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    Reducing non-specific enzyme binding to enhance lignocellulose conversion
    112.
    发明授权
    Reducing non-specific enzyme binding to enhance lignocellulose conversion 有权
    减少非特异性酶结合增强木质纤维素的转化

    公开(公告)号：US09074231B2

    公开(公告)日：2015-07-07

    申请号：US13652267

    申请日：2012-10-15

    Applicant: The United States of America as Represented by the Secretary of Agriculture

    Inventor： JunYong Zhu

    IPC: C12P19/02 ,  C12P7/10 ,  C12P19/14

    CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  Y02E50/16 ,  Y02E50/343

    Abstract: A system is provided for reducing non-specific binding of an enzyme to lignin to enhance an enzymatic processing of a lignocellulosic material. The enhancements provide economic and process advantages to any process that converts a lignocellulosic biomass into a product using an enzyme. Systems are provided comprising a reaction vessel; a lignocellulosic feedstock comprising a component selected from the group consisting of a hardwood, a softwood, or a non-wood material; an enzyme component including a cellulase, a hemicellulase, or a combination thereof; and, water. The reaction vessel can contain a combination of the lignocellulosic feedstock, the water, and the enzyme component at a pH ranging from about 5.2 to about 6.2; and, the lignocellulosic feedstock can be saccharified in the reaction vessel. Moreover, the systems can include a lignosulfonate, with or without a pH of about 5.2 to about 6.2, to also reduce non-specific binding and enhance enzymatic activity.

    Abstract translation: 提供了一种用于减少酶与木质素的非特异性结合以增强木质纤维素材料的酶加工的系统。 该增强为使用酶将木质纤维素生物质转化成产物的任何方法提供经济和工艺优点。 提供了包括反应容器的系统; 木质纤维素原料，其包含选自硬木，软木或非木材的组分; 包括纤维素酶，半纤维素酶或其组合的酶组分; 和水。 反应容器可以在约5.2至约6.2的pH范围内包含木质纤维素原料，水和酶组分的组合; 并且木质纤维素原料可以在反应容器中进行糖化。 此外，该系统可以包括具有或不具有约5.2至约6.2的pH的木质素磺酸盐，以减少非特异性结合并增强酶活性。

    PROCESSES AND APPARATUS FOR PRODUCING FERMENTABLE SUGARS FROM BIOMASS BY HOT-WATER EXTRACTION AND ENZYMATIC HYDROLYSIS
    113.
    发明申请
    PROCESSES AND APPARATUS FOR PRODUCING FERMENTABLE SUGARS FROM BIOMASS BY HOT-WATER EXTRACTION AND ENZYMATIC HYDROLYSIS 审中-公开
    通过热水提取和酶水解从生物量生产可食用糖的工艺和设备

    公开(公告)号：US20150184259A1

    公开(公告)日：2015-07-02

    申请号：US14583566

    申请日：2014-12-26

    Applicant: API Intellectual Property Holdings, LLC

    Inventor： Ryan ZEBROSKI ,  James SCHULTZE

    IPC: C13K1/02 ,  C12P19/02 ,  C13K1/04 ,  C12P7/10 ,  C12P19/14

    CPC classification number: C13K1/02 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C13K1/04 ,  Y02E50/16

    Abstract: The present invention is capable of producing glucose and hemicellulose sugars from lignocellulosic biomass. In some variations, a process includes optionally pre-steaming a biomass feedstock; extracting the feedstock with liquid hot water to produce glucan-rich solids and an extract liquor containing dissolved solids, which include hemicellulosic oligomers and lignin; washing the glucan-rich solids; hydrolyzing the hemicellulosic oligomers by contacting the extract liquor with an acid catalyst or enzymes possessing hemicellulase activity; separately hydrolyzing the glucan by contacting the glucan-rich solids stream with an acid catalyst or enzymes possessing glucanase activity, optionally with removing the glucose in situ by microfiltration and/or ultrafiltration; and recovering or fermenting each of the hemicellulosic monomers and the glucose. Preferred configurations and conditions are disclosed.

    Abstract translation: 本发明能够从木质纤维素生物质生产葡萄糖和半纤维素糖。 在一些变型中，方法包括任选地预蒸汽生物质原料; 用液体热水提取原料以产生富含葡聚糖的固体，以及含有溶解的固体的提取液，包括半纤维素低聚物和木质素; 洗涤富含葡聚糖的固体; 通过使萃取液与酸催化剂或具有半纤维素酶活性的酶接触来水解半纤维素低聚物; 通过使富含葡聚糖的固体物流与酸催化剂或具有葡聚糖酶活性的酶接触来分别水解葡聚糖，任选地通过微量过滤和/或超滤原位除去葡萄糖; 并回收或发酵每种半纤维素单体和葡萄糖。 公开了优选的配置和条件。

    Advanced Methods for Sugar Production from Lignocellulosic Biomass and Fermenting Sugars to Microbial Lipids
    114.
    发明申请
    Advanced Methods for Sugar Production from Lignocellulosic Biomass and Fermenting Sugars to Microbial Lipids 审中-公开
    从木质纤维素生物质和发酵糖到微生物脂质的糖生产的高级方法

    公开(公告)号：US20150184212A1

    公开(公告)日：2015-07-02

    申请号：US14410769

    申请日：2013-06-25

    Applicant: Washington State University

    Inventor： Mahesh Bule ,  Allan Gao ,  Shulin Chen

    IPC: C12P19/14 ,  D21C1/08 ,  D21C11/10 ,  C12P19/02

    CPC classification number: C12P19/14 ,  C10L1/026 ,  C12P19/02 ,  C12P2201/00 ,  D21C1/08 ,  D21C11/10 ,  Y02E50/13 ,  Y02P20/582

    Abstract: Methods for facilitating sugar release from lignocellulosic biomass and for utilizing the sugars for microbial lipid (e.g. biofuel) production are provided. The methods involve pretreating lignocellulosic biomass using various oxidizing agents (ozone, peroxone, etc.) at a temperature not higher than 50° C. and pressure no higher than 1.5 atm to render the biomass more accessible to enzymatic hydrolytic degradation into sugars and utilizing soluble sugars for fermenting oleaginous microorganism to produce microbial lipids.

    Abstract translation: 提供了促进从木质纤维素生物质释放糖并且用于利用糖用于微生物脂质（例如生物燃料）生产的方法。 所述方法包括使用各种氧化剂（臭氧，过氧化物等）在不高于50℃和不高于1.5大气压的温度下预处理木质纤维素生物质，以使生物质更易于酶水解降解成糖并利用可溶性 用于发酵含油微生物以产生微生物脂质的糖。

    METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION
    117.
    发明申请
    METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 审中-公开
    使用来自青蒿素的ALPHA-AMYLASE进行分离的方法

    公开(公告)号：US20150152442A1

    公开(公告)日：2015-06-04

    申请号：US14561085

    申请日：2014-12-04

    Applicant: DANISCO US INC.

    Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

    IPC: C12P7/14 ,  C12C7/04

    CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

    Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

    Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

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