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    Light-switchable gene expression system and the methods for controlling gene expression in prokaryotic bacterium
    131.
    发明授权
    Light-switchable gene expression system and the methods for controlling gene expression in prokaryotic bacterium 有权

    公开(公告)号：US10184126B2

    公开(公告)日：2019-01-22

    申请号：US14419106

    申请日：2013-08-01

    Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

    Inventor： Yi Yang ,  Xianjun Chen ,  Zhengcai Ma ,  Renmei Liu

    IPC: C12N15/62 ,  C12N15/70 ,  C12N15/74 ,  C12N15/63 ,  C12N15/75 ,  C12P21/02 ,  C12N9/02 ,  C12N13/00

    Abstract: Provided is an optically controlled gene expression system of prokaryotic bacterium, comprising: a) a photosensitive recombinant transcription factor encoding gene, the photosensitive recombinant transcription factor is one fusion protein comprising a first polypeptide as the DNA bonding domain and a second polypeptide as the photosensitive domain; b) a target transcription unit comprising promoter or promoter-reaction element or reaction element-promoter containing at least one reaction element recognized/bound by the first polypeptide and the nucleic acid sequence to be transcribed. Also provided is a prokaryotic expression vector comprising said optically controlled gene expression system, and a method for regulating gene expression in a prokaryotic host cell by using the optically controlled gene expression system. Also provided is a reagent kit containing different components of the optically controlled gene expression system. The optically controlled gene expression system of prokaryotic bacterium has a quick, effective and powerful induction, is safer than other inducers, is of little or no toxicity, and can control gene expression both spatially and temporally, and can regulate many life processes of prokaryotic bacterium.

    CATALYSTS, METHODS, AND SYSTEMS FOR PREPARING CARBAMATES
    139.
    发明申请
    CATALYSTS, METHODS, AND SYSTEMS FOR PREPARING CARBAMATES 有权
    催化剂，制备碳酸盐的方法和系统

    公开(公告)号：US20170015622A1

    公开(公告)日：2017-01-19

    申请号：US15124397

    申请日：2014-03-13

    Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

    Inventor： Zhenshan HOU ,  Ran ZHANG

    IPC: C07C269/04 ,  B01J23/14 ,  B01J23/34 ,  B01J23/10 ,  B01J23/83

    CPC classification number: C07C269/04 ,  B01J21/00 ,  B01J23/10 ,  B01J23/14 ,  B01J23/34 ,  B01J23/83 ,  B01J23/92 ,  B01J23/94 ,  B01J2523/31 ,  B01J2523/3712 ,  B01J2523/60 ,  Y02P20/584 ,  C07C271/12

    Abstract: Methods, systems and kits for preparing carbamates as well as catalysts for preparing the carbamates, are disclosed. The methods for preparing carbamate can include providing a catalyst comprising cerium oxide (CeO2) and at least one metal selected from the group consisting of iron (Fe), manganese (Mn), titanium (Ti), cobalt (Co), aluminum (Al), zinc (Zn), calcium (Ca), tin (Sn), indium (In), and any combination thereof; contacting the catalyst with at least one amine and at least one alcohol to form a mixture; and contacting the mixture with carbon dioxide under conditions sufficient to form the carbamate.

    Abstract translation: 公开了用于制备氨基甲酸酯的方法，系统和试剂盒以及用于制备氨基甲酸酯的催化剂。 制备氨基甲酸酯的方法可以包括提供包含氧化铈（CeO 2）和至少一种选自铁（Fe），锰（Mn），钛（Ti），钴（Co），铝（Al ），锌（Zn），钙（Ca），锡（Sn），铟（In）及其任何组合; 使催化剂与至少一种胺和至少一种醇接触以形成混合物; 并在足以形成氨基甲酸酯的条件下将混合物与二氧化碳接触。

    A METHOD OF MEASUREMENT AND DETERMINATION ON FRACTURE TOUGHNESS OF STRUCTURAL MATERIALS AT HIGH TEMPERATURE
    140.
    发明申请
    A METHOD OF MEASUREMENT AND DETERMINATION ON FRACTURE TOUGHNESS OF STRUCTURAL MATERIALS AT HIGH TEMPERATURE 有权
    高温结构材料断裂韧性测量与测定方法

    公开(公告)号：US20160299046A1

    公开(公告)日：2016-10-13

    申请号：US14915659

    申请日：2014-09-25

    Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY ,  SHANGHAI ELECTRIC POWER EQUIPMENT CO., LTD. SHANGHAI STEAM TURBINE FACTORY

    Inventor： Fuzhen XUAN ,  Haitao WANG ,  Qiongqi WANG ,  Linbo MEI ,  Xia LIU ,  Yuhui HUANG

    IPC: G01N3/18 ,  G01N3/28

    CPC classification number: G01N3/18 ,  G01N3/00 ,  G01N3/08 ,  G01N3/28 ,  G01N2203/0017 ,  G01N2203/006 ,  G01N2203/0069

    Abstract: Disclosed is a method of measurement and determination on fracture toughness of structural materials at high temperature, comprising: preliminary assessing the ductility of a material based on a high-temperature uniaxial tensile test and the fracture characteristic; designing and manufacturing a CT specimen; conducting a monotonic loading fracture test on the CT specimen at high temperature; modifying a load-displacement curve output by a testing machine; determining a passivation coefficient M for the crack of the structural material; reversely recursing instant load-displacement data pairs corresponding to the instant crack length; calculating a J_R crack extension resistance curve of the tensile test; examining the validity of the J_R crack extension resistance curve and the fracture toughness JIC; calculating the fracture toughness per equivalent of the structural material KIC. The present invention overcomes the difficulty of placing an extensometer inside a high-temperature furnace.

    Abstract translation: 本发明公开了一种高温结构材料的断裂韧性的测定方法，其特征在于，包括：基于高温单轴拉伸试验和断裂特性初步评价材料的延展性; 设计制造CT样本; 在高温下对CT标本进行单调载荷断裂试验; 修改由试验机输出的载荷 - 位移曲线; 确定结构材料裂纹的钝化系数M; 对应于即时裂纹长度的反向递归瞬时载荷 - 位移数据对; 计算拉伸试验的J_R裂纹扩展电阻曲线; 检查J_R裂纹扩展阻力曲线和断裂韧性JIC的有效性; 计算每个等效结构材料KIC的断裂韧性。 本发明克服了将引伸计置于高温炉内的困难。

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