公开(公告)号：KR20120025257A

公开(公告)日：2012-03-15

申请号：KR20100087544

申请日：2010-09-07

Applicant: MICO BIO MED CO LTD ,  KOREA RES INST OF BIOSCIENCE ,  KOMICO LTD

Inventor： RHA KWAN GOO ,  KIM YONG KYU ,  JEON BU IL ,  PARK HYUN KYU ,  CHUNG BONG HYUN ,  SHIN YONG BEOM

IPC: G01N21/27 ,  G02B5/09

CPC classification number: G01N21/553 ,  G01N21/27 ,  G01N2021/258 ,  G02B5/09

Abstract: PURPOSE: A surface plasmon resonance sensor system is provided to attach a sample feeding part to a sensor chip because a control part for controlling distance is installed in the sample feeding part. CONSTITUTION: A surface plasmon resonance sensor system comprises a light source(100), a polarizing part(110), a rotary mirror(200), a shielding layer(300), a cylinder lens(400), a sensor chip(500), and a light receiving part(600). The light source generates a first light(120). The polarizing part polarizes the first light. The rotary mirror comprises a substrate and metal layers deposited on at least one surface. The shielding layer is arranged adjacently to the rotary mirror and a part of a second light(240) passes through the shielding layer. The cylinder lens collects the part of the second light passing through the shielding layer. The sensor chip a prism(510) and a metallic thin film(530) forming in one side of the prism. The light receiving part detects a third light(610).

Abstract translation: 目的：提供表面等离子体共振传感器系统，用于将样品供给部分附接到传感器芯片，因为用于控制距离的控制部件安装在样品供给部分中。 构成：表面等离子体共振传感器系统包括光源（100），偏振部分（110），旋转镜（200），屏蔽层（300），柱面透镜（400），传感器芯片（500） ，以及光接收部（600）。 光源产生第一光（120）。 极化部分使第一光偏振。 旋转镜包括沉积在至少一个表面上的基底和金属层。 屏蔽层被布置成与旋转镜相邻，并且第二光（240）的一部分穿过屏蔽层。 柱面透镜聚集通过屏蔽层的第二光的一部分。 传感器芯片在棱镜的一侧形成棱镜（510）和金属薄膜（530）。 光接收部检测第三光（610）。

公开(公告)号：WO2010008238A2

公开(公告)日：2010-01-21

申请号：PCT/KR2009003960

申请日：2009-07-17

Applicant: KOREA RES INST OF BIOSCIENCE ,  INFOPIA CO LTD ,  SHIN YONG BEOM ,  KIM MIN GON ,  BAE BYEONG WOO

Inventor： SHIN YONG BEOM ,  KIM MIN GON ,  BAE BYEONG WOO

IPC: G01N30/95

CPC classification number: G01N33/54373 ,  G01N21/7703 ,  G01N30/95 ,  G01N33/558 ,  G01N2021/7766

Abstract: The present invention relates to an immunochromatographic detection sensor comprising optical waveguides and a detection method using the same, and more particularly, to an immunochromatographic detection sensor comprising optical waveguides, in which the optical waveguides are provided under the membrane, probe beams transmitted through the optical waveguide maximize the interaction frequency between evanescent wave generated on the surface of optical waveguide and the colored conjugate in the band formed on the membrane, and thus the absorbance signal from the colored conjugate is greatly amplified to improve the sample detection sensitivity, and a detection method using the same.

Abstract translation: 本发明涉及包括光波导的免疫层析检测传感器和使用其的检测方法，并且更具体地涉及包括光波导的免疫层析检测传感器，其中光波导设置在膜下方，探测光束透过光学 波导使在光波导表面上产生的eva逝波和在膜上形成的带中的彩色共轭物之间的相互作用频率最大化，并且因此来自彩色共轭物的吸收信号被极大地放大以提高样品检测灵敏度，以及检测方法 使用相同的。

公开(公告)号：WO2011149249A3

公开(公告)日：2012-04-19

申请号：PCT/KR2011003799

申请日：2011-05-24

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  SHIN YONG BEOM ,  AHN JUN HYOUNG ,  LEE TAE HAN

Inventor： KIM MIN GON ,  SHIN YONG BEOM ,  AHN JUN HYOUNG ,  LEE TAE HAN

IPC: G01N27/327 ,  C12Q1/68 ,  G01N27/406 ,  G01N33/48

CPC classification number: G01N27/127 ,  G01N33/54306 ,  G01N33/5438

Abstract: The present invention relates to an electric biosensor for detecting an infinitesimal sample. More specifically, the present invention relates to a method for electrical detection of an infinitesimal sample, comprising the steps of: (a) preparing a biosensor by fixing, to an electrically insulated nano-electrode chip, a receptor molecule that is selectively coupled to a target substance to be subjected to an assay; (b) adding a sample that contains the target substance selectively coupled with the receptor molecule to the biosensor for reaction; (c) subjecting the nano-electrode chip that has been reacted with the target substance-containing sample to treatment and reaction with gold nanoparticles; (d) treating peripheral regions of the reacted gold nanoparticles with gold ions to induce reduction of the gold ions; and (e) measuring the electric conductivity or impedance of the nano-electrode chip where the gold ions have been reduced, thereby detecting a target substance that is specifically bonded to the receptor molecule. The method for electrical detection of an infinitesimal sample using the biosensor according to the present invention not only interconnects nano-electrodes through a single reduction reaction between gold nanoparticles and gold ions, but it also electrically interconnects the electrically insulated nano-electrodes such that the electrical conductivity is increased. As a result, the measurement sensitivity is high, the quantitative assay of an infinitesimal sample becomes possible, and a selective antigen-antibody bond can be measured with a very simple, electrical method.

Abstract translation: 本发明涉及用于检测无限小样本的电生物传感器。 更具体地说，本发明涉及无限小样品的电检测方法，包括以下步骤：（a）通过将绝缘的纳米电极芯片固定到选择性地耦合到 目标物质进行测定; （b）将含有与受体分子选择性偶联的目标物质的样品加入生物传感器进行反应; （c）使已经与含靶物质的样品反应的纳米电极芯片与金纳米颗粒进行处理和反应; （d）用金离子处理反应的金纳米颗粒的外围区域以诱导金离子的还原; 和（e）测量金离子已经还原的纳米电极芯片的电导率或阻抗，从而检测特异性结合于受体分子的目标物质。 根据本发明的使用生物传感器的无限小样品的电检测方法不仅通过金纳米颗粒和金离子之间的单一还原反应来互连纳米电极，而且还使电绝缘的纳米电极电互连，使得电气 电导率增加。 结果，测量灵敏度高，无限小样品的定量测定成为可能，并且可以用非常简单的电气方法测量选择性抗原 - 抗体结合。

公开(公告)号：WO2012023820A3

公开(公告)日：2012-05-18

申请号：PCT/KR2011006099

申请日：2011-08-18

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  LI TAIHUA ,  SHIN YONG BEOM

Inventor： KIM MIN GON ,  LI TAIHUA ,  SHIN YONG BEOM

IPC: C12N9/00 ,  B01J37/34

CPC classification number: C12N11/08 ,  C12N13/00

Abstract: The present invention relates to a method for activating a catalyst using the photothermal properties of photothermal nanomaterials, wherein light is irradiated to a catalyst-photothermal nanomaterials complex to activate a catalyst at a temperature at which the catalyst has low activity or no activity. According to the present invention, the method for activating a catalyst enables the activation of a catalyst by increasing the temperature of only the surroundings of nanomaterials while the temperature of the entire reaction media is changed slightly. According to the method, it is possible to carry out a catalytic reaction, which normally shows high activity at room temperature, with high activity even at low temperatures. That is, the present invention can provide a method for enabling a catalyst to have high activity even at low temperature and extremely cold areas by fixing catalysts to photothermal nanomaterials, in which the catalysts have a high activity only under mild conditions. Particularly, the method is useful in the case of using a catalyst substrate which is unstable at room temperature or the generation of a catalyst reactant which is unstable at room temperature. Additionally, a chemical catalyst or a heat-resistant enzyme capable of reacting at high temperatures and which save energy to allow the reaction to be carried out even at lower temperatures or room temperature by light irradiation.

Abstract translation: 本发明涉及使用光热纳米材料的光热特性来活化催化剂的方法，其中将光照射到催化剂 - 光热纳米材料络合物上以在催化剂具有低活性或没有活性的温度下活化催化剂。 根据本发明，活化催化剂的方法能够通过仅在整个反应介质的温度稍微改变的情况下仅增加纳米材料的周围的温度来活化催化剂。 根据该方法，可以进行催化反应，其通常在室温下显示高活性，即使在低温下也具有高活性。 也就是说，本发明可以提供使催化剂在低温和极冷区域具有高活性的方法，其中催化剂仅在温和条件下具有高活性的光热纳米材料。 特别地，该方法在使用在室温下不稳定的催化剂底物或在室温下不稳定的催化剂反应物的产生的情况下是有用的。 此外，能够在高温下反应并且能够即使在较低温度或室温下通过光照射也能够进行反应的化学催化剂或耐热酶。

公开(公告)号：WO2009116803A3

公开(公告)日：2009-12-23

申请号：PCT/KR2009001374

申请日：2009-03-18

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  SHIN YONG BEOM ,  CHUNG BONG HYUN ,  AHN JUN HYOUNG

Inventor： KIM MIN GON ,  SHIN YONG BEOM ,  CHUNG BONG HYUN ,  AHN JUN HYOUNG

IPC: G01N33/53

CPC classification number: G01N33/5438

Abstract: The invention relates to a biosensor capable of selectively measuring a trace amount of a sample and a production method therefor; and, more particularly, to a biosensor and a production method therefor, wherein the biosensor is produced by immobilizing, on a nano-electrode chip with electrical insulation, a receptor molecule that selectively bonds to a target substance, linking an enzyme thereto, and treating the linked enzyme with metal ions in order to deposit the metal ions on the surface of the nano-electrode. In an embodiment of the invention, a precipitate is produced on the surface of the nano-electrode by precipitation between the enzyme and the metal ions, and the nano-electrodes are then electrically connected to one another, thereby increasing electrical conductivity so as to make the invention useful for the quantitative analysis of target substances in trace amounts and/or of various concentrations.

Abstract translation: 本发明涉及能够选择性地测量痕量样品的生物传感器及其生产方法; 更具体地说，涉及一种生物传感器及其制造方法，其特征在于，所述生物传感器通过将具有电绝缘性的纳米电极芯片固定在选择性地键合到目标物质的受体分子上，将酶连接到其上， 连接的酶与金属离子以将金属离子沉积在纳米电极的表面上。 在本发明的一个实施方案中，通过酶和金属离子之间的沉淀在纳米电极的表面上产生沉淀，然后使纳米电极彼此电连接，从而增加导电性，从而使 本发明可用于定量分析微量和/或各种浓度的目标物质。

公开(公告)号：WO2012081940A3

公开(公告)日：2012-10-04

申请号：PCT/KR2011009746

申请日：2011-12-16

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  AHN JUN HYOUNG ,  SUNG YUN JU ,  SHIN YONG BEOM ,  JOUNG HYO ARM

Inventor： KIM MIN GON ,  AHN JUN HYOUNG ,  SUNG YUN JU ,  SHIN YONG BEOM ,  JOUNG HYO ARM

IPC: G01N27/333 ,  G01N33/48

CPC classification number: G01N33/5438 ,  G01N27/3278

Abstract: The present invention relates to a bio-molecule detection method in which an electrode is formed on a filtering membrane by screen printing to combine filtering characteristics of the membrane and signal measuring ability of the electrode. A membrane electrode according to the present invention provides a novel sensor in which the filtering function of the membrane and the signal measuring ability of the electrode are combined. According to the present invention, a target material is filtered by the membrane and measured, and a metal ion is reduced on the membrane to thereby increase electrical conductivity, with the result that the very small amount of target material may be detected with superior sensitivity using the amplified electrical signals, and quantitative analysis of the target material may be advantageously performed using the amplified electrical signals. In addition, a receptor material may be fixed on the aforementioned electrode, the thus-formed sample passes through the membrane to obtain only the target material that selectively binds to the receptor material, and the thus-obtained target material may be used in detecting electrical signals. The signal measurement of the sensor according to the present invention may use a variety of elements such as electrical conductivity and impedance.

Abstract translation: 本发明涉及一种生物分子检测方法，其中通过丝网印刷在滤膜上形成电极以组合膜的过滤特性和电极的信号测量能力。 根据本发明的膜电极提供了一种新颖的传感器，其中膜的过滤功能和电极的信号测量能力被组合。 根据本发明，通过膜对目标材料进行过滤并测量，并且在膜上还原金属离子，从而增加导电性，结果是可以以极高的灵敏度检测非常少量的目标材料， 放大的电信号和目标材料的定量分析可以有利地使用放大的电信号进行。 此外，受体材料可以固定在上述电极上，如此形成的样品通过膜以仅获得选择性地结合受体材料的靶材料，并且由此获得的靶材料可用于检测电气 信号。 根据本发明的传感器的信号测量可以使用诸如导电性和阻抗的各种元件。

公开(公告)号：WO2011065751A3

公开(公告)日：2011-11-10

申请号：PCT/KR2010008363

申请日：2010-11-24

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  SHIN YONG BEOM ,  O YOUNG KYENG ,  JOUNG HYO ARM

Inventor： KIM MIN GON ,  SHIN YONG BEOM ,  O YOUNG KYENG ,  JOUNG HYO ARM

IPC: G01N33/53 ,  G01N33/538 ,  G01N33/543

CPC classification number: G01N33/538 ,  G01N33/54366

Abstract: The present invention relates to a membrane sensor to which a porous film is attached and to a method for measuring immune reactions or enzyme reactions employing the same, and more specifically it relates to a membrane sensor in which a porous film is joined to the top of a membrane on which receptors are secured and to a method for measuring immune reactions or enzyme reactions employing the same. The present invention makes it possible to adjust the sensitivity of membrane biosensors by adjusting the hole size in the porous film and so makes it possible to measure analytes with a high degree of sensitivity by using just a small amount of sample, and makes it possible to simultaneously measure diverse types of analyte by attaching various types of receptor on the membrane sensor.

Abstract translation: 本发明涉及一种附着多孔膜的膜传感器和使用其的免疫反应或酶反应的测定方法，更具体地说，涉及一种膜传感器，其中多孔膜与 受体被固定的膜和用于测量免疫反应或使用其的酶反应的方法。 本发明通过调节多孔膜中的孔尺寸来调节膜生物传感器的灵敏度，因此可以通过仅使用少量的样品来测量具有高灵敏度的分析物，并且可以 同时通过在膜传感器上附加各种类型的受体来测量不同类型的分析物。

公开(公告)号：WO2011065747A3

公开(公告)日：2011-11-03

申请号：PCT/KR2010008356

申请日：2010-11-24

Applicant: KOREA RES INST OF BIOSCIENCE ,  KIM MIN GON ,  SHIN YONG BEOM ,  SUNG YOON JOO

Inventor： KIM MIN GON ,  SHIN YONG BEOM ,  SUNG YOON JOO

IPC: C12Q1/04 ,  B82B3/00 ,  G01N33/569

CPC classification number: G01N33/569 ,  B82Y15/00 ,  G01N33/54346

Abstract: The present invention relates to a rapid microorganism detection method using nanoparticles, and, more specifically, to a rapid microorganism detection method and a microorganism detection device wherein nanoparticles, on which are secured antibodies that specifically bind to a detection-target microorganism, are added to detection-target microorganisms so as to effect an immune reaction, the product of which is passed through a coagulation film so as to collect microorganisms in one place, and then the nanoparticles on which the antibodies are secured and the microorganisms which gave rise to the immune reaction are trapped on a trapping filtration membrane so as to measure whether there are microorganisms and the concentration thereof. By using nanoparticles on which are secured antibodies that specifically bind to a detection-target microorganism, the present invention can measure whether there are microorganisms and the concentration thereof in a fashion which is more economic and straightforward than with detection methods of the prior art, has outstanding sensitivity and is effective in measuring microorganisms in small amounts.

Abstract translation: 本发明涉及一种使用纳米粒子的快速微生物检测方法，更具体地说，涉及一种快速微生物检测方法和微生物检测装置，其中将纳米粒子（其上附着有与检测靶标微生物特异性结合的抗体） 检测对象微生物进行免疫反应，使其产物通过凝固膜以在一处收集微生物，然后将其上固定有抗体的纳米颗粒和产生免疫的微生物 反应被捕集在捕获过滤膜上以测量是否存在微生物及其浓度。 通过使用确保了与检测对象微生物特异性结合的抗体的纳米粒子，本发明能够以比现有技术的检测方法更经济，直接的方式测定是否存在微生物及其浓度， 突出的灵敏度，并能有效测量少量微生物。

公开(公告)号：WO2011090262A2

公开(公告)日：2011-07-28

申请号：PCT/KR2010007902

申请日：2010-11-10

Applicant: KOREA RES INST OF BIOSCIENCE ,  SHIN YONG BEOM ,  LEE SEUNG WOO

Inventor： SHIN YONG BEOM ,  LEE SEUNG WOO

IPC: H01L21/027

CPC classification number: H01L21/0272 ,  B82Y20/00 ,  G02B2207/101 ,  G03F7/0035 ,  G03F7/40

Abstract: The present invention relates to a lithography method using tilted evaporation, and includes: (1) coating a resist on top of a substrate; (2) patterning the resist using a lithography process; (3) tilt-evaporating a first thin film material on an upper layer of the patterned resist to form a modified pattern mask; (4) evaporating a second thin film material on the top of the substrate with the modified mask pattern; and (5) removing the resist coated on the top of the substrate.

Abstract translation: 本发明涉及使用倾斜蒸发的光刻方法，并且包括：（1）在基板的顶部涂覆抗蚀剂; （2）使用光刻工艺构图抗蚀剂; （3）在图案化的抗蚀剂的上层上倾斜蒸发第一薄膜材料以形成改性图案掩模; （4）用修改后的掩模图案在衬底的顶部上蒸发第二薄膜材料; 和（5）去除涂覆在基材顶部上的抗蚀剂。

公开(公告)号：EP2506012A4

公开(公告)日：2014-01-15

申请号：EP10833563

申请日：2010-11-24

Applicant: KOREA RES INST OF BIOSCIENCE ,  INFOPIA CO LTD

Inventor： KIM MIN GON ,  SHIN YONG BEOM ,  O YOUNG KYENG ,  JOUNG HYO ARM

IPC: G01N33/53 ,  G01N33/52 ,  G01N33/538 ,  G01N33/543

CPC classification number: G01N33/538 ,  G01N33/54366