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公开(公告)号：JP2009102320A
公开(公告)日：2009-05-14
申请号：JP2008286959
申请日：2008-11-07
Inventor： SCHOLZ CHRISTIAN , ANDRES HERBERT , FAATZ ELKE , ENGEL ALFRED , SIZMANN DOROTHEA
IPC: C07K19/00 , C12N15/09 , A61K38/00 , A61K39/00 , A61K39/21 , A61K47/48 , A61P31/12 , A61P31/16 , A61P31/18 , A61P31/20 , A61P37/02 , C07K14/00 , C07K14/15 , C07K14/16 , C07K14/195 , C07K14/245 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/06 , C12N5/10 , C12N9/90 , C12N15/62 , C12P21/02 , G01N33/532 , G01N33/543 , G01N33/569
CPC classification number: G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
Abstract: PROBLEM TO BE SOLVED: To provide more retroviral surface glycoprotein epitopes, e.g. e-gp41 molecule or e-gp36 in a soluble type. SOLUTION: A method for producing the soluble complex containing an essentially insoluble target protein and a chaperone includes a step of mixing the essentially insoluble target protein and the chaperone selected from the group consisting of FkpA, an inducer and SlyD in a non-physiological buffer, and a step of solubilizing both of the protein and the chaperone and regulating the buffer to a physiological condition in which the formed protein-chaperone complex is soluble. COPYRIGHT: (C)2009,JPO&INPIT
Abstract translation: 要解决的问题：提供更多的逆转录病毒表面糖蛋白表位，例如 e-gp41分子或e-gp36为可溶型。 解决方案：一种生产含有基本上不溶性靶蛋白和伴侣的可溶性复合物的方法，包括将基本上不溶的靶蛋白和选自FkpA，诱导物和SlyD的伴侣混合在非天然存在的步骤中， 生理缓冲液，以及使蛋白质和伴侣两者溶解并将缓冲液调节成所形成的蛋白质 - 伴侣配基可溶解的生理条件的步骤。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2009100770A
公开(公告)日：2009-05-14
申请号：JP2009025509
申请日：2009-02-06
Inventor： SCHOLZ CHRISTIAN , ANDRES HERBERT , FAATZ ELKE , ENGEL ALFRED , SCHMITT URBAN , BAZARSUREN ARIUNA , SCHAARSCHMIDT PETER
IPC: C12N15/09 , C12P21/02 , A61K38/00 , A61K39/00 , A61K39/21 , A61K47/48 , A61P31/12 , A61P31/16 , A61P31/18 , A61P31/20 , A61P37/02 , C07K14/00 , C07K14/16 , C07K14/195 , C07K14/245 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/06 , C12N5/10 , C12N9/90 , C12N15/62 , G01N33/53 , G01N33/532 , G01N33/543 , G01N33/569
CPC classification number: G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
Abstract: PROBLEM TO BE SOLVED: To develop and provide an efficient alternative expression system usable in improved expression of a recombinant protein containing rsgp (retroviral surface glycoprotein) as a target protein and simultaneously suitable even for a target protein not so important. SOLUTION: A method for producing a fusion protein comprises the following steps: (a) a step of culturing a host cell having an expression vector containing an operably-linked recombinant DNA molecule, encoding a fusion protein and comprising at least one nucleotide sequence encoding a target polypeptide and at least one nucleotide sequence encoding an FKBP (FK-506 binding protein) chaperone on the upstream side thereof, (b) a step of inducing the expression of the fusion protein and thereby forming the fusion protein in the form of an inclusion body and (c) a step of purifying the fusion protein from the inclusion body. COPYRIGHT: (C)2009,JPO&INPIT
Abstract translation: 要解决的问题：开发并提供一种有效的替代表达系统，其可用于改进表达含有rsgp（逆转录病毒表面糖蛋白）作为靶蛋白的重组蛋白，并且甚至对于目标蛋白甚至也不重要。 解决方案：制备融合蛋白的方法包括以下步骤：（a）培养具有含有可操作地连接的重组DNA分子的表达载体的宿主细胞的步骤，编码融合蛋白并包含至少一个核苷酸 编码靶多肽的序列和编码上游侧的FKBP（FK-506结合蛋白）伴侣的至少一个核苷酸序列，（b）诱导融合蛋白的表达，从而形成形式的融合蛋白的步骤 的包涵体和（c）从包涵体纯化融合蛋白的步骤。 版权所有（C）2009，JPO＆INPIT
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公开(公告)号：JP2014211452A
公开(公告)日：2014-11-13
申请号：JP2014155200
申请日：2014-07-30
Applicant: エフ.ホフマン−ラ ロシュアーゲーF.Hoffmann−La Roche Aktiengesellschaft , F. Hoffmann-La Roche Ag , エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft
Inventor： KARL JOHANN , ANDRES HERBERT , URSULA GARCZAREK , WOLFGANG ROLLINGER , NORBERT WILD
IPC: G01N33/68
CPC classification number: G01N33/57419
Abstract: 【課題】インビトロで結腸直腸癌(CRC)の評価を補助し得る新規マーカーまたはマーカーパネルを提供すること。【解決手段】a)試料中のセプラーゼポリペプチドおよび/またはその断片の濃度および/または活性を測定する工程、b)該試料中の抗p53、オステオポンチンおよび/またはフェリチンの濃度および/または活性を測定する工程、c)工程(a)および(b)で測定された値を数学的に組み合わせる工程、およびd)CRCの検出において工程(a)および工程(b)の組み合わせた測定結果を使用する工程を含み、値を組み合わせるために使用される数学的方法が、判別分析(DA)(すなわち、線形-、二次-、正規化-DA)、カーネル法(すなわち、SVM)、ノンパラメトリック法(すなわち、k-最近傍分類法)、PLS(部分最小二乗)、ツリーベース法(すなわち、ロジック回帰、CART、ランダムフォレスト法、ブースト/バギング法)、一般化線形モデル(すなわち、ロジスティック回帰)、主成分ベース法(すなわち、SIMCA)、一般化加法モデル、ファジー論理ベース法、ニューラルネットワークおよび遺伝的アルゴリズムベース法から選択される、インビトロで結腸直腸癌(CRC)を検出するための方法。【選択図】なし
Abstract translation: 要解决的问题：提供允许体外辅助评估结肠直肠癌（CRC）的新标记或标记物组。解决方案：用于检测结肠直肠癌（CRC）的体外方法包括以下步骤：（a ）测量样品和/或其片段中的seprase多肽的浓度和/或其活性; （b）测量样品中的抗p53，其中的骨桥蛋白和/或铁蛋白和/或其活性的抗p53的浓度; （c）在数学上组合在步骤（a）和（b）中测量的值; 以及（d）使用在CRC检测中组合的具有步骤（a）和（b）的测量结果。 从判别分析（DA）（即线性，二次和标准化DA），内核方法（即SVM），非参数方法（ 即k最近邻算法），PLS（偏最小二乘法），树基方法（即逻辑回归，CART，随机森林法，升压/装袋法），泛化线性模型（即 ，逻辑回归），主成分基础法（即SIMCA），泛化加法模型，模糊逻辑基础法，神经网络和遗传算法基础法。
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公开(公告)号：JP2007322436A
公开(公告)日：2007-12-13
申请号：JP2007183508
申请日：2007-07-12
Inventor： SCHOLZ CHRISTIAN , ANDRES HERBERT , FAATZ ELKE , ENGEL ALFRED , SIZMANN DOROTHEA
IPC: C12N15/09 , G01N33/569 , A61K38/00 , A61K39/00 , A61K39/21 , A61K47/48 , A61P31/12 , A61P31/16 , A61P31/18 , A61P31/20 , A61P37/02 , C07K14/00 , C07K14/155 , C07K14/16 , C07K14/195 , C07K14/245 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/06 , C12N5/10 , C12N9/90 , C12N15/62 , C12P21/02 , G01N33/532 , G01N33/543
CPC classification number: G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
Abstract: PROBLEM TO BE SOLVED: To provide immunoassay using a variant of gp41 and a variant of gp36 (this is more easily treated, and/or especially is a soluble complex (comprises the variants and a chaperone selected from among the peptidyl-prolyl-isomerase class of chaperones)) under buffer conditions required for performing the immunoassay or for immunity. SOLUTION: The immunoassay is based on the double antigen bridge concept comprising a first antigen, containing a first chaperone-antigen complex and a second antigen, containing a second chaperone-antigen complex. COPYRIGHT: (C)2008,JPO&INPIT
Abstract translation: 要解决的问题：使用gp41的变体和gp36变体提供免疫测定（这更容易治疗，和/或特别是可溶性复合物（包括变体和选自肽基脯氨酰基的伴侣） - 异构酶类的伴侣））在进行免疫测定或免疫所需的缓冲条件下。 解决方案：免疫测定是基于双抗原桥概念，其包含含有第一伴侣 - 抗原复合物的第一抗原和含有第二伴侣 - 抗原复合物的第二抗原。 版权所有（C）2008，JPO＆INPIT
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公开(公告)号：JP2007125026A
公开(公告)日：2007-05-24
申请号：JP2006326846
申请日：2006-12-04
Inventor： SCHOLZ CHRISTIAN , ANDRES HERBERT , FAATZ ELKE , ENGEL ALFRED , SCHMITT URBAN , BAZARSUREN ARIUNA , SCHAARSCHMIDT PETER
IPC: C12N15/09 , C12P21/02 , A61K38/00 , A61K39/00 , A61K39/21 , A61K47/48 , A61P31/12 , A61P31/16 , A61P31/18 , A61P31/20 , A61P37/00 , A61P37/02 , A61P37/04 , A61P43/00 , C07K14/00 , C07K14/16 , C07K14/195 , C07K14/245 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/06 , C12N5/10 , C12N9/90 , C12N15/62 , G01N33/53 , G01N33/532 , G01N33/543 , G01N33/569
CPC classification number: G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
Abstract: PROBLEM TO BE SOLVED: To develop and provide an efficient alternative expression system usable in improved expression of a recombinant protein containing rsgp (retroviral surface glycoprotein) as a target protein and simultaneously suitable even for the target protein not so important. SOLUTION: A method for producing a fusion protein comprises the following steps: a. a step of culturing a host cell having an expression vector containing a recombinant DNA molecule encoding a fusion protein and comprising operably-linked at least one nucleotide sequence encoding a target polypeptide and at least one nucleotide sequence encoding an FKBP (FK-506 binding protein) chaperone on the upstream side thereof in which the FKBP chaperone is selected from the group consisting of FkpA, SlyD and a trigger factor and the fusion protein is deficient in a signal peptide sequence, b. a step of inducing the expression of the fusion protein and thereby forming the fusion protein in the form of an inclusion body and c. a step of purifying the fusion protein from the inclusion body. COPYRIGHT: (C)2007,JPO&INPIT
Abstract translation: 待解决的问题：开发和提供一种有效的替代表达系统，其可用于改进表达含有rsgp（逆转录病毒表面糖蛋白）作为靶蛋白的重组蛋白，并且甚至对靶蛋白也是如此重要。 解决方案：制备融合蛋白的方法包括以下步骤：a。 培养具有含有编码融合蛋白的重组DNA分子的表达载体的宿主细胞的步骤，并且包含可操作地连接的编码靶多肽的至少一个核苷酸序列和至少一个编码FKBP（FK-506结合蛋白）的核苷酸序列， 其中FKBP伴侣选自FkpA，SlyD和触发因子的上游侧的分子伴侣，融合蛋白在信号肽序列中不足，b。 诱导融合蛋白的表达，从而形成包涵体形式的融合蛋白的步骤，c。 从包含体纯化融合蛋白的步骤。 版权所有（C）2007，JPO＆INPIT
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Patent Agency Ranking
公开(公告)号：WO2010130839A1
公开(公告)日：2010-11-18
申请号：PCT/EP2010056730
申请日：2010-05-17
Applicant: HOFFMANN LA ROCHE , ROCHE DIAGNOSTICS GMBH , ANDRES HERBERT , KARL JOHANN , KLOECKNER JULIA , ROESSLER MARKUS , TACKE MICHAEL , THIEROLF MICHAEL
Inventor： ANDRES HERBERT , KARL JOHANN , KLOECKNER JULIA , ROESSLER MARKUS , TACKE MICHAEL , THIEROLF MICHAEL
IPC: G01N33/574 , G01N33/53
CPC classification number: G01N33/57423 , G01N2333/4727
Abstract: The present invention relates to the assessment of lung cancer. It discloses the use of protein CYBP in the assessment of lung cancer. It also relates to a method for assessing lung cancer in vitro using a liquid sample, derived from an individual by measuring CYBP in said sample. Measurement of CYBP can, e.g., be used in the early detection or in the follow-up of patients with lung cancer.
Abstract translation: 本发明涉及肺癌的评估。 它公开了蛋白CYBP在肺癌评估中的应用。 它还涉及一种通过在所述样品中测量CYBP在体外使用来自个体的液体样品来评估肺癌的方法。 CYBP的测量可以例如用于肺癌患者的早期检测或随访。
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公开(公告)号：WO0227317A3
公开(公告)日：2003-09-12
申请号：PCT/EP0111101
申请日：2001-09-26
Applicant: ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , ELTZ HERBERT VON DER
Inventor： HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC: A61K47/48 , C07K1/107 , G01N30/88 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/566 , G01N33/68
CPC classification number: C07K1/1077 , A61K47/643 , A61K47/6889 , G01N33/5306 , G01N33/531 , G01N33/68 , G01N33/6845 , Y10S435/961 , Y10S435/962
Abstract: The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomelecule-linker product of uniform stoichiometry in a pre-selected amount.
Abstract translation: 本发明涉及一种制备均匀化学计量学的生物分子 - 接头共轭物的方法。 其特别涉及由分子量在5kD至500kD之间的生物分子和亲水性接头分子所组成的共轭物，所述接头分子量为1至15kD，4至60个带电荷的残基，其特征在于所述共轭物包含 至少一种具有预选量的均匀化学计量的生物分子 - 接头产物。
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公开(公告)号：WO03000878A2
公开(公告)日：2003-01-03
申请号：PCT/EP0206957
申请日：2002-06-24
Applicant: ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE
Inventor： SCHOLZ CHRISTIAN , ANDRES HERBERT , FAATZ ELKE , ENGEL ALFRED , SCHMITT URBAN , BAZARSUREN ARIUNA , SCHAARSCHMIDT PETER
IPC: C12N15/09 , A61K38/00 , A61K39/00 , A61K39/21 , A61K47/48 , A61P31/12 , A61P31/16 , A61P31/18 , A61P31/20 , A61P37/02 , C07K14/00 , C07K14/16 , C07K14/195 , C07K14/245 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/06 , C12N5/10 , C12N9/90 , C12N15/62 , C12P21/02 , G01N33/532 , G01N33/543 , G01N33/569
CPC classification number: G01N33/56988 , A61K39/00 , A61K47/64 , C07K14/005 , C07K2319/00 , C12N9/90 , C12N2740/16122 , G01N33/54306 , G01N33/56911 , G01N33/56983 , G01N2333/005 , G01N2333/16 , G01N2333/195 , G01N2469/20
Abstract: The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor, methods of recombinant protein expression, the recombinant polypeptides thus obtained as well as to the use of such polypeptides.
Abstract translation: 本发明涉及在细菌如大肠杆菌中克隆和表达外源蛋白或多肽。 特别地，本发明涉及包含FKBP型肽基脯氨酰异构酶的表达工具，其选自FkpA，SlyD和触发因子，重组蛋白表达的方法，由此获得的重组多肽以及使用其 多肽。
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公开(公告)号：WO2009074276A2
公开(公告)日：2009-06-18
申请号：PCT/EP2008010386
申请日：2008-12-08
Applicant: ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , KARL JOHANN , ANDRES HERBERT , GARCZAREK URSULA , ROLLINGER WOLFGANG , WILD NORBERT
Inventor： KARL JOHANN , ANDRES HERBERT , GARCZAREK URSULA , ROLLINGER WOLFGANG , WILD NORBERT
IPC: G01N33/574
CPC classification number: G01N33/57419
Abstract: The present invention relates to a method for assessing colorectal cancer (CRC) in vitro comprising measuring in a sample the concentration and/or activity of a seprase polypeptide and/or fragments thereof and of either anti-p53 and/or osteopontin and/or Ferritin, of optionally one or more other marker of CRC, and using the combined measurement result in the assessment of CRC. Furthermore, it especially relates to a method for assessing CRC from a liquid sample, derived from an individual by measuring seprase and at least anti-p53, Ferritin and/or osteopontin in said sample. The method according to the present invention can, e.g., be used in the early detection of cancer by screening of asymptomatic individuals or in the surveillance of patients who undergo surgery.
Abstract translation: 本发明涉及体外评估结肠直肠癌（CRC）的方法，包括测量样品中seprase多肽和/或其片段以及抗p53和/或骨桥蛋白和/或铁蛋白的浓度和/或活性 ，任选地一种或多种CRC的其他标志物，并且将所述组合的测量结果用于评估CRC。 此外，本发明尤其涉及一种通过测量所述样品中的seprase和至少抗p53，铁蛋白和/或骨桥蛋白从来自个体的液体样品评估CRC的方法。 根据本发明的方法可以例如用于通过筛查无症状个体或监测接受手术的患者来早期检测癌症。
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公开(公告)号：WO2005124360A3
公开(公告)日：2006-05-26
申请号：PCT/EP2005006526
申请日：2005-06-17
Applicant: ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , PESTLIN GABRIELE , ANDRES HERBERT , BERNDT PETER , HAGMANN MARIE-LUISE , KARL JOHANN , LANGEN HANNO , THIEROLF MICHAEL , ZOLG WERNER
Inventor： PESTLIN GABRIELE , ANDRES HERBERT , BERNDT PETER , HAGMANN MARIE-LUISE , KARL JOHANN , LANGEN HANNO , THIEROLF MICHAEL , ZOLG WERNER
IPC: G01N33/574 , G01N33/68
CPC classification number: G01N33/57415 , G01N2333/99
Abstract: The present invention relates to the diagnosis of breast cancer. It discloses the use of protein TEBP (telomerase-binding protein p23) in the diagnosis of breast cancer. It relates to a method for diagnosis of breast cancer from a liquid sample, derived from an individual by measuring TEBP in said sample. Measurement of TEBP can, e.g., be used in the early detection or diagnosis of breast cancer.
Abstract translation: 本发明涉及乳腺癌的诊断。 它公开了蛋白TEBP（端粒酶结合蛋白p23）在乳腺癌诊断中的应用。 本发明涉及通过测定所述样品中的TEBP从个体得到的来自液体样品的乳腺癌诊断方法。 TEBP的测量可以例如用于乳腺癌的早期检测或诊断。
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