우결핵 진단 키트 및 이를 이용한 우결핵 진단 방법
    4.
    发明公开
    우결핵 진단 키트 및 이를 이용한 우결핵 진단 방법 有权
    牛血管结核诊断试剂盒及其诊断方法

    公开(公告)号:KR1020120051196A

    公开(公告)日:2012-05-22

    申请号:KR1020100112506

    申请日:2010-11-12

    Abstract: PURPOSE: A kit for diagnosing bovine tuberculosis containing monoclonal antibodies and polyclonal antibodies is provided to effectively measure tuberculosis attack. CONSTITUTION: A monoclonal antibody which specifically binds to an epitope is denoted by an amino acid sequence of sequence number 8 in a bovine interferon gamma receptor-binding region. The monoclonal antibody is produced from hydridoma cells(deposit number KCTC 11784BP). A kit for diagnosing bovine contains monoclonal antibody or antigen binding fragment and anti-bovine interferon gamma polyclonal antibody.

    Abstract translation: 目的:提供用于诊断含有单克隆抗体和多克隆抗体的牛结核的试剂盒,以有效测量结核病的发作。 构成:特异性结合表位的单克隆抗体由牛干扰素γ受体结合区中的序列号8的氨基酸序列表示。 单克隆抗体由Hydridoma细胞(保藏号KCTC 11784BP)产生。 用于诊断牛的试剂盒含有单克隆抗体或抗原结合片段和抗牛干扰素γ多克隆抗体。

    돼지 유행성 설사 바이러스 항원 진단키트 및 진단 방법
    8.
    发明公开
    돼지 유행성 설사 바이러스 항원 진단키트 및 진단 방법 无效
    PORCINE流行病毒病毒诊断方法的测试工具

    公开(公告)号:KR1020080040942A

    公开(公告)日:2008-05-09

    申请号:KR1020060108942

    申请日:2006-11-06

    Abstract: A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).

    Abstract translation: 提供了一种用于诊断猪流行性腹泻病毒抗原的试剂盒和方法,用于在农场或实验室领域中没有特殊检测设备的情况下方便快速地诊断抗原。 一种用于诊断猪流行性腹泻病毒抗原的试剂盒的特征在于,将与流行性腹泻病毒和金颗粒的核衣壳58kDa偶联的单克隆抗体6G15(沉积编号KCTC 10996BP)的缀合物连接到猪的反应位置 将条带上的流行性腹泻病毒抗原和单克隆抗体9D11(沉积编号KCTC 10997BP)连接到与猪流行性腹泻病毒抗原和单克隆抗体6G15的偶联部分不同的位置。 用于诊断猪流行性腹泻病毒抗原的方法包括以下步骤:(a)将样品加载到条带上的检测垫上; (b)将与流行性腹泻病毒的金卡帕58kDa和金颗粒偶联的单克隆抗体6G15的缀合物与样品中的猪流行性腹泻病毒抗原反应; 和(c)使猪流行性腹泻病毒抗原,单克隆抗体9D11和从步骤(b)获得的反应产物反应。

    신속 면역크로마토그라피법에 의한 H5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법
    9.
    发明公开
    신속 면역크로마토그라피법에 의한 H5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법 有权
    用于H5型高致病性流感病毒性流行性感冒病毒和使用快速免疫印迹的一般流行性流感病毒抗原的诊断条带及其制备方法

    公开(公告)号:KR1020070072945A

    公开(公告)日:2007-07-10

    申请号:KR1020060000325

    申请日:2006-01-03

    Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.

    Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条,以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染,或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于,其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤:(a)分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; (b)将由步骤(a)获得的抗体吸收膜分别附着于金颗粒上,制备抗体 - 金缀合物,然后混合; 和(c)将由步骤(b)获得的缀合物沉淀在碱性材料中,然后干燥。

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