Fluorimetric detection system of nucleic acid
    70.
    发明专利
    Fluorimetric detection system of nucleic acid 审中-公开
    核酸荧光检测系统

    公开(公告)号:JP2009195238A

    公开(公告)日:2009-09-03

    申请号:JP2009103044

    申请日:2009-04-21

    Abstract: PROBLEM TO BE SOLVED: To provide a chain specific system for detecting the presence of a specific target nucleic acid sequence useful for PCR reaction, determination of an amount of a target sequence present in a sample, to generate duplex destabilization data such as a melt hysteresis information for amplification monitoring or for detection and quantitation of polymorphisms or allelic variation, and useful for general diagnosis. SOLUTION: Provided is the method for detecting the presence of the target nucleic acid sequence in the sample, the method comprising, (a) adding to the sample suspected of containing the target nucleic acid sequence, a probe specific for the target sequence and DNA duplex binding agent, the probe comprising a reactive molecule able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent, (b) subjecting thus formed mixture to an amplification reaction in which target nucleic acid is amplified, (c) subjecting the sample to conditions under which the probe hybridizes to the target sequence, and (d) monitoring fluorescence from the sample. COPYRIGHT: (C)2009,JPO&INPIT

    Abstract translation: 要解决的问题:提供用于检测有用于PCR反应的特异性目标核酸序列的存在的链特异性系统,确定样品中存在的靶序列的量,以产生双相不稳定数据,例如 用于扩增监测或用于多态性或等位基因变异的检测和定量的熔融滞后信息,并且可用于一般诊断。 提供了用于检测样品中靶核酸序列的存在的方法,所述方法包括:(a)向疑似含有靶核酸序列的样品中加入靶序列特异性的探针 和DNA双链体结合剂,所述探针包含能够吸收荧光能量或向DNA双链体结合剂提供荧光能的反应性分子,(b)使由此形成的混合物经受其中扩增靶核酸的扩增反应,(c) 对样品进行与靶序列杂交的条件,(d)监测来自样品的荧光。 版权所有(C)2009,JPO&INPIT

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