公开(公告)号：EP2909325A1

公开(公告)日：2015-08-26

申请号：EP13848548.7

申请日：2013-10-21

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  VAN DIEN, Stephen, J. ,  TRACEWELL, Cara, Ann ,  PHARKYA, Priti ,  ANDRAE, Stefan

IPC: C12P1/04

CPC classification number: C12P7/46 ,  C07C55/10 ,  C07C59/235 ,  C08G63/00 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/70 ,  C12N15/74 ,  C12N15/77 ,  C12N15/81 ,  C12N15/815 ,  C12P7/24 ,  C12Y101/01244 ,  C12Y105/0102

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.

公开(公告)号：EP2855687A2

公开(公告)日：2015-04-08

申请号：EP13800646.5

申请日：2013-06-03

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  PHARKYA, Priti ,  BURGARD, Anthony, P. ,  VAN DIEN, Stephen, J. ,  OSTERHOUT, Robin, E. ,  TRAWICK, John, D. ,  KUCHINSKAS, Michael, P. ,  STEER, Brian

IPC: C12P7/52 ,  C12N1/20 ,  C12N1/21

CPC classification number: C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：EP2462221A1

公开(公告)日：2012-06-13

申请号：EP10807185.3

申请日：2010-08-05

Applicant: Genomatica, Inc.

Inventor： BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2- fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a
trans -3-hydroxy-4- hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a
trans -3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a
cis -3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a
cis -3-amino-4-hexendioate deaminase, and a muconate
cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

公开(公告)号：EP2424975A2

公开(公告)日：2012-03-07

申请号：EP10770464.5

申请日：2010-04-30

Applicant: Genomatica, Inc.

Inventor： BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

IPC: C12N1/21 ,  C12P7/18

CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.