公开(公告)号：EP2424975A2

公开(公告)日：2012-03-07

申请号：EP10770464.5

申请日：2010-04-30

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/21 ,  C12P7/18

CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

公开(公告)号：EP2373781A1

公开(公告)日：2011-10-12

申请号：EP09833782.7

申请日：2009-07-15

Applicant: Genomatica, Inc.

Inventor： TRAWICK, John, D. ,  BURK, Mark, J. ,  BURGARD, Anthony, P.

IPC: C12N1/00 ,  C12N1/21 ,  C12P5/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/42

Abstract: An exemplary non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol and includes a succinyl-CoA:3-ketoacid-CoA transferase. Another organism having a 4-hydroxybutryate pathway includes at least one exogenous nucleic acid including an acetoacetyl-CoA thiolase, a 3-hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA transferase, a phosphotrans-4-hydroxybutyrylase, and a 4-hydroxybutyrate kinase. An exemplary organism having a 1,4-butanediol pathway includes an acetoacetyl-CoA thiolase, a 3-Hydroxybutyryl-CoA dehydrogenase, a crotonase, a crotonyl-CoA hydratase, a 4-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA reductase (aldehyde forming), and a 1,4-butanediol dehydrogenase. Such an organism further comprising an acetyl-CoA pathway. Any of the aforementioned organisms are cultured to produce isopropanol, 4-hydroxybutryate, or 1,4- butanediol.

公开(公告)号：EP2188243A2

公开(公告)日：2010-05-26

申请号：EP08835789.2

申请日：2008-08-08

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  PHARKYA, Priti ,  VAN DIEN, Stephen, J. ,  BURGARD, Anthony, P. ,  SCHILLING, Christopher, H.

IPC: C07C51/347 ,  C07C67/333 ,  C12P7/46 ,  C07C57/04 ,  C07C69/54

CPC classification number: C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

Abstract translation: 本发明涉及烯烃的制造方法。 所述方法包括以下步骤：（a）通过发酵培养足够量的营养物和培养基产生第一种烯烃的微生物，和（b）使所述第一种烯烃与足够量的二取代的烯烃在 烯烃易位转化催化剂生产第二种不同的烯烃。 优选地，第一种烯烃是富马酸或富马酸二酯，第二种烯烃是丙烯酸或丙烯酸酯。

公开(公告)号：EP2185708A1

公开(公告)日：2010-05-19

申请号：EP08782639.2

申请日：2008-08-06

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  VAN DIEN, Stephen, J. ,  BURK, Mark, J.

IPC: C12N15/52 ,  C12P7/18 ,  C12N1/00

CPC classification number: C12P7/18 ,  B01D3/002 ,  C12N9/0006

Abstract: The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.