公开(公告)号：KR1020090081234A

公开(公告)日：2009-07-28

申请号：KR1020080007205

申请日：2008-01-23

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미경 ,  샤르마사이렌드라나쓰

IPC: C12N15/12 ,  C12N15/10 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/158

Abstract: A biomarker for identifying the exposure of ethylbenzene and identifying method are provided to determing the monitoring and harmfulness of ethylbenzene using the biomarker. A method for detecting the exposure of ethylbenzene using an biomarker comprises: a step of isolating RNAs from a test sample-derived human somatic cell and somatice cell of control group; a step of synthesizing cDNAs from the experimental group and control group and labeling with different fluorescence; a step of hybridizing with DNA microarray chip; and a step of analyzing DNA microarray chip.

Abstract translation: 提供了用于鉴定乙苯暴露的生物标志物和鉴定方法，以确定使用生物标志物的乙苯的监测和有害性。 使用生物标志物检测乙苯暴露的方法包括：从对照组的来自测试样品的人体细胞和体细胞中分离RNA的步骤; 从实验组和对照组合成cDNA并用不同荧光标记的步骤; 与DNA微阵列芯片杂交的步骤; 并分析DNA微阵列芯片的步骤。

公开(公告)号：KR1020090080679A

公开(公告)日：2009-07-27

申请号：KR1020080006573

申请日：2008-01-22

Applicant: 한국과학기술연구원

Inventor： 류재천 ,  김연정 ,  송미경 ,  샤르마사이렌드라나쓰

IPC: C12N15/12 ,  C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/09 ,  C12Q1/6888 ,  C12Q2600/158

Abstract: A biomarker for identifying the exposure of trichloroethylene is provided to detect trichloroethylene using genes of which expressions are overexpressed or underexpression by the trichloroethylene. A DNA microarray chip for identifying the exposure to a trichloroethylene is aggregate of a molecule containing oligonulcleotide having entire or a portion of biomarker gene sequence for identifying the exposure of trichloroethylene or its trichloroethylene. A method for identifying the exposure of trichloroethylene comprises: a step of isolating RNAs from human somatic cell isolated from a test sample (experimental group) and somatic cell of control group; a step of synthesizing RNAs to cDNAs and labeling the cDNAs of experimental group and control group; a step of hybridizing the cDNAs on DNA microarray chip; a step of analyzing DNA microarray chip; and a step of comparing the expression. The human somatic cell is derived from bone marrow or cell or tissue of leukemia patient.

Abstract translation: 提供用于鉴定三氯乙烯暴露的生物标志物，以检测三氯乙烯，其中使用表达被三氯乙烯过表达或低表达的基因。 用于鉴定暴露于三氯乙烯的DNA微阵列芯片是含有寡核苷酸的分子的聚集体，其具有用于鉴定三氯乙烯或其三氯乙烯暴露的全部或部分生物标志物基因序列。 用于鉴定三氯乙烯暴露的方法包括：从测试样品（实验组）和对照组体细胞分离的人体细胞中分离RNA的步骤; 将cDNA合成cDNA的步骤，并对实验组和对照组的cDNA进行标记; 在DNA微阵列芯片上杂交cDNAs的步骤; 分析DNA微阵列芯片的步骤; 以及比较表达的步骤。 人体细胞源自白血病患者的骨髓或细胞或组织。