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    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH
    63.
    发明公开
    PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH 有权
    处理对发酵液成分的分离

    公开(公告)号：EP2438036A1

    公开(公告)日：2012-04-11

    申请号：EP10784131.4

    申请日：2010-06-03

    Applicant: Genomatica, Inc.

    Inventor： CLARK, Warren ,  JAPS, Michael ,  BURK, Mark, J.

    IPC: C07C31/18 ,  C07C33/02

    CPC classification number: C07C29/76 ,  C12P7/18 ,  C07C31/207

    Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    64.
    发明公开
    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE 审中-公开
    有机体的环己酮生产

    公开(公告)号：EP2429587A1

    公开(公告)日：2012-03-21

    申请号：EP10775646.2

    申请日：2010-05-14

    Applicant: Genomatica, Inc.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J. ,  PHARKYA, Priti

    IPC: A61K48/00 ,  C07C49/00

    CPC classification number: C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1 -carboxyl-CoA hydrolase (acting on C-C bond), a 2-ketocyclohexane-l- carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxyl-CoA transferase, and a 2- ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6- ketocyclohex-1-ene-l-carboxyl-CoA hydrolase (acting on C-C bond), a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA synthetase, a 6-ketocyclohex-l-ene-l-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex- 1 -ene- 1 -carboxyl-CoA transferase, a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA reductase, a 6-ketocyclohex- 1 -ene- 1 -carboxylate decarboxylase, a 6- ketocyclohex-1 -ene- 1 -carboxylate reductase, a 2-ketocyclohexane-l-carboxyl-CoA synthetase, a 2-ketocyclohexane-l -carboxyl-CoA transferase, a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2- diol dehydrogenase, and a cyclohexane-l,2-diol dehydratase. A pathway includes a 3- oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID
    70.
    发明公开
    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID 有权
    OFFMUCONSÄUREPRODUZIERENDEN微生物赢了一半合成对苯二甲酸

    公开(公告)号：EP2462221A1

    公开(公告)日：2012-06-13

    申请号：EP10807185.3

    申请日：2010-08-05

    Applicant: Genomatica, Inc.

    Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

    IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

    CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

    Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2- fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a
    trans -3-hydroxy-4- hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a
    trans -3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a
    cis -3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a
    cis -3-amino-4-hexendioate deaminase, and a muconate
    cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

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