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    Detection of the receptor oligomerization
    1.
    发明专利
    Detection of the receptor oligomerization 审中-公开
    检测受体低聚

    公开(公告)号：JP2009271087A

    公开(公告)日：2009-11-19

    申请号：JP2009188699

    申请日：2009-08-17

    Applicant: Aclara Biosciences Inc ,  アクララ バイオサイエンシーズ, インコーポレイテッド

    Inventor： CHAN-HUI PO-YING ,  SHI YINING ,  PIDAPARTHI SAILAJA ,  DUA RAJIV ,  SINGH SHARAT

    IPC: G01N33/53 ,  C12Q1/68 ,  G01N20060101 ,  G01N27/447 ,  G01N33/483 ,  G01N33/542 ,  G01N33/543 ,  G01N33/556 ,  G01N33/566 ,  G01N33/567 ,  G01N33/68

    CPC classification number: G01N33/566 ,  G01N33/542 ,  G01N2333/71 ,  G01N2333/726

    Abstract: PROBLEM TO BE SOLVED: To provide a method of detecting formation of oligomer complexes of molecules on surfaces of cell membranes. SOLUTION: This method employs pairs of tagged probes and cleaving probes, each of which binds specifically to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, and providing measure of formation of the complex. COPYRIGHT: (C)2010,JPO&INPIT

    Abstract translation: 待解决的问题：提供检测细胞膜表面上分子的低聚物复合物的形成的方法。 解决方案：该方法采用成对的标记探针和切割探针，每个探针和切割探针都与细胞表面分子特异性结合。 标记的探针包括通过可切割键连接到第一结合化合物的分子标签，并且所述切割探针包括第二结合剂和切割诱导部分，其在限定的接近于其内时可切割连接。 两个探针与形成低聚复合物的细胞表面分子的结合导致分子标签从结合化合物的释放，并提供复合物形成的测量。 版权所有（C）2010，JPO＆INPIT

    Microfluid device with sample injector, and method
    3.
    发明专利
    Microfluid device with sample injector, and method 审中-公开
    具有样品注射器的微流体装置和方法

    公开(公告)号：JP2008209407A

    公开(公告)日：2008-09-11

    申请号：JP2008019607

    申请日：2008-01-30

    Applicant: Aclara Biosciences Inc ,  アクララ バイオサイエンシーズ, インコーポレイテッド

    Inventor： WILLIAMS STEPHEN J ,  TAN HONG DONG ,  KAO HUNG PIN ,  VREELAND WYATT N

    IPC: G01N27/447 ,  B01D57/02 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B81B1/00 ,  C07K1/28 ,  C08F2/58 ,  G01N37/00

    CPC classification number: C07K1/28 ,  B01L3/5027 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  G01N27/44743 ,  G01N27/44747 ,  G01N27/44773 ,  G01N27/44791

    Abstract: PROBLEM TO BE SOLVED: To provide a microfluid device and a system which are controlled for attaining various desirable sample injection characteristics. SOLUTION: The system is employed for injecting a liquid sample into an electrolyte channel inside a microfluid device that has a channel network, and the channel network includes an electrolyte channel having upstream and downstream channel portions and a first side channel, a second side channel, and a third side channel. The first side channel, the second side channel and the third side channel intersect the electrolyte channel between the two channel portions respectively at a first port, a second port, and a third port; at least one port has an axial section, crossing the electrolyte channel between the two channel sections; and here, at least one of the ports has a gap spaced in the axial direction, from the other two ports along the electrolyte. COPYRIGHT: (C)2008,JPO&INPIT

    Abstract translation: 要解决的问题：提供一种微流体装置和系统，其被控制以实现各种期望的样品注射特性。 解决方案：该系统用于将液体样品注入到具有通道网络的微流体装置内的电解液通道中，并且通道网络包括具有上游和下游通道部分和第一侧通道的电解质通道，第二 侧通道和第三侧通道。 第一侧通道，第二侧通道和第三侧通道分别在第一端口，第二端口和第三端口处与两个通道部分之间的电解液通道相交; 至少一个端口具有轴向截面，与两个通道段之间的电解液通道相交; 并且这里，至少一个端口沿着沿着电解质的其它两个端口在轴向方向上间隔开间隙。 版权所有（C）2008，JPO＆INPIT

    Tandem isotachophoresis/zone electrophoresis method and system
    5.
    发明申请
    Tandem isotachophoresis/zone electrophoresis method and system 有权

    公开(公告)号：US20040060821A1

    公开(公告)日：2004-04-01

    申请号：US10676857

    申请日：2003-09-30

    Applicant: ACLARA BioSciences, Inc.

    Inventor： Stephen J. Williams ,  Hong Dong Tan ,  Hung Pin Kao ,  Wyatt N. Vreeland

    IPC: G01N027/26

    CPC classification number: B01L3/5027 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/049 ,  C07K1/26 ,  C07K1/28 ,  G01N27/44773

    Abstract: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

    Multiple array microfluidic device units
    6.
    发明申请
    Multiple array microfluidic device units 审中-公开
    多阵列微流体装置单元

    公开(公告)号：US20020092767A1

    公开(公告)日：2002-07-18

    申请号：US09995909

    申请日：2001-11-28

    Applicant: ACLARA BioSciences, Inc.

    Inventor： Torleif Ove Bjornson ,  Timothy F. Smith

    IPC: G01N027/26 ,  G01N027/447

    CPC classification number: B01L3/0241 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00358 ,  C40B60/14 ,  G01N35/1065 ,  G01N35/1074 ,  G01N2035/1037

    Abstract: Microfluidic unit arrays and their use are provided for performing in parallel a plurality of operations. The units are arrayed in a format comparable to microtiter well formats, so that transfer by a dispenser having a plurality of dispensing units can be performed with the same footprint, the format of the source and microfluidic unit receiving reservoirs are substantially the same. Operations are carried out simultaneously under comparable conditions, which permits more exact comparisons between the operations.

    Abstract translation: 微流体单元阵列及其用途被提供用于并行执行多个操作。 这些单元以与微量滴定孔格式相当的格式排列，使得具有多个分配单元的分配器的转印可以以相同的占地面积进行，源和微流体单元接收储存器的格式基本相同。 操作在可比较的条件下同时进行，这样可以更准确地比较操作。

    Dynamic coating with linear polymer mixture for electrophoresis
    7.
    发明申请
    Dynamic coating with linear polymer mixture for electrophoresis 有权
    用线性聚合物混合物动态涂布电泳

    公开(公告)号：US20020029968A1

    公开(公告)日：2002-03-14

    申请号：US09847780

    申请日：2001-05-01

    Applicant: Aclara BioSciences, Inc.

    Inventor： Hongdong Roy Tan ,  Alexander Sassi ,  Ingrid Cruzado

    IPC: G01N027/26 ,  G01N027/447

    CPC classification number: G01N27/44747 ,  G01N27/44752

    Abstract: Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in greater amount. Of particular interest are uncrosslinked acrylamide polymer mixtures for coating plastic channels and providing sieving for performing DNA separations in microfluidic devices. Polyacrylamide or N,N-dimethyl acrylamide is used with a N,N-dialkyl acrylamide copolymer, either separately or together for sieving and coating, serving as the medium in capillary electrophoresis DNA separations.

    Abstract translation: 提供了组合物和方法，用于使用组合物在包含聚合物和筛分聚合物的水性缓冲介质中组合的组合物进行毛细管电泳，其中筛分聚合物比涂覆聚合物更亲水并且以更大的量存在。 特别令人感兴趣的是用于涂覆塑料通道的未交联的丙烯酰胺聚合物混合物，并提供用于在微流体装置中进行DNA分离的筛分。 聚丙烯酰胺或N，N-二甲基丙烯酰胺与N，N-二烷基丙烯酰胺共聚物分别或一起用于筛分和包衣，用作毛细管电泳DNA分离中的培养基。

    Compositions and methods employing cleavable electrophoretic tag reagents
    10.
    发明申请
    Compositions and methods employing cleavable electrophoretic tag reagents 审中-公开
    使用可切割电泳标签试剂的组合物和方法

    公开(公告)号：US20020142329A1

    公开(公告)日：2002-10-03

    申请号：US10008573

    申请日：2001-11-09

    Applicant: Aclara BioSciences, Inc.

    Inventor： Tracy Matray ,  Vincent Hernandez ,  Sharat Singh

    IPC: C12Q001/68 ,  G01N033/53 ,  C07H021/04 ,  C07K016/46

    CPC classification number: C07H21/04 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/68 ,  C40B20/08 ,  C40B40/08 ,  C40B50/16 ,  C40B70/00 ,  G01N33/561

    Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The methods employ compositions comprising luminescent molecules such as, for example, fluorescent molecules, which are modified to provide for electrophoretic properties that differ for each modified luminescent molecule while maintaining substantially the same absorption, emission and quantum yield properties of the original luminescent molecule. The compositions may be cleavably linked to binding molecules to form the e-tag probes.

    Abstract translation: 提供了用于多个检测一个或多个配体和靶抗配糖体的结合或相互作用的探针组。 检测涉及到识别标签作为目标识别的结果。 探针组包括电泳标签探针或e-标签探针，其包含检测区域和称为迁移率调节剂的迁移率限定区域，两者都连接到靶结合部分。 将目标抗配糖与一组电子标签探针接触，并用选择的切割剂处理接触的抗配糖剂，导致电子标签报告子与未切割和/或部分切割的e-标签探针的混合物。 将混合物暴露于有效结合未切割或部分切割的e-标签探针的捕获剂，然后进行电泳分离。 在多重测定中，可以分离和检测不同的释放的电子标签记录器，以提供目标识别。 该方法使用包含发光分子（例如荧光分子）的组合物，其被修饰以提供对于每个修饰的发光分子不同的电泳性质，同时保持原始发光分子的基本相同的吸收，发射和量子产率性质。 组合物可以与结合分子可裂解连接以形成e-标签探针。

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