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公开(公告)号：JP2010193885A
公开(公告)日：2010-09-09
申请号：JP2010034501
申请日：2010-02-19
Inventor： FROEHLICH THOMAS , HEINDL DIETER , ROESLER ANGELIKA
CPC classification number: C12Q1/6844 , C12Q2523/319 , C12Q2525/197 , C12Q2563/149 , C12Q2565/537
Abstract: PROBLEM TO BE SOLVED: To provide an improved method and an improved reagent for simultaneously analyzing many nucleic acid molecules. SOLUTION: Beads each containing at least two sequence-specific amplification primers are provided; wherein at least one primer thereof has been bound to the beads by an inducibly cleavable linker via a functional moiety selected from the group consisting of carboxy-, aldehyde-, azide-, alkyne-, amino-, thiol-, maleimide-, sulfonylalkene-, iodoacetyl-, aminohydrazine-, hydroxyamino-, and maleimide-. COPYRIGHT: (C)2010,JPO&INPIT
Abstract translation: 要解决的问题：提供用于同时分析许多核酸分子的改进的方法和改进的试剂。 提供了各自含有至少两个序列特异性扩增引物的珠粒; 其中至少一个引物通过选自羧基，醛，叠氮化物，炔，氨基 - ，硫醇 - ，马来酰亚胺 - ，磺酰基烯 - ，碘乙酰基 - ，氨基肼 - ，羟基氨基 - 和马来酰亚胺 - 。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2006075167A
公开(公告)日：2006-03-23
申请号：JP2005261187
申请日：2005-09-08
Inventor： BOELL INGA , DEGEN ANJA , HEINDL DIETER , SAGNER GREGOR
CPC classification number: C12Q1/6818 , C12Q1/6851 , C12Q1/686 , C12Q2531/113 , C12Q2561/113 , C12Q2521/525 , C12Q2565/301
Abstract: PROBLEM TO BE SOLVED: To provide an improved performance in melting curve analysis in a quantitative real time PCR amplification and following later-stage amplification cycle. SOLUTION: The composition for amplifying and detecting a target nucleic acid comprises a thermostable DNA polymerase, a mixture of deoxynucleoside triphosphate, a buffer, at least two amplification primers and at least one first hybridization probe labeled with the first fluorescent substance, and further contains pyrophosphatase. COPYRIGHT: (C)2006,JPO&NCIPI
Abstract translation: 要解决的问题：在定量实时PCR扩增和后期扩增循环中提供改进的熔解曲线分析性能。 解决方案：用于扩增和检测靶核酸的组合物包含热稳定DNA聚合酶，脱氧核苷三磷酸盐，缓冲液，至少两个扩增引物和至少一个用第一荧光物质标记的第一杂交探针的混合物，以及 还含有焦磷酸酶。 版权所有（C）2006，JPO＆NCIPI
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公开(公告)号：JP2006055166A
公开(公告)日：2006-03-02
申请号：JP2005236516
申请日：2005-08-17
Inventor： BERGMANN FRANK , ESCHERICH ACHIM , HEINDL DIETER , KREBS JANE , DER ELTZ HERBERT VON
CPC classification number: C12Q1/689 , C12Q1/6834 , C12Q2537/159 , C12Q2565/101
Abstract: PROBLEM TO BE SOLVED: To provide a method and a compound for detection of biological molecules in a sample that decontaminates reagents, necessary for said detection, from biological molecules and/or maintains said reagents free of biological molecules. SOLUTION: The invention relates to the method for the detection of biological molecules in the sample that avoids the detection of biological molecules potentially present in the reagent necessary to detect the biological molecules in the sample, comprising steps of (a) providing a sample potentially comprising said biological molecule, (b) providing binding moieties coupled to the surface of a solid phase, (c) providing reagents necessary to detect the biological molecule, (d) adding said reagents to said sample, (e) detecting the biological molecules in the sample, wherein in step (c) or in step (d) or in step (c) and (d) said reagents are in physical contact with said binding moieties under conditions, whereby said biological molecules potentially present in said reagents bind to said binding moieties coupled to said surface of a solid phase. COPYRIGHT: (C)2006,JPO&NCIPI
Abstract translation: 要解决的问题：提供用于检测生物分子的方法和化合物，其用于从生物分子中除去所需检测所需的试剂和/或维持所述没有生物分子的试剂。 解决方案：本发明涉及检测样品中生物分子的方法，其避免检测可能存在于检测样品中的生物分子所必需的试剂中的生物分子，包括以下步骤：（a）提供 可能包含所述生物分子的样品，（b）提供与固相表面偶联的结合部分，（c）提供检测生物分子所需的试剂，（d）将所述试剂加入到所述样品中，（e） 分子，其中在步骤（c）或步骤（d）或步骤（c）和（d）中，所述试剂在条件下与所述结合部分物理接触，由此可能存在于所述试剂中的所述生物分子结合 到与所述固相表面偶联的所述结合部分。 版权所有（C）2006，JPO＆NCIPI
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公开(公告)号：JP2005058236A
公开(公告)日：2005-03-10
申请号：JP2004235542
申请日：2004-08-12
Inventor： ANKENBAUER WALTRAUD , MAIER THOMAS , DEUFEL ANNETTE , HEINDL DIETER , BETZL GISELA , SCHMUCK RAINER , SCHNEIDINGER BERND , STREY JESSICA
IPC: C12N15/09 , A61K38/45 , C07H21/04 , C07K14/195 , C07K19/00 , C11D3/386 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/22 , C12N15/00 , C12N15/11 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12P21/02 , C12Q1/68
CPC classification number: C12N9/1252
Abstract: PROBLEM TO BE SOLVED: To provide a peptide having an increased thermal stability and a thermally stable DNA polymerase activity and a method for forming a purified peptide. SOLUTION: Disclosed is a peptide having a specific amino acid sequence and a DNA polymerase activity. COPYRIGHT: (C)2005,JPO&NCIPI
Abstract translation: 待解决的问题：提供具有增加的热稳定性和热稳定的DNA聚合酶活性的肽以及形成纯化肽的方法。 解决方案：公开了具有特定氨基酸序列和DNA聚合酶活性的肽。 版权所有（C）2005，JPO＆NCIPI
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公开(公告)号：JP2011125337A
公开(公告)日：2011-06-30
申请号：JP2010280527
申请日：2010-12-16
Inventor： ALBERS ANDREAS , ANKENBAUER WALTRAUD , BERGMANN FRANK , FROEHNER STEFANIE , HEINDL DIETER
IPC: C12Q1/68 , C12N15/09 , C12N15/113
CPC classification number: C12Q1/686 , C12Q2525/207 , C12Q2525/186 , C12Q2525/155
Abstract: PROBLEM TO BE SOLVED: To provide an improved detection method for a low RNA molecule.
SOLUTION: The method for the detection of an RNA molecule comprises: (a) a step of providing a sample containing the RNA molecule; (b) a step of hybridizing a first polynucleotide to the RNA molecule; (c) a step of reversely transcribing the RNA molecule to generate a first strand cDNA; (d) a step of hybridizing a second polynucleotide to the first strand cDNA, wherein the second polynucleotide is 3'-inextensible oligonucleotide containing (i) 3'-moiety complementary to a part of the first strand cDNA and (ii) 5'-projection containing the sequence of a second primer binding site; (e) a step of obtaining an extension reaction product containing a sequence complementary to the second primer binding site from the first strand cDNA; (f) a step of amplifying the extension reaction product by using the second primer complementary to the first primer and second primer binding sites and by means of polymerase chain reaction; and (g) a step of detecting the amplification product by means of real-time fluorescence readout.
COPYRIGHT: (C)2011,JPO&INPITAbstract translation: 要解决的问题：为低RNA分子提供改进的检测方法。 解决方案：用于检测RNA分子的方法包括：（a）提供含有RNA分子的样品的步骤; （b）将第一多核苷酸与RNA分子杂交的步骤; （c）逆转录RNA分子以产生第一链cDNA的步骤; （d）将第二多核苷酸与第一链cDNA杂交的步骤，其中第二个多核苷酸是3'不可扩展的寡核苷酸，其含有（i）与第一链cDNA的一部分互补的3'-部分，和（ii） 包含第二引物结合位点序列的突出物; （e）从第一链cDNA获得含有与第二引物结合位点互补的序列的延伸反应产物的步骤; （f）通过使用与第一引物和第二引物结合位点互补的第二引物并通过聚合酶链反应来扩增延伸反应产物的步骤; 和（g）通过实时荧光读数检测扩增产物的步骤。 版权所有（C）2011，JPO＆INPIT
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Patent Agency Ranking
公开(公告)号：JP2010252790A
公开(公告)日：2010-11-11
申请号：JP2010089441
申请日：2010-04-08
Inventor： ANKENBAUER WALTRAUD , HEINDL DIETER , JOSEL HANS-PETER DR , WEILKE CHRISTIAN
IPC: C12Q1/68 , C07D498/14 , C09B19/00 , C09B67/20 , C09B67/22 , C09K11/06 , G01N21/64 , G01N33/542
CPC classification number: C12Q1/6818 , C12Q1/6848 , C12Q2565/101 , C12Q2563/131 , C12Q2545/101 , C12Q2537/157 , C12Q2565/501
Abstract: PROBLEM TO BE SOLVED: To provide kits and methods for composition ratio control based on dyes that are designed to enable energy transfer between each other. SOLUTION: There are provided kits for composition ratio control including (a) a first dye having a first affinity group, wherein the first dye is excitable by first excitation light to emit radiation or to transfer energy to a second dye, (b) a second dye having a second affinity group, wherein the second dye is excitable by a second excitation light or by energy transfer from the first dye. The kits are characterized in that the first dye and the second dye are configured such that the first and the second affinity group have affinity to bind to each other, wherein energy transfer between the dyes is enabled upon binding of the first affinity group to the second affinity group and a mixture of the first dye and the second dye emits radiation upon excitation with the first or the second excitation light, wherein the radiation is a measure for the composition ratio of the first and the second dye. COPYRIGHT: (C)2011,JPO&INPIT
Abstract translation: 要解决的问题：提供基于设计成能够彼此能量传递的染料的组成比控制的试剂盒和方法。 提供了用于组成比例控制的试剂盒，其包括（a）具有第一亲和基团的第一染料，其中第一染料可通过第一激发光激发以发射辐射或将能量转移到第二染料，（b ）具有第二亲和基团的第二染料，其中所述第二染料可通过第二激发光或通过来自所述第一染料的能量转移而激发。 试剂盒的特征在于，第一染料和第二染料被配置为使得第一和第二亲和基团具有彼此结合的亲和力，其中在第一亲和基团与第二亲和基团结合时能够在染料之间传递能量 亲和基团和第一染料和第二染料的混合物在用第一或第二激发光激发时发射辐射，其中辐射是第一和第二染料的组成比的量度。 版权所有（C）2011，JPO＆INPIT
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公开(公告)号：JP2009225796A
公开(公告)日：2009-10-08
申请号：JP2009065018
申请日：2009-03-17
Inventor： ANKENBAUER WALTRAUD , HEINDL DIETER , LAUE FRANK
CPC classification number: C12P19/34 , C12Q1/6848 , C12Q1/686 , C12Q2525/179 , C12Q2525/101
Abstract: PROBLEM TO BE SOLVED: To provide an improved substitutive composition for a hot start PCR enabling inhibition of unspecified priming and primer extension not only before amplification process itself but in heat cycling process, and to provide a method for the same.
SOLUTION: Provided is the composition which includes a DNA polymerase, deoxynucleotides, at least one primer oligonucleotide, and randomized 5-8 mer oligonucleotide characterized in that the oligonucleotide includes a modification with an organic hydrophobic moiety.
COPYRIGHT: (C)2010,JPO&INPITAbstract translation: 要解决的问题：提供改进的用于热启动PCR的替代组合物，其不仅在扩增过程本身之前但是在热循环过程中能够抑制未指定的引发和引物延伸，并提供其用于其的方法。 解决方案：提供包含DNA聚合酶，脱氧核苷酸，至少一种引物寡核苷酸和随机化5-8mer寡核苷酸的组合物，其特征在于所述寡核苷酸包括具有有机疏水部分的修饰。 版权所有（C）2010，JPO＆INPIT
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公开(公告)号：JP2005200405A
公开(公告)日：2005-07-28
申请号：JP2004348896
申请日：2004-12-01
Inventor： HEINDL DIETER , REISER ASTRID , TABITI KARIM , JUNIUS MARTINA , HAUBER ALEXANDER
CPC classification number: C07H21/00 , Y10S436/808
Abstract: PROBLEM TO BE SOLVED: To provide an oligonucleotide having an improved melting curve behavior, especially a FRET hybridization probe.
SOLUTION: This method for synthesizing the oligonucleotide containing an axial molecular rod which has a rigid structure and can not bend by itself, comprises a process of providing a controlled hole glass particles and a process of synthesizing by a phosphoramidite chemical action. This oligonucleotide containing the axial molecular rod which has the rigid structure and can not bend by itself, in which the rod is covalently bonded at the 5' position of 5' terminal residue or at the 3' position of 3' terminal through a phosphate part, can be used as a primer for a primer elongation reaction or nucleic acid amplification reaction, and can also be used as a hybridization probe by which the temperature dependence of the hybridization can be monitored in real time.
COPYRIGHT: (C)2005,JPO&NCIPIAbstract translation: 要解决的问题：提供具有改善的熔解曲线行为的寡核苷酸，特别是FRET杂交探针。 解决方案：用于合成含有具有刚性结构并且不能自身弯曲的轴向分子棒的寡核苷酸的方法包括提供受控空穴玻璃颗粒的方法和通过亚磷酰胺化学作用合成的方法。 该寡核苷酸含有具有刚性结构并且不能自身弯曲的轴向分子棒，其中棒在5'末端残基的5'位置或3'末端的3'位置通过磷酸酯部分共价键合 可用作引物延伸反应或核酸扩增反应的引物，也可用作可以实时监测杂交温度依赖性的杂交探针。 版权所有（C）2005，JPO＆NCIPI
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公开(公告)号：JP2004065262A
公开(公告)日：2004-03-04
申请号：JP2003286973
申请日：2003-08-05
Inventor： HEINDL DIETER , JOSEL HANS-PETER DR , SAGNER GREGOR , BECHLER INGRID
IPC: G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/542 , G01N33/566 , G01N33/58
CPC classification number: C12Q1/6818
Abstract: PROBLEM TO BE SOLVED: To provide an improved FRET hybridization probe designed so that an absolute signal is not affected by the base composition and sequence of a target nucleic acid. SOLUTION: A FRET hybridization probe pair is a FRET hybridization probe pair comprising a substantially complementary nucleotide sequence substance to the target nucleic acid sequence, a fluorescence substance being either one of a FRET donor substance or a FRET acceptor substance, and a spacer substance connecting the nucleotide substance with the fluorescence substance respectively. The FRET hybridization probe pair is adjacently hybridizable to the target nucleic acid sequence. The spacer substances between the two members of the FRET hybridization probe pair can mutually form non-covalent bonding interaction. COPYRIGHT: (C)2004,JPO
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公开(公告)号：JP2009232871A
公开(公告)日：2009-10-15
申请号：JP2009170421
申请日：2009-07-21
Inventor： SAGNER GREGOR , BECHLER INGRID , BOLTE JOACHIM , HEINDL DIETER , JOSEL HANS-PETER , GUTEKUNST MARTIN , SEIBL RUDOLF , MUELLER CHRISTOPH
CPC classification number: C12Q1/6851 , G01N2021/6421 , C12Q2565/101 , C12Q2561/113 , C12Q2537/143
Abstract: PROBLEM TO BE SOLVED: To provide an improved system for optimizing multiplex/multi-collar detecting experiments, and at the same time, allowing a flexible design.
SOLUTION: The real time PCR device comprises at least one light source radiating the light toward a reaction container containing a fluorescent compound, an excitation unit placed to receive the light from the reaction container and containing an optical pipe uniformly distributing the light for delivering the light to a bundle of optical fibers, a detecting unit containing at least five independent fluorescence detecting entities, the detecting entities each having central detecting wavelengths differing from each other at least by 25 nm, a plurality, at least five, of optical fiber bundles arranged to receive the uniformly distributed light from the optical pipe and to transmit the light to the fluorescence detecting entities, a means for heating and cooling, and a plurality of reaction containers for containing reaction mixtures, wherein the plurality of the detecting entities can simultaneously detect a maximum fluorescence of at least five different fluorescent compounds, and the excitation unit and the detecting unit locate in separated housings.
COPYRIGHT: (C)2010,JPO&INPITAbstract translation: 要解决的问题：提供一种改进的系统，用于优化多重/多领检测实验，同时允许灵活的设计。 解决方案：实时PCR装置包括至少一个光源，将光朝向包含荧光化合物的反应容器辐射，激励单元被放置成接收来自反应容器的光，并且包含均匀分布光的光管 将光传送到一束光纤，检测单元包含至少五个独立的荧光检测实体，所述检测实体各自具有至少25nm彼此不同的中心检测波长，多个至少五个光纤 束，其布置成接收来自光管的均匀分布的光并将光透射到荧光检测实体，用于加热和冷却的装置以及用于容纳反应混合物的多个反应容器，其中所述多个检测实体可以同时 检测至少五种不同荧光化合物的最大荧光，和t 他的激励单元和检测单元位于分离的外壳中。 版权所有（C）2010，JPO＆INPIT
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