公开(公告)号：KR1020130067999A

公开(公告)日：2013-06-25

申请号：KR1020110135105

申请日：2011-12-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  이현정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈 ,  변미정

IPC: C12N9/24 ,  C12N15/56 ,  C12N15/63 ,  A23K1/16

Abstract: PURPOSE: A product of Cel9-KG10 gene, endoglucanase, is provided to be used as a feed additive and a detergent composition and to efficiently develop biofuel by finding a cellulase gene from the microorganism. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. The gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing endoglucanase.

Abstract translation: 目的：提供Cel9-KG10基因，内切葡聚糖酶的产物，用作饲料添加剂和洗涤剂组合物，并通过从微生物中发现纤维素酶基因有效地开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。该基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切葡聚糖酶的方法包括将重组载体转导至宿主细胞并过量表达内切葡聚糖酶的步骤。

公开(公告)号：KR1020130067997A

公开(公告)日：2013-06-25

申请号：KR1020110135103

申请日：2011-12-15

Applicant: 대한민국(농촌진흥청장)

Inventor： 이경태 ,  김태헌 ,  이현정 ,  안정현 ,  김영주 ,  안현승 ,  김지훈 ,  변미정

IPC: C12N9/24 ,  C12N15/56 ,  C12N15/63 ,  C12P21/00

CPC classification number: C12N9/2482 ,  A23K10/14 ,  C11D3/38636 ,  C12N15/67 ,  C12P7/649 ,  C12Y302/01008

Abstract: PURPOSE: Endo-1,4-beta-xylanase which is a product of cel10-KG04 gene is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: Endo-1,4-beta-xylanase derived from ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endo-1,4-beta-xylanase. A recombinant vector contains the gene. A host cell is transformed with the recombinant vector. A method for producing a large amount of endo-1,4-beta-xylanase from the host cell comprises a step of transducing a recombinant vector to the host cell and overexpressing endo-1,4-beta-xylanase.

Abstract translation: 目的：提供作为cel10-KG04基因产物的Endo-1,4-β-木聚糖酶用于饲料添加剂和洗涤剂组合物，并开发生物燃料。 构成：来源于黑山羊的反刍动物胃微生物的内切-1,4--β-木聚糖酶具有序列号2的氨基酸序列。一个基因编码内切-1,4-β-木聚糖酶。 重组载体含有该基因。 用重组载体转化宿主细胞。 用于从宿主细胞产生大量内切-1,4-β-木聚糖酶的方法包括将重组载体转导到宿主细胞并过量表达内切-β-β-木聚糖酶的步骤。