公开(公告)号：US20060160199A1

公开(公告)日：2006-07-20

申请号：US11313541

申请日：2005-12-21

Applicant: Robert DiCosimo ,  Mark Payne ,  Anna Panova ,  Jeffrey Thompson ,  Daniel O'Keefe

Inventor： Robert DiCosimo ,  Mark Payne ,  Anna Panova ,  Jeffrey Thompson ,  Daniel O'Keefe

IPC: C12P7/42 ,  C12N9/80

CPC classification number: C12N9/78 ,  C12P7/42

Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.

公开(公告)号：US20060115883A1

公开(公告)日：2006-06-01

申请号：US11338471

申请日：2006-01-24

Applicant: Michael Bramucci ,  Robert DiCosimo ,  John Gavagan ,  Mark Payne ,  Robert Fallon ,  Sarita Chauhan

Inventor： Michael Bramucci ,  Robert DiCosimo ,  John Gavagan ,  Mark Payne ,  Robert Fallon ,  Sarita Chauhan

IPC: C12P7/40 ,  C12N1/20

CPC classification number: C12N9/88 ,  C12N9/80 ,  C12P7/40 ,  C12P41/006

Abstract: A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.

Abstract translation: 已经开发了一种由（E，Z）-2-甲基-2-丁烯腈（2M 2 BN）的混合物通过区域选择性制备（E） - 和（Z）-2-甲基-2-丁烯酸（2M 2 BA）的方法 使用具有腈水解酶活性或腈水合酶和酰胺酶活性的组合的酶催化剂将（E）-2M2BN水解成（E）-2-甲基-2-丁烯酸（2M2BA）。 该方法提供高产率而不显着转化（Z）-2M2BN至（Z）-2M2BA。 （E）-2M2BN至（E）-2M2BA的区域选择性水解使（E）-2M2BA与（Z）-2M2BN或（Z）-2-甲基-2-丁烯酰胺（2M2BAm）的容易分离成为可能， 随后将（Z）-2M2BN或（Z）-2M2BAm转化为（Z）-2M2BA。

公开(公告)号：US06562603B2

公开(公告)日：2003-05-13

申请号：US09921260

申请日：2001-08-02

Applicant: Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

Inventor： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

IPC: C12P742

CPC classification number: C12P7/625 ,  C08G63/06 ,  C08G63/85 ,  C12P7/42 ,  C12R1/01 ,  C12R1/40

Abstract: This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for &egr;-caprolactone in the preparation of highly branched copolyester.

Abstract translation: 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 在制备高支化共聚酯时，使用3-羟基戊酸作为ε-己内酯的替代物。

公开(公告)号：US06551804B2

公开(公告)日：2003-04-22

申请号：US09766955

申请日：2001-01-22

Applicant: Robert DiCosimo ,  Robert D. Fallon ,  John E. Gavagan

Inventor： Robert DiCosimo ,  Robert D. Fallon ,  John E. Gavagan

IPC: C12P1300

CPC classification number: C12N1/04 ,  C12N9/78 ,  C12N9/96 ,  C12N11/04 ,  C12N11/08 ,  C12N11/10 ,  C12P7/40 ,  C12P13/002 ,  Y02P20/588

Abstract: This invention relates to an improved process for preparing a carboxylic acid from the corresponding nitrile using a nitrilase catalyst. More particularly, the instant invention converts 2-methylglutaronitrile to 4-cyanopentanoic acid in aqueous solution using an enzyme catalyst having an nitrilase activity, immobilized in alginate, and crosslinked with glutaraldehyde and polyethylenimine.

Abstract translation: 本发明涉及使用腈水解酶催化剂从相应的腈制备羧酸的改进方法。 更具体地，本发明使用具有腈水解酶活性的酶催化剂将2-甲基戊二酸转化成水溶液中的4-氰基戊酸，固定在藻酸盐中，并与戊二醛和聚乙烯亚胺交联。

公开(公告)号：US06455730B1

公开(公告)日：2002-09-24

申请号：US09632419

申请日：2000-08-04

Applicant: Sarita Chauhan ,  Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

Inventor： Sarita Chauhan ,  Robert Dicosimo ,  Robert D. Fallon ,  John E. Gavagan ,  Mark S. Payne

IPC: C07C5108

CPC classification number: C12P7/62 ,  C12N9/78 ,  C12P7/44

Abstract: This invention relates to a process for preparing dicarboxylic acid monoesters from cyanocarboxylic acid esters. More particularly, using the nitrilase from Acidovorax facilis 72W (ATCC 55745) in various forms as an enzyme catalyst, an aliphatic or aromatic cyanocarboxylic acid ester is converted to the corresponding dicarboxylic acid monoester with high chemoselectivity at 100% conversion. An embodiment of the invention also provides a process for obtaining high chemoselectively and high regioselectivity.

Abstract translation: 本发明涉及由氰基羧酸酯制备二羧酸单酯的方法。 更具体地，使用来自Acidovorax facilis 72W（ATCC 55745）的腈水解酶以各种形式作为酶催化剂，将脂族或芳族氰基羧酸酯在100％转化率下转化为具有高化学选择性的相应的二羧酸单酯。 本发明的实施方案还提供了获得高化学选择性和高区域选择性的方法。

公开(公告)号：US06251646B1

公开(公告)日：2001-06-26

申请号：US09352015

申请日：1999-07-12

Applicant: Robert Dicosimo ,  Arie Ben-Bassat ,  Robert D. Fallon

Inventor： Robert Dicosimo ,  Arie Ben-Bassat ,  Robert D. Fallon

IPC: C12N900

CPC classification number: C12P13/00 ,  C12N1/04 ,  C12N9/78 ,  C12N9/96 ,  C12N11/04 ,  C12N11/08 ,  C12N11/10

Abstract: A method for the stabilization of nitrilase activity of unimmobilized or immobilized microbial cells has been developed. The unimmobilized or immobilized microbial cells are stored in an aqueous solution containing from 0.100 M to the saturation concentration of an inorganic salt of bicarbonate or carbonate, including ammonium, sodium and potassium salts of bicarbonate or carbonate. Aqueous suspensions containing at least 100 mM bicarbonate or carbonate limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), and Acidovorax facilis 72W (ATCC 55746).

Abstract translation: 已经开发了用于稳定未固定或固定的微生物细胞的腈水解酶活性的方法。 将未固定或固定的微生物细胞储存在含有0.100M的水溶液中，使饱和浓度为碳酸氢盐或碳酸盐的无机盐，包括碳酸氢盐或碳酸盐的铵，钠和钾盐。 含有至少100mM碳酸氢盐或碳酸氢盐的水性悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固化或固定的细胞的所需腈水解酶活性。 特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745）和Acidovorax facilis 72W（ATCC 55746）。

公开(公告)号：US5928933A

公开(公告)日：1999-07-27

申请号：US105713

申请日：1998-06-26

Applicant: Robert Dicosimo ,  Wonpyo Hong

Inventor： Robert Dicosimo ,  Wonpyo Hong

IPC: C12P13/04 ,  C12P13/24 ,  C12P41/00 ,  C07C1/04

CPC classification number: C12P41/005 ,  C12P13/04 ,  C12P13/24 ,  Y10S435/921

Abstract: The present invention relates to a process for the enzymatic resolution of racemic mixtures of N-(alkoxycarbonyl)-4-keto-D,L-proline alkyl esters, using Candida antarctica lipase fraction B (CALB) as enzyme catalyst to enantioselectively hydrolyze the alkyl ester of one of the two enantiomers present. Separating the unreacted N-(alkoxycarbonyl)-4-keto-D-proline alkyl ester from the N-(alkoxycarbonyl)-4-keto-L-proline, followed by hydrogenation of the keto group of the D-isomer and subsequent hydrolysis of the ester and N-(alkoxy-carbonyl) groups produces cis-4-hydroxy-D-proline in high yield. Diastereomeric mixtures of N-(alkoxycarbonyl)-4-hydroxyproline alkyl esters can also be resolved using CALB to ultimately produce cis-4-hydroxy-D-proline or trans-4-hydroxy-L-proline.

Abstract translation: 本发明涉及使用南极假丝酵母脂肪酶B（CALB）作为酶催化剂来解离N-（烷氧基羰基）-4-酮基-D-脯氨酸烷基酯的外消旋混合物的方法，以对映体水解烷基 存在两种对映体之一的酯。 从N-（烷氧基羰基）-4-酮-L-脯氨酸分离未反应的N-（烷氧基羰基）-4-酮基-D-脯氨酸烷基酯，然后氢化D-异构体的酮基，随后水解 酯和N-（烷氧基 - 羰基）基团以高产率产生顺式-4-羟基-D-脯氨酸。 N-（烷氧基羰基）-4-羟基脯氨酸烷基酯的非对映异构体混合物也可以使用CALB来分解，以最终产生顺式-4-羟基-D-脯氨酸或反式-4-羟基-L-脯氨酸。

公开(公告)号：US5834262A

公开(公告)日：1998-11-10

申请号：US606144

申请日：1996-02-23

Applicant: David Leroy Anton ,  Robert DiCosimo

Inventor： David Leroy Anton ,  Robert DiCosimo

IPC: C12N9/04 ,  C12N9/08 ,  C12P7/40 ,  C12N15/53

CPC classification number: C12N9/0065 ,  C12N9/0006 ,  C12P7/40

Abstract: An improved process for preparing glyoxylic acid comprising using the enzyme glycolate oxidase in the form of permeabilized microbial cell transformants selected from Aspergillus nidulans, Hansenula polymorpha, Pichia pastoris and Escherichia coli to oxidize glycolic acid with oxygen in an aqueous solution that includes an amine.

Abstract translation: 一种用于制备乙醛酸的改进方法，包括使用选自构巢曲霉，多形汉逊酵母，巴斯德毕赤酵母和大肠杆菌的透化微生物细胞转化体形式的乙醇酸氧化酶酶，以在含有胺的水溶液中氧化乙醇酸。

公开(公告)号：US4876348A

公开(公告)日：1989-10-24

申请号：US792421

申请日：1985-10-29

Applicant: Robert DiCosimo ,  James D. Burrington ,  Dev D. Suresh

Inventor： Robert DiCosimo ,  James D. Burrington ,  Dev D. Suresh

IPC: C07D213/84

CPC classification number: C07D213/84

Abstract: Disclosed is a process comprising (1) dehydrocyclization of 2-methylglutaronitrile over Pt or Pd based catalysts to make a mixture containing 3-methylpyridine and lesser amounts of 3-methylpiperidine and (2) reacting such a mixture with NH.sub.3 and O.sub.2 over certain oxide catalysts containing P, V and Mo to obtain 3-cyanopyridine.

Abstract translation: 公开了一种方法，其包括（1）通过Pt或Pd基催化剂使2-甲基戊二腈脱氢环化以制备含有3-甲基吡啶和较少量的3-甲基哌啶的混合物，和（2）在某些氧化物催化剂上使这种混合物与NH 3和O 2反应 含有P，V和Mo，得到3-氰基吡啶。

公开(公告)号：US4783550A

公开(公告)日：1988-11-08

申请号：US750552

申请日：1985-07-01

Applicant: Robert DiCosimo ,  Hsiao-Chiung Szabo

Inventor： Robert DiCosimo ,  Hsiao-Chiung Szabo

IPC: C07C67/05

CPC classification number: C07C67/05

Abstract: Benzene is oxidized to produce the phenyl ester of a perfluoromonocarboxylic acid by reaction with H.sub.2 O.sub.2, a perfluoro acid or a combination of acetaldehyde and molecular oxygen, in the presence of (1) a cobalt salt of an organic acid catalyst and (2) a perfluoromonocarboxylic acid solvent.

Abstract translation: 在（1）有机酸催化剂的钴盐和（2）全氟单体的存在下，通过与H 2 O 2，全氟酸或乙醛和分子氧的组合反应，氧被氧化生成全氟单体羧酸的苯基酯 酸溶剂。