公开(公告)号：WO2017001536A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065232

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： PEUMANS, Peter ,  LAGAE, Liesbet ,  VAN ROY, Willem ,  STAKENBORG, Tim ,  VAN DORPE, Pol

IPC: G01N21/17 ,  G01N21/64

CPC classification number: G01N21/1717 ,  G01N21/645 ,  G01N21/648 ,  G01N2021/1731

Abstract: A sensor device (100) for quantifying luminescent targets comprises a light source (111), a detector (120), a modulator (130), and a processor (140). The light source (111) is adapted for exciting the luminescent target. The detector (120) is adapted for detecting the luminescence of the luminescent target resulting in a measured signal which comprises a desired signal originating from the luminescent target and a background signal. The modulator (130) is adapted for modulating a physical parameter resulting in a modulation of the desired signal which is different from the modulation of the background signal. The processor (140) is configured to correlate the modulation of the physical parameter with the modulation of the desired signal and/or the modulation of the background signal.

Abstract translation: 用于量化发光靶的传感器装置（100）包括光源（111），检测器（120），调制器​​（130）和处理器（140）。 光源（111）适于激发发光靶。 检测器（120）适于检测发光目标的发光，从而产生包含源自发光目标的期望信号和背景信号的测量信号。 调制器（130）适于调制产生与背景信号的调制不同的期望信号的调制的物理参数。 处理器（140）被配置为将物理参数的调制与期望信号的调制和/或背景信号的调制相关联。

公开(公告)号：WO2017029789A1

公开(公告)日：2017-02-23

申请号：PCT/JP2016/003655

申请日：2016-08-08

Applicant: PANASONIC CORPORATION ,  IMEC VZW

Inventor： SATO, Shuji ,  OKUMURA, Yasuaki ,  NISHIYAMA, Yukari ,  KAWAMURA, Tatsurou ,  JONES, Ben ,  LAGAE, Liesbet ,  STAKENBORG, Tim

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/041 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/0861 ,  B01L2400/0406 ,  B01L2400/0688 ,  G01N21/03 ,  G01N27/416 ,  G01N33/54386

Abstract: The present invention provides a novel micro analysis chip and a fabrication method thereof. The micro analysis chip comprises an inlet and a fluid flow path which communicates thereto. The fluid flow path comprises a first flow path, a second flow path, and a third flow path which are arranged continuously along a longitudinal direction of the fluid flow path. An antibody is bound on at least one peripheral surface selected from the group consisting of a peripheral surface of the second flow path and a peripheral surface of the third flow path. A cross-sectional area of the third flow path is constant or increased monotonically along a direction X from the second flow path toward the third flow path. A cross-sectional area of the second flow path is increased monotonically along the direction X from the one end to the other end of the second flow path. A cross-sectional area of the first flow path is larger than a cross-sectional area at the one end of the second flow path.

Abstract translation: 本发明提供了一种新颖的微分析芯片及其制造方法。 微分析芯片包括与其通信的入口和流体流动路径。 流体流动路径包括沿着流体流动路径的纵向方向连续布置的第一流动路径，第二流动路径和第三流动路径。 抗体结合在选自第二流路的外周表面和第三流路的外周表面的至少一个外周表面上。 第三流路的横截面积沿着从第二流路朝向第三流路的方向X单调地恒定或增加。 第二流路的截面积从第二流路的一端向另一端沿方向X单调增加。 第一流路的截面积大于第二流路一端的截面积。

公开(公告)号：WO2017001534A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065229

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： PEUMANS, Peter ,  LAGAE, Liesbet ,  VAN ROY, Willem ,  STAKENBORG, Tim ,  VAN DORPE, Pol

IPC: G01N21/64 ,  G01N21/27

CPC classification number: G01N21/6408 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/648 ,  G01N2021/6413 ,  G01N2021/6439

Abstract: A sensor device (100) for quantifying luminescent targets. The device comprises a light source (111) for exciting the targets, thus generating luminescence signals and a detector (120) for detecting these signals, resulting in a detected signal which comprises a desired signal (310) originating from the targets and a background signal (320). It moreover comprises a bleaching device (135) for bleaching of at least part of the sources generating the background signal (320) and a processor (140) configured to trigger the bleaching device (135) to start bleaching, and to trigger the light source (111) for exciting the remaining luminescent targets which are not bleached, and to trigger the detector (120) for detecting the luminescence signal of the remaining luminescent targets, so as to generate a measurement signal representative for the quantification of the luminescent targets.

Abstract translation: 一种用于量化发光靶的传感器装置（100）。 该装置包括用于激发目标的光源（111），从而产生发光信号，以及用于检测这些信号的检测器（120），产生检测信号，该检测信号包括源自目标的期望信号（310）和背景信号 （320）。 它还包括漂白装置（135），用于漂白产生背景信号的源的至少一部分（320）和配置成触发漂白装置（135）开始漂白的处理器（140），并触发光源 （111），用于激发未漂白的剩余发光靶，并且触发检测器（120）以检测剩余的发光靶的发光信号，以产生代表发光靶的量化的测量信号。

公开(公告)号：WO2016203051A1

公开(公告)日：2016-12-22

申请号：PCT/EP2016/064217

申请日：2016-06-20

Applicant: IMEC VZW

Inventor： JONES, Benjamin ,  STAKENBORG, Tim ,  FIORINI, Paolo

IPC: B01L3/00 ,  F16K99/00

CPC classification number: B01L3/502707 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00414 ,  B01J2219/00418 ,  B01J2219/00637 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/16 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  B05C7/04 ,  F16K99/0021

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Abstract translation: 描述了用于局部涂覆流体通道的内表面的流体装置（100）。 流体装置（100）包括在公共接头（105）处相交的第一（101），第二（102）和第三（103）流体通道。 第一流体通道可连接到涂层流体储存器，并且第三流体通道可连接到样品流体储存器。 流体装置（100）还包括流体控制装置（111），其配置用于在公共接头（105）处产生用于涂覆流体的流体流路，使得当涂覆时，涂覆流体从第一（101）传播到 所述第二（102）流体通道经由所述公共接头（105）而不传播到所述第三（103）流体通道中。 已经公开了用于涂覆和感测的相应方法。

公开(公告)号：WO2022241138A1

公开(公告)日：2022-11-17

申请号：PCT/US2022/029026

申请日：2022-05-12

Applicant: JANSSEN BIOTECH, INC. ,  IMEC VZW

Inventor： PEUMANS, Peter ,  TULLOCH, Alexander ,  HENRY, Olivier ,  VAN ROY, Willem ,  STAKENBORG, Tim

IPC: C12M1/00 ,  B01L3/00 ,  G01N15/14 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/086 ,  B01L2400/088 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M35/04 ,  C12M47/04 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2015/149 ,  G03H1/00

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological ceils. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

公开(公告)号：WO2018115040A1

公开(公告)日：2018-06-28

申请号：PCT/EP2017/083681

申请日：2017-12-20

Applicant: IMEC VZW

Inventor： STAKENBORG, Tim ,  FIORINI, Paolo

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/686

Abstract: A method for analyzing a fluid sample, the method comprising obtaining (710) the fluid sample in at least one cavity of a substrate and introducing also buffers and/or reagents in said cavity, performing (720) nucleic acid extraction and/or purification in said cavity, and performing (730) nucleic acid amplification in the same cavity. A corresponding system also is claimed.

公开(公告)号：WO2017001642A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065449

申请日：2016-06-30

Applicant: IMEC VZW

Inventor： VAN ROY, Willem ,  STAKENBORG, Tim ,  COVENS, Kris

IPC: G01N33/543

CPC classification number: G01N33/54393 ,  C12Q1/002 ,  C12Q1/6825 ,  G01N33/523

Abstract: A device (1) for sensing an analyte, the device (1) comprises at least a sample inlet (10) for receiving a sample, affinity probes (111) selected to have a preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte, the transducer not being a FET transducer, and a desalting unit (13) for desalting the received sample.

Abstract translation: 一种用于感测分析物的装置（1），所述装置（1）至少包括用于接收样品的样品入口（10），被选择为具有优先结合分析物的亲和探针（111），敏感器（11） 涉及分析物和/或附着于分析物的标签的特性，换能器不是FET换能器，以及用于对接收到的样品进行脱盐的脱盐单元（13）。

公开(公告)号：WO2019180202A1

公开(公告)日：2019-09-26

申请号：PCT/EP2019/057240

申请日：2019-03-22

Applicant: IMEC VZW

Inventor： STAKENBORG, Tim ,  CHEN, Chang ,  COVENS, Kris ,  CAI, Qing ,  FAUVART, Maarten

IPC: B01L7/00 ,  C12Q1/6869

Abstract: The present invention relates to a method for writing data comprising a sequence of bits, the data being written in a form of nucleic acid, by in-vitro enzymatically producing memory nucleic acid from a strand of memory writing substrate nucleic acid, wherein the strand of memory writing substrate nucleic acid comprises a plurality of spacer sections and memory writing sections sandwiched between the spacer sections. Each of the spacer sections comprises one or more nucleobases, and each of the memory writing sections comprises a nucleobase other than the nucleobases of an adjacent spacer section upstream of the memory writing section in a travel direction of an enzyme along the strand of memory writing substrate nucleic acid. The method comprising: repeating of: synthesising, in liquid medium comprising the strand of memory writing substrate nucleic acid contacted with the enzyme, a spacer portion of the memory nucleic acid from a spacer section by the enzyme by contacting with a solution of spacer nucleotides compatible with the nucleobases of the spacer section; halting the synthesising of the spacer portion in a position where the enzyme is reaching the memory writing section resulting from incompatibility between spacer nucleotides and nucleobases of the portion of the memory nucleic acid from the memory writing section; receiving a sub-sequence of the sequence of bits, said sub-sequence comprising at least one bit; selecting a memory nucleotide compatible with the nucleobase of the memory writing section, and comprising a first label or first modification, on a condition that said sub-sequence comprises a predetermined first sequence of bit-values, and selecting a memory nucleotide compatible with the nucleobase of the memory writing section, and comprising a second label or second modification, on a condition that said sub-sequence comprises a predetermined second sequence of bit-values; and subsequent to the halting, synthesising, in the liquid medium comprising the strand of memory writing substrate nucleic acid contacted with the enzyme, a memory portion of the memory nucleic acid from the memory writing section by the enzyme by contacting the enzyme with a solution of the selected memory nucleotide.

公开(公告)号：WO2017001526A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/065219

申请日：2016-06-29

Applicant: IMEC VZW

Inventor： PEUMANS, Peter ,  LAGAE, Liesbet ,  VAN ROY, Willem ,  STAKENBORG, Tim ,  VAN DORPE, Pol

IPC: G01N21/64 ,  G01N21/17

CPC classification number: G01N21/6452 ,  G01N21/1717 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/648 ,  G01N2021/1731 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439

Abstract: A sensor device (100) for quantifying luminescent targets configured in an at least one dimensional pattern. The sensor device comprises a detector (120) for obtaining an at least one dimensional pattern of measured signals, wherein the detector (120) is adapted for detecting the luminescence of the luminescent targets, resulting in a measured pattern. The sensor device moreover comprises a processor (140) configured to correlate the measured pattern with at least one reference pattern, so as to generate a measurement signal representative for the quantification of luminescent targets. The at least one reference pattern is a recorded pattern or an expected pattern. A recorded pattern is a pattern which is obtained by the detector before the measured pattern is obtained.

Abstract translation: 一种用于量化以至少一维图案配置的发光目标的传感器装置（100）。 传感器装置包括用于获得测量信号的至少一维图案的检测器（120），其中检测器（120）适于检测发光靶的发光，产生测量图案。 传感器装置还包括被配置成将所测量的图案与至少一个参考图案相关联的处理器（140），以产生代表发光目标的量化的测量信号。 至少一个参考图案是记录图案或预期图案。 记录图案是在获得测量图案之前由检测器获得的图案。

公开(公告)号：WO2017001374A1

公开(公告)日：2017-01-05

申请号：PCT/EP2016/064953

申请日：2016-06-28

Applicant: IMEC VZW

Inventor： VOS, Rita ,  JANS, Karolien ,  STAKENBORG, Tim

IPC: G01N33/543 ,  G01N33/547

CPC classification number: G01N33/54353 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00677 ,  B01J2219/00725 ,  B01J2219/0074 ,  G01N33/531 ,  G01N33/54386

Abstract: A method for immobilizing an analyte-recognizing molecule (1) on a surface (2') functionalized with chemical groups Y 1 suitable for reacting with a chemical group X 2 of a coupling molecule (7) to form a reaction product comprising a chemical group Y 2 suitable for reacting with the analyte-recognizing molecule (1), the method comprising the steps of: a) Providing the functionalized surface (2'), b) Contacting the functionalized surface (2') with a solution (6) comprising simultaneously: i) The coupling molecule (7), and ii) The analyte-recognizing molecule (1).

Abstract translation: 一种用于将分析物识别分子（1）固定在用适于与偶联分子（7）的化学基团X2反应的化学基团Y1官能化的表面（2'）上的方法，以形成包含化学基团Y2的反应产物 与所述分析物识别分子（1）反应，所述方法包括以下步骤：a）提供所述官能化表面（2'），b）使所述官能化表面（2'）与溶液（6）接触，所述溶液（6）同时包含：i ）偶联分子（7），和ii）分析物识别分子（1）。