,  HEROLD, Andrea

IPC: C07K14/43

CPC classification number: C12N9/0091 ,  C12P13/12

Abstract: The present invention relates to nulceotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof being modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of suchnucleotide sequences.

Abstract translation: 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核糖核酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品抑制蛋氨酸的产物减少。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。

公开(公告)号：WO2008049782A1

公开(公告)日：2008-05-02

申请号：PCT/EP2007/061152

申请日：2007-10-18

Applicant: BASF SE ,  HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  ZELDER, Oskar ,  JEONG, Weol Kyu

Inventor： HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  ZELDER, Oskar ,  JEONG, Weol Kyu

IPC: C12N15/67 ,  C12N15/77

CPC classification number: C12N15/67 ,  C12N15/77

Abstract: The present invention relates to a method of increasing the amount of at least one polypeptide in the host cell by expressing a modified nucleotide sequence encoding for a polypeptide in a host cell with said modified.nucleotide sequence being derived from a different non-modified nucleotide sequence encoding for a polypeptide of identical amino acid sequence such that the codon usage of the modified nucleotide sequence is adjusted to the codon usage of abundant proteins in the host cell.

Abstract translation: 本发明涉及一种通过在宿主细胞中表达编码多肽的修饰的核苷酸序列来增加宿主细胞中至少一种多肽的量的方法，所述修饰的核苷酸序列衍生自不同的未修饰的核苷酸序列 编码相同氨基酸序列的多肽，使得修饰的核苷酸序列的密码子使用被调整为宿主细胞中丰富蛋白质的密码子使用。

公开(公告)号：WO2008049781A1

公开(公告)日：2008-05-02

申请号：PCT/EP2007/061151

申请日：2007-10-18

Applicant: BASF SE ,  HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  ZELDER, Oskar ,  JEONG, Weol Kyu

Inventor： HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  ZELDER, Oskar ,  JEONG, Weol Kyu

IPC: C12N15/77

CPC classification number: C12N15/77

Abstract: The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.

Abstract translation: 本发明涉及通过在宿主细胞中表达编码多肽的核苷酸序列来减少宿主细胞中至少一种多肽的量的方法，其中所述核苷酸序列使用根据密码子使用的密码子使用的密码子 宿主生物体。 此外，本发明涉及编码具有密码子用途的多肽的核苷酸序列，其已被调整为使用根据宿主生物的密码子使用很少使用的密码子。 本发明还涉及这样的序列和方法用于生产精细化学品如氨基酸，糖，脂质，油，碳水化合物，维生素，辅因子等的用途。

公开(公告)号：WO2007051725A3

公开(公告)日：2007-06-28

申请号：PCT/EP2006067680

申请日：2006-10-23

Applicant: BASF AG ,  ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH ,  KROEMER JENS ,  PERO JANICE G ,  YOCUM R ROGERS ,  PATTERSON THOMAS A ,  WILLIAMS MARK ,  HERMAN THERON

Inventor： ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH ,  KROEMER JENS ,  PERO JANICE G ,  YOCUM R ROGERS ,  PATTERSON THOMAS A ,  WILLIAMS MARK ,  HERMAN THERON

IPC: A23K1/00 ,  C12N15/52 ,  C12P13/12

CPC classification number: C12P13/12 ,  A23K10/16 ,  A23K20/142 ,  A23K40/10 ,  C12N15/52

Abstract: The present invention relates to microorganisms and processes for the efficient preparation of L-amino acids such as L-methionine. In particular, the present invention relates to microorganisms and processes in which the formation and/or accumulation of homolanthionine in the methionine pathway is reduced and/or prevented.

Abstract translation: 本发明涉及用于有效制备L-氨基酸如L-甲硫氨酸的微生物和方法。 特别地，本发明涉及微生物和其中甲硫氨酸途径中高山ion素的形成和/或积累减少和/或预防的方法。

公开(公告)号：WO2007012078A1

公开(公告)日：2007-01-25

申请号：PCT/US2006/028439

申请日：2006-07-18

Applicant: BASF AG ,  ZELDER, Oskar ,  HAEFNER, Stefan ,  KLOPPROGGE, Corinna ,  SCHRODER, Hartwig ,  HEROLD, Andrea ,  PATTERSON, Thomas, A. ,  HERMANN, Theron ,  YOCUM, Rogers, R. ,  WILLIAMS, Mark, K. ,  PERO, Janice, G.

Inventor： ZELDER, Oskar ,  HAEFNER, Stefan ,  KLOPPROGGE, Corinna ,  SCHRODER, Hartwig ,  HEROLD, Andrea ,  PATTERSON, Thomas, A. ,  HERMANN, Theron ,  YOCUM, Rogers, R. ,  WILLIAMS, Mark, K. ,  PERO, Janice, G.

IPC: C12P13/12

CPC classification number: C12P13/12 ,  C12N15/52

Abstract: This invention relates to methionine producing recombinant microorganisms. Specifically, this invention relates to recombinant strains of Corynebacterium that produce increased levels of methionine compared to their wild-type counterparts and further to methods of generating such microorganisms.

Abstract translation: 本发明涉及产生甲硫氨酸的重组微生物。 具体而言，本发明涉及棒状杆菌的重组菌株，其与野生型对应物相比产生较高水平的甲硫氨酸，并且还涉及产生这种微生物的方法。

公开(公告)号：WO2007011845A2

公开(公告)日：2007-01-25

申请号：PCT/US2006/027617

申请日：2006-07-18

Applicant: BASF AG ,  ZELDER, Oskar ,  HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  YOCUM, R., Rogers ,  WILLIAMS, Mark, K.

Inventor： ZELDER, Oskar ,  HEROLD, Andrea ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  YOCUM, R., Rogers ,  WILLIAMS, Mark, K.

IPC: C12P13/12 ,  C07K14/32 ,  C12N5/10 ,  C07K14/34

CPC classification number: C12P13/12 ,  C07K14/32 ,  C07K14/34 ,  C12P3/00 ,  C12P5/023 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/28 ,  C12P7/48 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  C12P13/04 ,  C12P23/00 ,  Y02E50/10 ,  Y02E50/17 ,  Y02E50/343

Abstract: The present invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing fine chemicals using the metl gene from Bacillus subtilis or a gene related to metI . In some embodiments of the present invention, the metI gene or another gene is integrated in a fashion that allows for co-production of a water soluble compound such as methionine or other amino acid and a caortenoid compound.

Abstract translation: 本发明涉及使用来自枯草芽孢杆菌的met1基因或与metI相关的基因来生产甲硫氨酸和其它含硫精细化学品的改进的微生物和方法。 在本发明的一些实施方案中，metI基因或另一基因以允许水溶性化合物例如甲硫氨酸或其它氨基酸和类酮化合物共同产生的方式整合。

公开(公告)号：WO2006008097A2

公开(公告)日：2006-01-26

申请号：PCT/EP2005007752

申请日：2005-07-16

Applicant: BASF AG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N15/00

CPC classification number: C12N15/63 ,  C07K14/34 ,  C12N15/77 ,  C12P13/04

Abstract: The invention relates to the use of nucleic acid sequences for regulating gene transcription and expression, said novel promoters and expression units, methods for modifying or inducing the transcription rate and/or expression rate of genes, expression cassettes containing said expression units, genetically modified microorganisms with a modified or induced transcription rate and/or expression rate, and methods for producing biosynthetic products by cultivating the genetically modified microorganisms.

Abstract translation: 本发明涉及使用核酸序列的调节基因，所述新的启动子和表达单元本身的转录和表达，用于改变或引起转录和/或基因的表达率的速率的方法，含有该表达单元的表达盒，具有改变的遗传修饰的微生物或 引起转录速率和/或表达的速率以及通过培养遗传修饰的微生物的制备生物合成产物的方法。

公开(公告)号：WO2005121349A2

公开(公告)日：2005-12-22

申请号：PCT/IB2004/004463

申请日：2004-12-17

Applicant: BASF AKTIENGESELLSCHAFT ,  ZELDER, Oskar ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  HÄFNER, Stefan ,  KRÖGER, Burkhard ,  KIEFER, Patrick ,  HEINZLE, Elmar ,  WITTMANN, Christoph

Inventor： ZELDER, Oskar ,  KLOPPROGGE, Corinna ,  SCHRÖDER, Hartwig ,  HÄFNER, Stefan ,  KRÖGER, Burkhard ,  KIEFER, Patrick ,  HEINZLE, Elmar ,  WITTMANN, Christoph

IPC: C12P

CPC classification number: C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i, e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

Abstract translation: 本发明的特征在于通过使酶编码基因（即，甘油激酶）失调来增加精细化学品例如来自微生物例如棒状杆菌的赖氨酸的生产方法。 在优选的实施方案中，本发明提供了通过增加甘油激酶活性的表达来增加谷氨酸棒杆菌中赖氨酸的产生的方法。 本发明还提供了一种通过调节向草酰乙酸（OAA）的碳通量来生产赖氨酸的新方法。 在优选的实施方案中，本发明提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。