公开(公告)号：KR100462287B1

公开(公告)日：2004-12-17

申请号：KR1020020012980

申请日：2002-03-11

Applicant: 한국과학기술연구원

Inventor： 정봉철 ,  정병화

IPC: G01N33/82

Abstract: PURPOSE: Provided is a method of detecting vitamin E in the serum which extracts the vitamin E from the serum, and provides optimum analyzing conditions with selective and excellent sensitivity to the high performance liquid chromatography and ultraviolet ray absorber. CONSTITUTION: A method of detecting the vitamin E in the serum comprises the steps of: treating the serum by a buffer solution containing 0.075-0.2M of salt with pH4.0-5.5; extracting the vitamin E from the treated serum by diethyl ether; and analyzing the concentration of the extracted vitamin E by a high performance liquid chromatography with a ultraviolet ray absorber. The serum is derived from livestock, the buffer solution from phosphoric acid or acetic acid, and the salt from NaCl.

Abstract translation: 目的：提供一种从血清中提取维生素E的血清中检测维生素E的方法，并且提供了对高效液相色谱和紫外线吸收剂具有选择性和优异灵敏度的最佳分析条件。 构成：检测血清中维生素E的方法包括以下步骤：用含有0.075-0.2M盐，pH4.0-5.5的缓冲溶液处理血清; 用乙醚从处理过的血清中提取维生素E; 并用紫外线吸收剂通过高效液相色谱分析提取的维生素E的浓度。 血清来源于家畜，磷酸或乙酸的缓冲溶液，以及来自NaCl的盐。

公开(公告)号：KR1020040063435A

公开(公告)日：2004-07-14

申请号：KR1020030000848

申请日：2003-01-07

Applicant: 한국과학기술연구원

Inventor： 정봉철 ,  정병화

IPC: G01N30/14

Abstract: PURPOSE: Provided are a method and an apparatus for detecting atenolol in the blood plasma by high performance liquid chromatography(HPLC), which obtain analyzing sensitivity and have accuracy, reproducibility, rapidity and convenience, and can be used as an index for determining a proper injection amount of the atenolol. CONSTITUTION: The method for detecting the atenolol contains the steps of; removing protein from the blood plasma by injecting the blood plasma into a protein removing column by using a phosphoric acid buffer solution as a developing solvent; flowing triethylamine in the protein removing column to prevent the phosphoric acid buffer solution from being flowed into a next concentration column; concentrating the protein-removed blood plasma in the concentration column, flowing triethylamine as a developing solvent. And the apparatus for detecting the atenolol contains: a developing solvent supply part containing a phosphoric acid buffer solution supply part, a triethylamine supply part, and an acetonitrile supply part; a developing solvent switching valve; the protein removing column; the concentration column; an analyzing column; a column switching valve controlling the inlet and outlet to the concentration column.

Abstract translation: 目的：提供一种通过高效液相色谱（HPLC）检测血浆中阿替洛尔的方法和装置，其获得分析灵敏度并具有准确性，重现性，快速性和便利性，并且可以用作确定适当的指标 阿替洛尔的注射量。 构成：检测阿替洛尔的方法包括以下步骤： 通过使用磷酸缓冲溶液作为显影溶剂将血浆注入蛋白质去除柱中，从血浆中除去蛋白质; 在蛋白质去除柱中流动三乙胺以防止磷酸缓冲溶液流入下一浓度柱; 将蛋白质去除的血浆浓缩在浓缩柱中，使三乙胺作为展开溶剂。 检测阿替洛尔的装置包含：含有磷酸缓冲溶液供给部的显影剂供给部，三乙胺供给部和乙腈供给部; 开发溶剂切换阀; 蛋白质去除柱; 浓度柱; 分析柱; 控制浓缩塔的入口和出口的塔切换阀。