公开(公告)号：US20140106397A1

公开(公告)日：2014-04-17

申请号：US14122373

申请日：2012-06-26

Applicant: 3M INNOVATIVE PROPERTIES COMPANY

Inventor： Raj Rajagopal ,  Kurt J. Halverson ,  Ramasubramani Kuduva Raman Thanumoorthy

IPC: C12Q1/04

CPC classification number: G01N1/4077 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2400/0409 ,  C12Q1/04 ,  G01N15/042 ,  G01N2001/4083 ,  G01N2001/4088

Abstract: Systems and methods for detecting an analyte of interest in a sample. The system can include a first container comprising a filter portion. The filter portion can include a filter comprising a filtrand of the sample on a first side of the filter. The system can further include a second container comprising the filter portion coupled to a detection portion comprising a microstructured surface. The method can include providing the first container, coupling the filter portion to the detection portion to form the second container where the first side of the filter faces the microstructured surface, and centrifuging the second container toward the microstructured surface. The method can further include inverting the second container after centrifuging to decant the supernatant from the detection portion, such that a concentrate comprising a sediment of the sample is retained in the microstructured surface, which can be interrogated for an analyte of interest.

Abstract translation: 用于检测样品中感兴趣的分析物的系统和方法。 该系统可以包括包括过滤器部分的第一容器。 过滤器部分可以包括在过滤器的第一侧上包括样品的过滤器的过滤器。 该系统还可以包括第二容器，其包括耦合到包括微结构化表面的检测部分的过滤器部分。 该方法可以包括提供第一容器，将过滤部分连接到检测部分，以形成第二容器，其中过滤器的第一侧面向微结构化表面，并将第二容器离心到微结构化表面。 该方法还可包括在离心后将第二容器倒置以从检测部分倾析上清液，使得包含样品沉淀物的浓缩物保留在微结构化表面中，该微结构化表面可被询问用于感兴趣的分析物。