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    NON-NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGETIC EFFICIENCY
    85.
    发明公开
    NON-NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGETIC EFFICIENCY 审中-公开
    提高能量效率的非天然微生物生物

    公开(公告)号：EP3194604A1

    公开(公告)日：2017-07-26

    申请号：EP15775307.0

    申请日：2015-09-18

    Applicant: Genomatica, Inc.

    Inventor： PHARKYA, Priti ,  BURGARD, Anthony P. ,  NUNEZ VAN NAME,Eric Roland

    IPC: C12P7/42 ,  C12P7/16

    CPC classification number: C12N15/52 ,  C12N9/1205 ,  C12N9/88 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12Y207/0104 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02E50/343

    Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

    Abstract translation: 本发明提供了包含酶途径和/或代谢修饰的非天然微生物，用于通过乙酰-CoA或草酰乙酸和乙酰-CoA增强碳通量。 本发明的实施方案包括具有通向乙酰-CoA和包括磷酸酮醇酶（PK途径）的草酰乙酸的途径的微生物。 生物体还具有（i）增强非磷酸转移酶系统（非PTS）的糖摄取活性的基因修饰，和/或（ii）对生物体电子传递链（ETC）的遗传修饰 ），提高ATP生产效率，增强还原等价物的可用性或两者。 微生物有机体可以任选地包括（iii）维持，减弱或消除磷酸转移酶系统（PTS）的糖摄取活性的遗传修饰。 通过乙酰-CoA和草酰乙酸的增加的碳通量可以用于生物衍生化合物的生产，并且微生物生物体可以进一步包括能够生产生物来源化合物的途径。

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF BUTADIENE USING ACETYL-COA
    86.
    发明公开
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF BUTADIENE USING ACETYL-COA 审中-公开
    用ACETYL-COA生产丁二烯的微生物和方法

    公开(公告)号：EP3167066A1

    公开(公告)日：2017-05-17

    申请号：EP15818352.5

    申请日：2015-07-02

    Applicant: Genomatica, Inc.

    Inventor： PHARKYA, Priti ,  BURGARD, Anthony P. ,  BURK, Mark J.

    IPC: C12N15/70 ,  C12P5/02 ,  C12N15/63 ,  C12N15/03

    CPC classification number: C12P5/026 ,  C01B3/00 ,  C08F36/06 ,  C08F36/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0067 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52 ,  C12P3/00 ,  C12P7/40 ,  C12Y101/02007 ,  C12Y101/03013 ,  C12Y102/99003 ,  Y02E50/343

    Abstract: The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

    Abstract translation: 本发明提供了含有丁二烯或2,4-戊二烯酸途径的非天然存在的微生物生物体，其包含至少一种编码丁二烯或2,4-戊二烯酸途径酶的外源核酸，所述外源核酸以足以产生丁二烯或2,4-戊二烯酸 。 生物体可以进一步含有氢合成途径。 本发明另外提供了通过培养如本文所述的包含丁二烯或2,4-戊二烯酸酯途径的非天然存在的微生物有机体在一定条件下并持续足够的时间以产生丁二烯或2,4-戊二烯酸的方法来生产丁二烯或2,4-戊二烯酸的方法， 生产丁二烯或2,4-戊二烯酸。 氢可以与丁二烯或2,4-戊二烯酸的生产一起生产。

    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1.4- CYCLOHEXANEDIMETHANOL
    89.
    发明公开
    MICROORGANISMS AND METHODS FOR THE PRODUCTION OF 1.4- CYCLOHEXANEDIMETHANOL 审中-公开
    微生物及其制备方法1,4-环己烷

    公开(公告)号：EP2652141A1

    公开(公告)日：2013-10-23

    申请号：EP11848110.0

    申请日：2011-12-15

    Applicant: Genomatica, Inc.

    Inventor： PHARKYA, Priti ,  BURK, Mark, J.

    IPC: C12P7/18

    CPC classification number: C12P7/02 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12N15/76 ,  C12N15/78 ,  C12N15/81 ,  C12N15/815 ,  C12P7/18

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,4-cyclohexanedimethanol pathway that includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol. A method for producing 1,4-cyclohexanedimethanol includes culturing a non-naturally occurring microbial organism having a 1,4-cyclohexanedimethanol pathway. The pathway includes at least one exogenous nucleic acid encoding a 1,4-cyclohexanedimethanol pathway enzyme expressed in a sufficient amount to produce 1,4-cyclohexanedimethanol, under conditions and for a sufficient period of time to produce 1,4-cyclohexanedimethanol.

    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS
    90.
    发明公开
    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS 审中-公开
    微生物和方法异丙醇和伯醇，DIOLEN和酸的同时生产

    公开(公告)号：EP2475775A1

    公开(公告)日：2012-07-18

    申请号：EP10816105.0

    申请日：2010-09-09

    Applicant: Genomatica, Inc.

    Inventor： PHARKYA, Priti ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  BURK, Mark, J. ,  SUN, Jun

    IPC: C12P7/04 ,  C12N15/74

    CPC classification number: C12P7/40 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18

    Abstract: The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13- BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanolpathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.

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