公开(公告)号：EP2670852A1

公开(公告)日：2013-12-11

申请号：EP12742132.9

申请日：2012-02-02

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  SUN, Jun ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12P5/02

CPC classification number: C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：EP2529011A1

公开(公告)日：2012-12-05

申请号：EP11737470.2

申请日：2011-01-21

Applicant: Genomatica, Inc.

Inventor： OSTERHOUT, Robin, E.

IPC: C12N9/00

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/88 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/62 ,  C12P9/00 ,  C12Y101/01025 ,  C12Y101/01267 ,  C12Y202/01007 ,  C12Y205/01019 ,  C12Y205/01054 ,  C12Y207/01071 ,  C12Y401/03 ,  C12Y401/0304 ,  C12Y402/01 ,  C12Y402/0101 ,  C12Y402/03004 ,  C12Y402/03005

Abstract: The invention provides non-naturally occurring microbial organisms having a (2-hydroxy- 3-methyl-4-oxobutoxy)phosphonate pathway, p- toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3- methyl-4-oxobutoxy)phosphonate pathway, p- toluate pathway or terephthalate pathway.

公开(公告)号：EP2438178A2

公开(公告)日：2012-04-11

申请号：EP10784212.2

申请日：2010-06-04

Applicant: Genomatica, Inc.

Inventor： VAN DIEN, Stephen, J. ,  BURGARD, Anthony, P. ,  HASELBECK, Robert ,  PUJOL-BAXLEY, Catherine, J. ,  NIU, Wei ,  TRAWICK, John, D. ,  YIM, Harry ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12P7/16

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：EP2429587A1

公开(公告)日：2012-03-21

申请号：EP10775646.2

申请日：2010-05-14

Applicant: Genomatica, Inc.

Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J. ,  PHARKYA, Priti

IPC: A61K48/00 ,  C07C49/00

CPC classification number: C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1 -carboxyl-CoA hydrolase (acting on C-C bond), a 2-ketocyclohexane-l- carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxyl-CoA transferase, and a 2- ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6- ketocyclohex-1-ene-l-carboxyl-CoA hydrolase (acting on C-C bond), a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA synthetase, a 6-ketocyclohex-l-ene-l-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex- 1 -ene- 1 -carboxyl-CoA transferase, a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA reductase, a 6-ketocyclohex- 1 -ene- 1 -carboxylate decarboxylase, a 6- ketocyclohex-1 -ene- 1 -carboxylate reductase, a 2-ketocyclohexane-l-carboxyl-CoA synthetase, a 2-ketocyclohexane-l -carboxyl-CoA transferase, a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2- diol dehydrogenase, and a cyclohexane-l,2-diol dehydratase. A pathway includes a 3- oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：EP2566969B1

公开(公告)日：2019-09-04

申请号：EP11778232.6

申请日：2011-05-04

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  SUN, Jun ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12P5/02

公开(公告)号：EP2744906B1

公开(公告)日：2019-05-22

申请号：EP12826412.4

申请日：2012-08-17

Applicant: Genomatica, Inc.

Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  BURK, Mark, J.

IPC: C12N15/70 ,  C12P7/18 ,  C12P7/04 ,  C12P7/16 ,  C12P7/40 ,  C12P5/02

公开(公告)号：EP2334800B1

公开(公告)日：2018-12-26

申请号：EP09813563.5

申请日：2009-09-09

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18

CPC classification number: C12P7/18 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/12 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12Y101/01157 ,  C12Y402/01055

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：EP3351619A1

公开(公告)日：2018-07-25

申请号：EP17195557.8

申请日：2009-03-27

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  PHARKYA, Priti ,  OSTERHOUT, Robin, E.

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：EP2424975B1

公开(公告)日：2016-07-06

申请号：EP10770464.5

申请日：2010-04-30

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/21 ,  C12P7/18 ,  C12N9/04

CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

公开(公告)号：EP2931874A1

公开(公告)日：2015-10-21

申请号：EP13865674.9

申请日：2013-12-16

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  VAN DIEN, Stephen, J. ,  TRACEWELL, Cara, Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC: C12N1/21

CPC classification number: C12P17/10 ,  A23L29/065 ,  C08G63/78 ,  C08G69/08 ,  C08G69/16 ,  C08G73/0213 ,  C12N9/0006 ,  C12N15/52 ,  C12P7/24 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01244

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.