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    Methods and apparatus for detecting polynucleotide hybridization
    1.
    发明申请
    Methods and apparatus for detecting polynucleotide hybridization 失效
    检测多核苷酸杂交的方法和装置

    公开(公告)号：US20010034025A1

    公开(公告)日：2001-10-25

    申请号：US09767316

    申请日：2001-01-22

    Applicant: LJL BioSystems, Inc.

    Inventor： Douglas N. Modlin ,  Todd E. French ,  Jon F. Petersen ,  John C. Owicki

    IPC: C12Q001/68 ,  G01N033/00

    CPC classification number: G01N35/028 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01L3/50853 ,  C12Q1/6816 ,  C12Q1/6827 ,  C40B60/14 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  Y10T436/143333 ,  C12Q2565/107 ,  C12Q2563/103 ,  C12Q2561/119

    Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) contacting a sample polynucleotide with a reference polynucleotide at an assay site, where at least one of the polynucleotides is capable of emitting luminescence, (2) illuminating the assay site with light capable of stimulating such luminescence, (3) detecting light transmitted from the assay site, and (4) deriving information relating to the extent of hybridization between the sample and reference polynucleotides based on the detected light. The methods may further include (1) illuminating with and/or detecting polarized light, (2) deriving information relating to the sequence of the sample polynucleotide from the extent of hybridization, and (3) converting the light to a signal and distinguishing between a portion of the signal attributable to luminescence and a portion attributable to background.

    Abstract translation: 用于在基于发光的测定中检测多核苷酸杂交的方法和装置。 方法可以包括（1）在测定位点使样品多核苷酸与参考多核苷酸接触，其中至少一个多核苷酸能够发射发光，（2）用能够刺激这种发光的光照射测定位点，（3 ）检测从测定位点传输的光，以及（4）基于检测到的光导出与样品和参考多核苷酸之间的杂交程度相关的信息。 所述方法还可以包括（1）用偏振光进行照射和/或检测偏振光，（2）从杂交程度导出与样品多核苷酸序列相关的信息，和（3）将光转换成信号并区分 可归因于发光的信号的一部分和归因于背景的部分。

    Apparatus and methods for spectroscopic measurements
    4.
    发明申请
    Apparatus and methods for spectroscopic measurements 失效
    用于光谱测量的装置和方法

    公开(公告)号：US20010007496A1

    公开(公告)日：2001-07-12

    申请号：US09765869

    申请日：2001-01-19

    Applicant: LJL BioSystems, Inc.

    Inventor： Douglas N. Modlin ,  Jon F. Petersen ,  John C. Owicki

    IPC: G01N021/64 ,  G01N021/25

    CPC classification number: B82Y15/00 ,  G01N21/253 ,  G01N21/55

    Abstract: Apparatus and methods for measuring light transmitted from a sample. The apparatus may include a stage, a light source, and a detector. The stage may be configured to hold a microplate having a plurality of sample wells. The apparatus may be configured to take measurements of one or more of absorbance, scattering, reflectance and luminescence. The apparatus may permit simultaneous measurements of two or more of these properties.

    Abstract translation: 用于测量从样品透射的光的装置和方法。 该装置可以包括舞台，光源和检测器。 该台可以被配置成容纳具有多个样品孔的微孔板。 该装置可以被配置为对吸光度，散射，反射率和发光中的一种或多种进行测量。 该装置可以允许同时测量两种或多种这些性质。

    Broad range light detection system
    7.
    发明申请
    Broad range light detection system 有权

    公开(公告)号：US20020056803A1

    公开(公告)日：2002-05-16

    申请号：US10004647

    申请日：2001-12-03

    Applicant: LJL BioSystems, Inc.

    Inventor： Douglas N. Modlin ,  David P. Stumbo ,  Rick V. Stellmacher

    IPC: G01J001/32

    CPC classification number: G01N21/6408 ,  G01N21/6445 ,  G01N21/6452

    Abstract: A broad-range light-detection system. In some embodiments, the system includes apparatus and methods for detecting light with high accuracy over a broad range of intensities. In other embodiments, the system includes apparatus and methods for automatically scaling the detection range to improve detection based on the intensity of the detected light. In yet other embodiments, the system includes apparatus and methods for detecting light with increased speed, particularly in applications involving analysis of successive samples.

    Apparatus and methods for identifying quenching effects in luminescence assays
    10.
    发明申请
    Apparatus and methods for identifying quenching effects in luminescence assays 审中-公开
    用于鉴定发光测定中猝灭效应的装置和方法

    公开(公告)号：US20010016330A1

    公开(公告)日：2001-08-23

    申请号：US09766131

    申请日：2001-01-19

    Applicant: LJL BioSystems, Inc.

    Inventor： John C. Owicki ,  Douglas N. Modlin ,  Todd E. French

    IPC: G01N033/53 ,  G01N033/542

    CPC classification number: G01N21/6408 ,  G01N21/76

    Abstract: Apparatus and methods for identifying and correcting for quenching in luminescence assays using luminescence lifetimes and/or luminescence intensities. One aspect of the invention involves identifying quenching using combinations of luminescence lifetimes and/or intensities. Another aspect of the invention involves correcting for quenching by eliminating false positives or false negatives due to quenching in luminescence assays.

    Abstract translation: 用于使用发光寿命和/或发光强度识别和校正在发光测定中淬灭的装置和方法。 本发明的一个方面涉及使用发光寿命和/或强度的组合识别猝灭。 本发明的另一方面涉及通过在发光测定中消除由于淬灭引起的假阳性或假阴性来校正猝灭。

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