中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

25 records (took 0.023 seconds)

    IN-GEL TAGGING AND IN-GEL DIGESTION FOR PHOSPHOPROTEINS ANALYSIS AND PHOSPHORYLATION SITE IDENTIFICATION
    1.
    发明申请
    IN-GEL TAGGING AND IN-GEL DIGESTION FOR PHOSPHOPROTEINS ANALYSIS AND PHOSPHORYLATION SITE IDENTIFICATION 审中-公开
    磷酸化分析和磷酸化位点识别的凝胶标记和凝胶消解

    公开(公告)号：WO2006028310A1

    公开(公告)日：2006-03-16

    申请号：PCT/KR2004/002577

    申请日：2004-10-08

    Applicant: KOREA BASIC SCIENCE INSTITUTE ,  AHN, Yeong Hee ,  YOO, Jong Shin ,  LEE, Jae Yong ,  KIM, Jin Young ,  CHO, Kun

    Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  LEE, Jae Yong ,  KIM, Jin Young ,  CHO, Kun

    IPC: C12Q1/37

    CPC classification number: G01N33/6842 ,  G01N33/6848

    Abstract: The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.

    Abstract translation: 本发明涉及用位点特异性标记试剂对磷酸化蛋白质进行磷酸化位点特异性标记的方法，并分析得到的标记物，更具体地说，一种用于原位标记保留在聚合物凝胶中的磷酸化蛋白的磷酸化位点的方法， 亲核标记试剂。 它还涉及通过适当选择亲核标记试剂在以前的磷酸化位点产生新的蛋白水解可切割位点的方法。 它还涉及通过凝胶内消化先前凝胶内标记蛋白的磷酸肽分析和磷酸化位点鉴定的方法以及随后对所得肽的质量分析。 本发明提供了凝胶化学标记方法，用于保留在聚合物凝胶基质中的磷酸蛋白的磷酸氨基酸残基。 磷酸蛋白可以通过各种方法如凝胶电泳固定在凝胶基质中。 在磷酸化蛋白质的磷酸化氨基酸残基的标签反应期间，将固定的磷酸蛋白质保留在凝胶基质中，并且所得到的标记蛋白质也保留在凝胶基质中，直到完成标记试剂的洗涤之后的纯化步骤。 标记的蛋白质被蛋白酶消化，并将得到的消化肽从凝胶释放到溶液中并用于肽质量分析。

    METHOD FOR TAGGING OF CARBOHYDRATES WITH ACTIVE METHYLENE COMPOUND
    2.
    发明申请
    METHOD FOR TAGGING OF CARBOHYDRATES WITH ACTIVE METHYLENE COMPOUND 审中-公开
    用活性亚甲基化合物标记碳酸氢盐的方法

    公开(公告)号：WO2005016948A1

    公开(公告)日：2005-02-24

    申请号：PCT/KR2003/001654

    申请日：2003-08-14

    Applicant: KOREA BASIC SCIENCE INSTITUTE ,  AHN, Yeong Hee ,  YOO, Jong Shin ,  KIM, Soohyun

    Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  KIM, Soohyun

    IPC: C07H1/00

    CPC classification number: C07H1/00

    Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.

    Abstract translation: 本发明涉及用活性亚甲基化合物标记碳水化合物的方法。 特别涉及用活性亚甲基化合物标记碳水化合物的方法，包括制备碳水化合物缀合物的步骤，其中碳水化合物和亚甲基化合物通过混合碳水化合物混合物和亚甲基化合物在含有胺基催化剂的极性非质子溶剂的水溶液下混合。 本发明的标记方法不需要多种化学试剂，即使在存在一定量的杂质的情况下也可以进行反应。 因此，可用于各种样品中存在的寡糖的分析。

    AN ADDITIVE SCORING METHOD FOR MODIFIED POLYPEPTIDE
    5.
    发明申请
    AN ADDITIVE SCORING METHOD FOR MODIFIED POLYPEPTIDE 审中-公开
    改性多糖的添加剂分选方法

    公开(公告)号：WO2007018327A1

    公开(公告)日：2007-02-15

    申请号：PCT/KR2005/003037

    申请日：2005-09-14

    Applicant: KOREA BASIC SCIENCE INSTITUTE ,  AHN, Yeong Hee ,  KWON, Kyung-Hoon ,  YOO, Jong Shin

    Inventor： AHN, Yeong Hee ,  KWON, Kyung-Hoon ,  YOO, Jong Shin

    IPC: G01N33/68

    CPC classification number: C07K14/4732

    Abstract: The present invention relates to a method for analyzing modified polypeptide sequence and modified information thereof. More precisely, the invention relates to a method for identifying modified peptide in which fragment ion signals are obtained from target peptide precursor by using tandem mass spectrometry, candidate peptides and every possible peptide fragmentation patterns thereof are designed based on the mass data thereby, match scores corresponding to each fragmentation pattern of candidate peptides are given, and then by combining each matching scores derived from possible fragmentation patterns of a candidate peptide, modified target polypeptide is identified by tandem mass data based on the provided match score. The scoring method for modified polypeptide of the present invention can greatly contribute to increasing searching efficiency of modified peptides and reliability of the searching results by integrating all scored values obtained from different fragmentation patterns of a candidate peptide.

    Abstract translation: 本发明涉及一种分析修饰多肽序列的方法及其修饰信息。 更准确地说，本发明涉及一种用于鉴定通过使用串联质谱法从靶肽前体获得片段离子信号的修饰肽的方法，并且基于质量数据设计候选肽和其每种可能的肽断裂模式，从而匹配得分 对应于候选肽的每个断裂模式，然后通过组合从候选肽的可能的断裂模式导出的每个匹配分数，基于所提供的匹配分数，通过串联质量数据鉴定修饰的目标多肽。 本发明的修饰多肽的评分方法可以通过整合从候选肽的不同片段化模式获得的所有得分值，大大有助于提高修饰肽的搜索效率和搜索结果的可靠性。

    SELECTIVE LABELING AGENT FOR PHOSPHOPROTEOME ANALYSIS AND PHOSPHORYLATED SITE ANALYSIS
    7.
    发明申请
    SELECTIVE LABELING AGENT FOR PHOSPHOPROTEOME ANALYSIS AND PHOSPHORYLATED SITE ANALYSIS 审中-公开
    选择性标记物质用于磷酸盐分析和磷酸盐化位点分析

    公开(公告)号：WO2005075993A1

    公开(公告)日：2005-08-18

    申请号：PCT/KR2004/000256

    申请日：2004-02-10

    Applicant: KOREA BASIC SCIENCE INSTITUTE ,  AHN, Yeong Hee ,  YOO, Jong Shin ,  KIM, Jin Young ,  CHO, Kun

    Inventor： AHN, Yeong Hee ,  YOO, Jong Shin ,  KIM, Jin Young ,  CHO, Kun

    IPC: G01N33/53

    CPC classification number: G01N33/6848 ,  A61K31/155 ,  G01N33/6803 ,  G01N33/6842 ,  G01N2458/15

    Abstract: Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag having a R-L-G moiety wherein R is a nucleophilic functional group that selectively bind with dephosphorylated amino acid residues, G is selected from the group consisting of guanidine moiety or protected guanidine moiety such as a mono-N-protected guanidino group, a di-N,N'-protected guanidino group and an N'-protected guanidino group, and L is a linker linking the R and the G; and 3) subjecting the tagged proteins or peptides to mass spectrometry. The method is capable of precisely analyzing mass of phosphoproteins of trace amounts as well as positions of phosphoryated amino acids.

    Abstract translation: 公开了一种分析磷蛋白或磷酸肽的质量并分析磷蛋白或磷酸肽的磷酸化位置的方法，包括以下步骤：1）使磷酸蛋白或磷酸肽的至少一个Ser和/或Thr残基去磷酸化; 2）用具有RLG部分的标签标记去磷酸化的氨基酸残基，其中R是与去磷酸化的氨基酸残基选择性结合的亲核官能团，G选自胍部分或被保护的胍部分，例如单 - N-保护的胍基，二-N，N'保护的胍基和N'保护的胍基，L是连接R和G的连接基团; 和3）对标记的蛋白质或肽进行质谱分析。 该方法能够精确分析痕量的磷蛋白质量以及磷酸化氨基酸的位置。

    APPARATUS FOR VISUALIZING AND ANALYZING GENE EXPRESSION PATTERNS USING GENE ONTOLOGY TREE AND METHOD THEREOF
    9.
    发明申请
    APPARATUS FOR VISUALIZING AND ANALYZING GENE EXPRESSION PATTERNS USING GENE ONTOLOGY TREE AND METHOD THEREOF 审中-公开
    用于使用基因本体树观察和分析基因表达模式的装置及其方法

    公开(公告)号：WO2010018882A1

    公开(公告)日：2010-02-18

    申请号：PCT/KR2008/004735

    申请日：2008-08-14

    Applicant: KOREA BASIC SCIENCE INSTITUTE ,  KWON, Kyung-Hoon ,  PARK, Gun Wook ,  LEE, Jeong Hwa ,  YOO, Jong Shin

    Inventor： KWON, Kyung-Hoon ,  PARK, Gun Wook ,  LEE, Jeong Hwa ,  YOO, Jong Shin

    IPC: G06F17/30 ,  C12Q1/68

    CPC classification number: G06F19/28 ,  G06F19/20 ,  G06F19/26

    Abstract: The present invention relates to an apparatus for visualizing or analyzing gene expression patterns of a biological sample using gene ontology tree and a method of the same, more precisely, an apparatus for visualizing or analyzing gene expression patterns which are regarded as biologically important using gene ontology tree by calculating complexity and a method of the same. This apparatus of the present invention is useful for comparing gene expression patterns in different biological samples using data produced by the said apparatus and the method of the present invention. According to the present invention, gene expression patterns in relation to molecular functions, biological processes or cellular components can be analyzed by investigating protein or RNA expression in biological samples. So, the apparatus and the method of the present invention can be effectively used for analysis of biologically important aspects such as functional changes, evolutionary stages or developmental stages of each biological sample.

    Abstract translation: 本发明涉及使用基因本体树来观察或分析生物样品的基因表达模式的装置及其方法，更确切地说，涉及用于使用基因本体论被视为生物学重要性的基因表达模式进行可视化或分析的装置 树的计算复杂度和方法相同。 本发明的该装置可用于使用由所述装置产生的数据和本发明的方法来比较不同生物样品中的基因表达模式。 根据本发明，可以通过研究生物样品中的蛋白质或RNA表达来分析与分子功能，生物过程或细胞成分有关的基因表达模式。 因此，本发明的装置和方法可以有效地用于生物学重要方面的分析，例如每个生物样品的功能变化，进化阶段或发育阶段。

Patent Agency Ranking