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    Process for metering, in particular microvolumes of a liquid; application to obtaining controlled dilutions, especially nanomolar dilutions
    63.
    发明授权
    Process for metering, in particular microvolumes of a liquid; application to obtaining controlled dilutions, especially nanomolar dilutions 失效
    用于计量的方法，特别是液体的微量体积; 用于获得受控稀释度，特别是纳摩尔稀释度

    公开(公告)号：US5594183A

    公开(公告)日：1997-01-14

    申请号：US272398

    申请日：1994-07-26

    Applicant: Bruno Colin

    Inventor： Bruno Colin

    IPC: B01L99/00 ,  C12M1/26 ,  G01F11/00 ,  G01N1/38 ,  G01N1/14

    CPC classification number: B01L3/0293 ,  C12M33/07 ,  G01F11/00 ,  G01N1/38

    Abstract: A capillary cavity is provided in communication with a liquid bath container. The cavity is metered with a predetermined quantity of a liquid by a process that includes: (a) providing at least one capillary cavity, which outwardly emerges via an introduction orifice and is dimensioned such that the liquid cannot enter or leave the cavity spontaneously; (b) providing a bath of the liquid; (c) evacuating the cavity, initially free of any liquid, in order to bring its internal pressure to a value that is less than a pressure prevailing above the liquid bath; (d) connecting the interior of the capillary cavity, which is at least partially evacuated, to the liquid bath via the introduction orifice, thereby filling the cavity; (e) separating the filled capillary cavity from a remainder of the liquid bath; (f) emptying the capillary cavity which is filled and separated from the liquid bath via the introduction orifice. The method and device have applications including, for example, diluting a precise amount of liquid with a diluent.

    Abstract translation: 提供与液浴容器连通的毛细管腔。 通过包括以下步骤的方法对空腔计量预定量的液体：（a）提供至少一个毛细管腔，其经由引入孔向外出现，并且尺寸使得液体不能自发地进入或离开空腔; （b）提供液体浴; （c）抽空空腔，最初没有任何液体，以使其内部压力达到小于液槽上方压力的值; （d）通过引入孔将至少部分抽真空的毛细管腔的内部连接到液槽，从而填充空腔; （e）将填充的毛细管腔与液体浴的其余部分分离; （f）通过引入孔排空从液槽中填充和分离的毛细管腔。 该方法和装置具有应用，例如，用稀释剂稀释精确量的液体。

    METHODS FOR INOCULATING CULTURE MEDIA ON PETRI DISHES BY MEANS OF VIBRATION FREQUENCIES
    65.
    发明申请
    METHODS FOR INOCULATING CULTURE MEDIA ON PETRI DISHES BY MEANS OF VIBRATION FREQUENCIES 有权
    通过振动频率对培养基培养培养基的方法

    公开(公告)号：US20140220616A1

    公开(公告)日：2014-08-07

    申请号：US14238673

    申请日：2012-08-30

    Applicant: Bruno Colin ,  Marie-Pierre Montet

    Inventor： Bruno Colin ,  Marie-Pierre Montet

    IPC: C12Q1/24 ,  C12M1/22

    CPC classification number: C12Q1/24 ,  C12M23/10 ,  C12M23/38 ,  C12M33/08

    Abstract: The present invention relates to a method for inoculating at least one agar culture medium, contained in a Petri dish, with at least one sample likely to contain microorganisms. The method includes the steps of: providing at least one agar culture medium contained in a Petri dish; providing the sample likely to contain microorganisms in liquid form; performing at least one deposition of the liquid sample onto the lid of the Petri dish such that the liquid sample can be transferred from the lid to the agar culture medium; and spraying the liquid sample, initially deposited onto the cover, onto the agar culture medium by vibrating the Petri dish.

    Abstract translation: 本发明涉及用至少一种可能含有微生物的样品接种培养皿中含有的至少一种琼脂培养基的方法。 该方法包括以下步骤：提供培养皿中所含的至少一种琼脂培养基; 提供样品可能含有液体形式的微生物; 将液体样品的至少一次沉积物执行到培养皿的盖上，使得液体样品可以从盖子转移到琼脂培养基中; 并通过振动培养皿将初始沉积到盖上的液体样品喷洒到琼脂培养基上。

    METHOD FOR ISOLATING A SAMPLE WELL OF A TEST CARD FOR ANALYSIS, AND RESULTING TEST CARD
    66.
    发明申请
    METHOD FOR ISOLATING A SAMPLE WELL OF A TEST CARD FOR ANALYSIS, AND RESULTING TEST CARD 有权
    用于分析测试卡的样品的分析方法和分析测试卡

    公开(公告)号：US20130273592A1

    公开(公告)日：2013-10-17

    申请号：US13879887

    申请日：2011-10-19

    Applicant: Bruno Colin ,  Cecile Paris

    Inventor： Bruno Colin ,  Cecile Paris

    IPC: C12Q1/24

    CPC classification number: B01L3/50853 ,  B01L3/502738 ,  B01L2200/0689 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0638 ,  B01L2400/0655 ,  C12Q1/24

    Abstract: The invention relates to a method for isolating at least one fluid sample well laid out in a test card for analyses, said test card including a plate (3) having at least one first main face from which is laid out at least one channel communicating with at least said well, this first face being coated with a membrane (17) provided with an adhesive (18) and which will cover said channel with a covering area (171), the method for isolation including a phase for introducing a fluid sample into at least said well and a phase for isolating said well consisting of exerting a force on at least one portion of the covering area (171) of the adhesive membrane (17) for ensuring displacement of the adhesive into an obturation area for the sample well.

    Abstract translation: 本发明涉及一种用于分离在测试卡中布置的至少一个流体样本的方法，用于分析，所述测试卡包括具有至少一个第一主面的板（3），所述板（3）布置在与 至少所述井，该第一面涂覆有具有粘合剂（18）的膜（17），并且其将覆盖所述通道并具有覆盖区域（171），所述隔离方法包括用于将流体样品引入的相 至少所述井以及用于隔离所述井的相，其包括在所述粘合膜（17）的覆盖区域（171）的至少一部分上施加力，以确保所述粘合剂移动到所述样品井的封闭区域。

    Holder for culture plates with de-nesting feature
    68.
    发明申请
    Holder for culture plates with de-nesting feature 有权
    用于具有去嵌套特征的培养皿的持有人

    公开(公告)号：US20100035338A1

    公开(公告)日：2010-02-11

    申请号：US12221755

    申请日：2008-08-05

    Applicant: Bruno Colin ,  Yaneli Iakimova ,  Kevin G. Ketsenburg ,  David M. Robbins ,  Stephane Bellet

    Inventor： Bruno Colin ,  Yaneli Iakimova ,  Kevin G. Ketsenburg ,  David M. Robbins ,  Stephane Bellet

    IPC: C12M1/00 ,  B65G61/00

    CPC classification number: C12M23/48 ,  C12M23/10 ,  G01N35/04 ,  G01N2035/042 ,  G01N2035/0425

    Abstract: A holding structure for holding a plurality of culture plates in a stacked arrangement includes a feature for de-nesting the bottom culture plate from the plate above it. The de-nesting feature is preferably a structure which urges a plate to move laterally relative to the plate above it. The de-nesting of the bottom culture plate from the adjacent culture plate facilitates withdrawal of the bottom culture plate from the base via a robotic plate transfer mechanism. The de-nesting feature can be entirely passive, such as ramps formed in the base of the holding structure.

    Abstract translation: 用于保持多个培养板的堆叠布置的保持结构包括用于将底部培养板从其上方的板上嵌套的特征。 去嵌套特征优选地是一种促使板相对于其上方的板横向移动的结构。 来自相邻培养板的底部培养板的解嵌有助于通过机器人板转移机构将底部培养板从基底中取出。 去嵌套特征可以是完全无源的，例如形成在保持结构的基部中的斜面。

    Device for implementing an analysis pack, analysis pack and method using same
    69.
    发明授权
    Device for implementing an analysis pack, analysis pack and method using same 失效
    用于实现分析包，分析包和使用其的方法的设备

    公开(公告)号：US07491497B2

    公开(公告)日：2009-02-17

    申请号：US11251758

    申请日：2005-10-18

    Applicant: Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

    Inventor： Bruno Colin ,  Jacques Dachaud ,  Marie Privat ,  Cécile Paris

    IPC: C12Q1/68 ,  G01N21/00

    CPC classification number: G01N35/1097 ,  B01L3/5025

    Abstract: A device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control integrated into the card; the card includes at least two sequential reaction systems arranged in parallel, each reaction system having at least two fluid transfer systems arranged in series. A test card and the method for implementing the device and the card are also disclosed. The device contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.

    Abstract translation: 一种用于实现测试卡的装置，其中在集成到卡中的控制的作用下执行顺序反应和流体转移操作; 卡片包括平行布置的至少两个顺序反应系统，每个反应系统具有串联布置的至少两个流体转移系统。 还公开了一种测试卡和用于实现设备和卡的方法。 该装置每个反应管线包含至少一个致动器，并且所有致动器彼此间隔一定距离，致动器优选等距。 本发明特别适用于生物应用中流体的显微操作。

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