公开(公告)号：WO1998027463A1

公开(公告)日：1998-06-25

申请号：PCT/US1997023714

申请日：1997-12-17

Applicant: EVERHART, Dennis, S. ,  WHITESIDES, George, M.

IPC: G03F07/16

CPC classification number: G03F7/0002 ,  B05D1/283 ,  B41M3/003 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  G03F7/161 ,  G03F7/165 ,  Y10T428/249983 ,  Y10T428/2804 ,  Y10T428/31703

Abstract: The present invention comprises methods of contact printing of patterned, self-assembling monolayers of alkanethiolates, carboxylic acids, hydroxamic acids, and phosphonic acids on metallized thermoplastic films, the compositions produced thereby, and the use of these compositions. Patterned self-assembling monolayers allow for the controlled placement of fluids thereon which contain a chemically reactive, indicator functionality. The optical sensing devices produced thereby when the film is exposed to an analyte and light, can produce optical diffraction patterns which differ depending on the reaction of the self-assembling monolayer with the analyte of interest. The light can be in the visible spectrum, and be either reflected from the film, or transmitted through it, and the analyte can be any compound reacting with the fluid on the self-assembling monolayer. The present invention also provides a flexible support for a self-assembling monolayer on gold or another suitable metal.

Abstract translation: 本发明包括在金属化热塑性薄膜上接触印刷图案化的自组装单烷基硫醇盐，羧酸，异羟肟酸和膦酸的单元，由此制备的组合物以及这些组合物的用途。 图案化的自组装单层允许在其上控制放置含有化学反应性指示剂功能的流体。 当膜暴露于分析物和光时由此产生的光学感测装置可以产生根据自组装单层与感兴趣的分析物的反应而不同的光学衍射图。 光可以在可见光谱中，并且可以从膜反射或透过它，并且分析物可以是与自组装单层上的流体反应的任何化合物。 本发明还提供了一种对金或其它合适金属上的自组装单层的柔性支撑。

公开(公告)号：WO1997007429A1

公开(公告)日：1997-02-27

申请号：PCT/US1996013223

申请日：1996-08-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  CLEM, Paul, G. ,  JEON, Noo-Li ,  MRKSICH, Milan ,  NUZZO, Ralph, G. ,  PAYNE, David, A. ,  WHITESIDES, George, M. ,  XIA, Younan

Inventor： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

IPC: G03F07/00

CPC classification number: B82Y30/00 ,  B01J2219/00317 ,  B01J2219/00382 ,  B01J2219/00659 ,  B05D1/283 ,  B41M3/003 ,  B82Y10/00 ,  B82Y40/00 ,  C23C16/01 ,  C23C16/04 ,  C23C16/042 ,  C40B60/14 ,  G03F7/0002 ,  H01L21/28562 ,  H01L21/76879 ,  H01L21/76898

Abstract: A technique for creating patterns of material deposited on a surface involves forming a self-assembled monolayer in a pattern on the surface and depositing, via chemical vapor deposition or via sol-gel processing, a material on the surface in a pattern complementary to the self-assembled monolayer pattern. The material can be a metal, metal oxide, or the like. The surface can be contoured, including trenches or holes, the trenches or holes remaining free of self-assembled monolayer while the remainder of the surface is coated. When exposed to deposition conditions, metal or metal oxide is deposited in the trenches or holes, and remaining portions of the article surface remain free of deposition. The technique finds particular use in creation of conductive metal pathways selectively within holes passing from one side of a substrate to another.

Abstract translation: 用于产生沉积在表面上的材料图案的技术包括以表面上的图案形成自组装单层，并通过化学气相沉积或经由溶胶 - 凝胶加工，以与自身互补的图案沉积在表面上的材料 组装的单层图案。 该材料可以是金属，金属氧化物等。 表面可以是轮廓，包括沟槽或孔，沟槽或孔保持没有自组装单层，而表面的其余部分被涂覆。 当暴露于沉积条件时，金属或金属氧化物沉积在沟槽或孔中，并且制品表面的剩余部分保持不沉积。 该技术特别用于在从衬底的一侧通过到另一侧的孔内选择性地形成导电金属通路。

公开(公告)号：WO1983003846A1

公开(公告)日：1983-11-10

申请号：PCT/US1982000534

申请日：1982-04-23

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FIRMENICH, S.A. ,  WHITESIDES, George, M. ,  MAZENOD, François, P. ,  WONG, Chi-Wuei

Inventor： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  FIRMENICH, S.A.

IPC: C12P19/24

CPC classification number: C07H3/08 ,  C12P19/24 ,  C12P19/26

Abstract: 6-Deoxy-D-fructose and 6-deoxy-L-sorbose, useful starting materials for the synthesis of 4-hydroxy-2,5dimethyl-2,3-dihydrofuran-3-one, are obtained from fructose-1,6-diphosphate by a reaction with lactaldehyde, in the presence of an enzymatic system composed of aldolase and triose phosphate isomerase (TPI) in an aqueous medium at pH 7.0, followed by the hydrolysis of the monophosphate salt thus obtained. The same deoxy-sugars are obtained by the reaction between 1,3-dihydroxyacetone phosphate and lactaldehyde in the presence of the same enzymatic system composed of aldolase and TPI. The aforementioned deoxy-sugars are also prepared by the reaction of 1,3-dihydroxy-acetone with lactaldehyde in the presence of an anionic exchange resin.

Abstract translation: 6-脱氧-D-果糖和6-脱氧-L-山梨糖是用于合成4-羟基-2,5-二甲基-2,3-二氢呋喃-3-酮的起始原料，由果糖-1,6-二 在pH 7.0的水性介质中，在由醛缩酶和丙糖磷酸异构酶（TPI）组成的酶系统的存在下，通过与乳醛的反应，然后水解由此得到的单磷酸盐，从而与二羧酸反应。 在由醛缩酶和TPI组成的相同酶系统的存在下，通过1,3-二羟基丙酮磷酸酯和内酰胺之间的反应获得相同的脱氧糖。 上述脱氧糖还可以通过在阴离子交换树脂的存在下通过1,3-二羟基 - 丙酮与内酰胺的反应来制备。

公开(公告)号：WO2023049854A1

公开(公告)日：2023-03-30

申请号：PCT/US2022/076956

申请日：2022-09-23

Applicant: SAKAMOTO, Junji ,  TEN, Alexei ,  KODAIMATI, Mohamad ,  JIANG, Haihui, Joy ,  NAGARKAR, Amit, Arun ,  ROOT, Samuel, E. ,  WHITESIDES, George, M.

Inventor： SAKAMOTO, Junji ,  TEN, Alexei ,  KODAIMATI, Mohamad ,  JIANG, Haihui, Joy ,  NAGARKAR, Amit, Arun ,  ROOT, Samuel, E. ,  WHITESIDES, George, M.

IPC: C01B25/41 ,  A61L27/52 ,  C01B25/34

Abstract: A polyphosphate material is disclosed. The polyphosphate material can include a plurality of polyphosphate chains. The polyphosphate chains can have a backbone that include oxygen-phosphate bonds. Two or more cations can be included. Further, the polyphosphate material can be amorphous. The two or more cations can be monovalent cations, divalent cations, trivalent cations, tetravalent cations, and combinations thereof. The two or more cations can be lithium, sodium, potassium, rubidium, cesium, francium, ammonium, beryllium, magnesium, calcium, strontium, barium, radium, zinc, titanium, iron (Fe2+), chromium (Cr2+), manganese (Mn2+), cobalt (Co2+), nickel (Ni2+), copper (Cu2+), cadmium, tin (Sn2+), mercury (Hg2+), lead (Pb2+), aluminum, boron, gallium, iron (Fe+3), chromium (Cr+3), cobalt (Co+3), gold (Au+3), antimony (Sb+3), nickel (Ni+3), bismuth (Bi+3), manganese (Mn+3), zirconium, silicon, and combinations of thereof. The two or more cations can be monovalent cations. The two or more cations can be sodium and potassium or potassium and lithium.

公开(公告)号：WO2018053108A1

公开(公告)日：2018-03-22

申请号：PCT/US2017/051533

申请日：2017-09-14

Applicant: ÜNAL, Baris ,  HAMEDI, Mahiar, Max ,  KERR, Emily ,  GLAVAN, Ana, C. ,  FERNANDEZ-ABEDUL, Maria, Teresa ,  WHITESIDES, George, M.

Inventor： ÜNAL, Baris ,  HAMEDI, Mahiar, Max ,  KERR, Emily ,  GLAVAN, Ana, C. ,  FERNANDEZ-ABEDUL, Maria, Teresa ,  WHITESIDES, George, M.

IPC: B01L3/00 ,  B29C59/00 ,  B29C59/02

Abstract: Embodiments of the invention provide cellophane based microplates and microfluidic devices. Under one aspect, a microplate includes a plate; and an array of wells formed in the plate, wherein the plate comprises cellophane. Under another aspect, a microfluidic device includes at least one sheet of cellophane; a second sheet of material sealed to the sheet of cellophane; a channel formed between the at least one sheet of cellophane and the second sheet; and at least a first liquid inlet in communication with the channel.

Abstract translation: 本发明的实施例提供了玻璃纸基微孔板和微流体装置。 在一个方面，微板包括板; 以及在板中形成的孔阵列，其中板包含玻璃纸。 在另一方面，微流体装置包括至少一片玻璃纸; 将第二片材料密封到玻璃纸片上; 在所述至少一片玻璃纸片和所述第二片之间形成的通道; 和至少一个与通道连通的第一液体入口。

公开(公告)号：WO2017024297A1

公开(公告)日：2017-02-09

申请号：PCT/US2016/045977

申请日：2016-08-08

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  MILETTE, Jonathan ,  WHITESIDES, George, M. ,  CHRISTODOULEAS, Dionysios ,  SMITH, Barbara, S. ,  MAXWELL, Elizabeth, Jane ,  BADU-TAWIAH, Abraham, Kwame ,  NOXON, Isabelle ,  TSALOGLOU, Maria-Nefeli ,  VERSA, Mohit ,  KENDAL, Miley ,  IM, Judy

Inventor： MILETTE, Jonathan ,  WHITESIDES, George, M. ,  CHRISTODOULEAS, Dionysios ,  SMITH, Barbara, S. ,  MAXWELL, Elizabeth, Jane ,  BADU-TAWIAH, Abraham, Kwame ,  NOXON, Isabelle ,  TSALOGLOU, Maria-Nefeli ,  VERSA, Mohit ,  KENDAL, Miley ,  IM, Judy

IPC: G01N33/53 ,  G01N33/52 ,  G01N33/561 ,  B81B1/00 ,  B01L3/00 ,  B01L99/00

CPC classification number: B01L3/502707 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/126 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0633 ,  G01N33/56988 ,  G01N33/56994 ,  G01N33/571

Abstract: A three-dimensional microfluidic device is described, including: a plurality of porous, hydrophilic cellulosic layers each comprising one or more hydrophilic regions and/or hydrophilic channels embedded in the porous, hydrophilic cellulosic layer; wherein the hydrophilic channel is fluidically connected to the hydrophilic region; the hydrophilic regions comprise a sample deposition zone, one or more assay zones in fluidic communication with the sample deposition zone; and a buffer deposition zone; and the assay zone comprises one or more reagents embedded therein selected from the group consisting of antigens and antibodies; and a valve layer comprising a valve switchable from a first position where the buffer zone and the assay zone are not in fluidic communication, to a second position where the buffer zone and the assay zone are in fluidic communication.

Abstract translation: 描述了一种三维微流体装置，其包括：多个多孔的亲水性纤维素层，其各自包含一个或多个亲水性区域和/或亲水通道，该亲水区域和/或亲水通道嵌入多孔亲水性纤维素层中; 其中所述亲水通道与所述亲水区流体连接; 亲水区域包括样品沉积区，与样品沉积区流体连通的一个或多个测定区; 和缓冲沉积区; 并且所述测定区包含一种或多种嵌入其中的选自抗原和抗体的试剂; 以及阀层，其包括可从所述缓冲区和所述测定区未流体连通的第一位置切换到所述缓冲区和所述测定区处于流体连通的第二位置的阀。

公开(公告)号：WO2012162345A1

公开(公告)日：2012-11-29

申请号：PCT/US2012/039058

申请日：2012-05-23

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  WHITESIDES, George M. ,  DERDA, Ratmir ,  FULLER, Renee ,  DEISS, Frederique T.

Inventor： WHITESIDES, George M. ,  DERDA, Ratmir ,  FULLER, Renee ,  DEISS, Frederique T.

IPC: G01N33/483 ,  C12N5/08

CPC classification number: G01N33/4833 ,  G01N33/5091 ,  G01N33/574

Abstract: The present invention relates to methods, compositions and kits related to a novel in vitro assay for a high-capacity and high-throughput method for measuring the ability of cancer cells to migrate in a three-dimensional cellular assay. The three-dimensional cellular invasion assay provides a method for determining and quantitating the metastatic potential and invasive capacity of a cancer cell. Other aspects of the invention further relate to the use of the in vitro assay to screen for agents and compounds capable of inhibiting intravasation, and thereby modulating the metastatic potential of cancer cells. The methods, compositions and three-dimensional assay provide a highly sensitive assay system capable of mimicking the in vivo cellular and molecular interactions required for successful completion of intravasation.

Abstract translation: 本发明涉及与用于测量癌细胞在三维细胞测定中迁移的能力的高容量和高通量方法的新体外测定相关的方法，组合物和试剂盒。 三维细胞侵袭测定提供了确定和定量癌细胞转移潜能和侵袭能力的方法。 本发明的其它方面进一步涉及使用体外测定来筛选能够抑制渗透的药剂和化合物，从而调节癌细胞的转移潜力。 方法，组合物和三维测定提供了能够模拟成功完成渗透所需的体内细胞和分子相互作用的高度灵敏的测定系统。

公开(公告)号：WO2012148472A2

公开(公告)日：2012-11-01

申请号：PCT/US2011/061720

申请日：2011-11-21

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  ILIEVSKI, Filip ,  CHEN, Xin ,  MAZZEO, Aaron, D. ,  WHITESIDES, George, M. ,  SHEPHERD, Robert ,  MARTINEZ, Ramses ,  CHOI, Won, Jae ,  KWOK, Sen, W. ,  MORIN, Stephen ,  STOKES, Adam ,  NIE, Zhihong

Inventor： ILIEVSKI, Filip ,  CHEN, Xin ,  MAZZEO, Aaron, D. ,  WHITESIDES, George, M. ,  SHEPHERD, Robert ,  MARTINEZ, Ramses ,  CHOI, Won, Jae ,  KWOK, Sen, W. ,  MORIN, Stephen ,  STOKES, Adam ,  NIE, Zhihong

IPC: F41H3/00

CPC classification number: F15B15/10 ,  B25J9/1075 ,  B25J9/142 ,  B25J11/00 ,  F15B7/06

Abstract: A soft robotic device includes a flexible body having a width, a length and a thickness, wherein the thickness is at least 1 mm, the flexible body having at least one channel disposed within the flexible body, the channel defined by upper, lower and side walls, wherein at least one wall is strain limiting; and a pressurizing inlet in fluid communication with the at least one channel, the at least one channel positioned and arranged such that the wall opposite the strain limiting wall preferentially expands when the soft robotic device is pressurized through the inlet.

Abstract translation: 软机器人装置包括具有宽度，长度和厚度的柔性体，其中所述厚度为至少1mm，所述柔性体具有设置在所述柔性体内的至少一个通道，所述通道由上部，下部和侧面限定 壁，其中至少一个壁是应变限制; 以及与所述至少一个通道流体连通的加压入口，所述至少一个通道定位和布置成使得当所述软自动装置通过所述入口被加压时，与所述应变限制壁相对的壁优先地膨胀。

公开(公告)号：WO2012047324A3

公开(公告)日：2012-09-07

申请号：PCT/US2011039932

申请日：2011-06-10

Applicant: HARVARD COLLEGE ,  DERDA RATMIR ,  TANG SINDY K Y ,  WHITESIDES GEORGE M

Inventor： DERDA RATMIR ,  TANG SINDY K Y ,  WHITESIDES GEORGE M

IPC: C12N15/10 ,  C40B40/02 ,  C40B50/06

CPC classification number: C12N15/1037 ,  C40B40/02 ,  C40B50/06

Abstract: The present invention generally relates to amplification of biological entities, for example, for phage display. In one aspect, members of a library of biological entities are encapsulated in separate compartments (e.g., in separate microfluidic droplets) and amplified. As a specific example, by putting members of a phage display library into microfluidic droplets such that no droplet contains more than one member of the library, the library can be amplified without any substantial changes in growth rates or population distributions, or other artifacts created due to differences in growth rates or amplification between different members of the library. In some cases, the volume of the compartments can be used to control the copy number of a biological entity during amplification. In certain cases, biological entities with different amplification rates can be amplified independently of each other. In some embodiments, the ratio of a rapidly amplifying biological entity to a slowly amplifying biological entity can be controlled. This can be advantageous, for example, in preserving diversity within a library by preventing rapidly amplifying biological entities from outcompeting slowly amplifying biological entities. For example, certain methods and systems of the invention can be useful in situations where preferential amplification of library members can present a problem.

Abstract translation: 本发明通常涉及生物实体的扩增，例如用于噬菌体展示。 在一个方面，生物实体文库的成员被包封在分离的隔室中（例如，在单独的微流体液滴中）并被扩增。 作为具体实例，通过将噬菌体展示文库的成员置于微流体液滴中，使得没有液滴含有多于一个文库的成员，可以扩增文库，而不增加生长速率或群体分布或由其产生的其他工件 图书馆不同成员之间的增长速度差异或差异。 在一些情况下，隔室的体积可以用于在放大期间控制生物实体的拷贝数。 在某些情况下，具有不同扩增率的生物实体可以彼此独立地扩增。 在一些实施方案中，可以控制快速扩增的生物实体与缓慢扩增的生物实体的比例。 这可能是有利的，例如，通过防止快速扩增生物实体从超竞争的缓慢扩大的生物实体来保存文库内的多样性。 例如，本发明的某些方法和系统可用于库成员的优先扩增可能存在问题的情况。

公开(公告)号：WO2012047324A2

公开(公告)日：2012-04-12

申请号：PCT/US2011/039932

申请日：2011-06-10

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  DERDA, Ratmir ,  TANG, Sindy K.Y. ,  WHITESIDES, George M.

Inventor： DERDA, Ratmir ,  TANG, Sindy K.Y. ,  WHITESIDES, George M.

IPC: C12N15/10

CPC classification number: C12N15/1037 ,  C40B40/02 ,  C40B50/06

Abstract: The present invention generally relates to amplification of biological entities, for example, for phage display. In one aspect, members of a library of biological entities are encapsulated in separate compartments (e.g., in separate microfluidic droplets) and amplified. As a specific example, by putting members of a phage display library into microfluidic droplets such that no droplet contains more than one member of the library, the library can be amplified without any substantial changes in growth rates or population distributions, or other artifacts created due to differences in growth rates or amplification between different members of the library. In some cases, the volume of the compartments can be used to control the copy number of a biological entity during amplification. In certain cases, biological entities with different amplification rates can be amplified independently of each other. In some embodiments, the ratio of a rapidly amplifying biological entity to a slowly amplifying biological entity can be controlled. This can be advantageous, for example, in preserving diversity within a library by preventing rapidly amplifying biological entities from outcompeting slowly amplifying biological entities. For example, certain methods and systems of the invention can be useful in situations where preferential amplification of library members can present a problem.

Abstract translation: 本发明一般涉及生物实体的扩增，例如用于噬菌体展示。 一方面，生物实体文库的成员被封装在单独的隔室中（例如，在分开的微流体液滴中）并被扩增。 作为一个具体的例子，通过将噬菌体展示文库的成员放入微流体微滴中，使得没有微滴包含超过一个的文库成员，可以扩增该文库而没有任何实质性的生长速率或种群分布变化，或者其他产生的文物 图书馆不同成员之间的增长率或放大率差异。 在一些情况下，隔室的体积可用于在扩增期间控制生物实体的拷贝数。 在某些情况下，具有不同扩增速率的生物实体可以彼此独立放大。 在一些实施方案中，可以控制快速放大生物实体与缓慢放大生物实体的比率。 例如，这通过防止快速扩增的生物实体失去缓慢放大的生物实体来保持文库内的多样性可能是有利的。 例如，本发明的某些方法和系统在文库成员的优先扩增可能存在问题的情况下可能是有用的。