公开(公告)号：WO03014362A3

公开(公告)日：2003-05-01

申请号：PCT/EP0208231

申请日：2002-07-24

Applicant: BASF AG ,  POMPEJUS MARKUS ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  LIEBL WOLFGANG

Inventor： POMPEJUS MARKUS ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  LIEBL WOLFGANG

IPC: C12N15/09 ,  C12N1/21 ,  C12N15/54 ,  C12N15/77

CPC classification number: C12N15/77

Abstract: The invention relates to a plasmid vector which does not replicate in a target organism and contains the following constituents: a) an origin of replication for a host organism which is not identical to the target organism, b) at least one genetic marker, c) optionally a section of a sequence which enables the transfer of DNA by means of conjugation (mob sequence), d) a section of a sequence which is homologous to sequences of the target organism and enables homologous recombination in the target organism, e) a gene for a galactokinase under the control of a promoter.

Abstract translation: 本发明涉及不在目标生物体中复制，包括以下组分的质粒载体：对于宿主生物体，其是不相同的目标生物体，b）至少一个遗传标记，c）任选的序列部分中的）复制起点（复制起点） 该DNA的通过接合转移允许（MOB序列），D）的序列部分，其是同源的靶生物的序列，并允许靶生物体的同源重组，e）在一个启动子的控制下的半乳糖激酶的基因。