公开(公告)号：US20050179894A1

公开(公告)日：2005-08-18

申请号：US10777720

申请日：2004-02-13

Applicant: Jiann-Hua Wang ,  Hui Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

Inventor： Jiann-Hua Wang ,  Hui Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6417

Abstract: A fluorescent microarray analyzer includes a light source for emitting a light beam, a light processing unit for focusing the light beam on the biochip and exciting fluorescent targets on the biochip to produce fluorescence, a focusing lens for focusing the fluorescence on a spectrophotometer, a spectrophotometer for detecting signal of the fluorescence, and an output device for selectively outputting/displaying the signal detected by the spectrophotometer. The resulting signal of the output device does not need to be converted into image data for analysis. For acquiring a more accurate result of detection of signal of fluorescence from the fluorescent targets, the photomultiplier tube of the conventional biochip scanner device is replaced with the spectrophotometer of fluorescent microarray analyzer of the present invention and the filter is removed. Without converting the signal into an image, no errors arise as what happened in process of converting an electrical signal into image data in the conventional biochip. Also, a real-time analysis of the signal proceeds while scanning samples on the biochip.

Abstract translation: 荧光微阵列分析仪包括用于发射光束的光源，用于将光束聚焦在生物芯片上并激发生物芯片上的荧光靶产生荧光的光处理单元，用于将荧光聚焦在分光光度计上的聚焦透镜，分光光度计 用于检测荧光信号;以及输出装置，用于选择性地输出/显示由分光光度计检测的信号。 输出装置的结果信号不需要转换为图像数据进行分析。 为了从荧光靶获得更准确的荧光信号结果，用本发明的荧光微阵列分析仪的分光光度计代替常规生物芯片扫描器装置的光电倍增管，并除去滤光片。 在不将信号转换为图像的情况下，在传统生物芯片中将电信号转换为图像数据的过程中没有发生错误。 此外，在生物芯片上扫描样品时，信号的实时分析进行。

公开(公告)号：US20110165036A1

公开(公告)日：2011-07-07

申请号：US12651773

申请日：2010-01-04

Applicant: Tsung-Kai Chuang ,  Chien-Ho Chuang ,  Jiann-hua Wang

Inventor： Tsung-Kai Chuang ,  Chien-Ho Chuang ,  Jiann-hua Wang

IPC: G01N21/01 ,  G01N31/22 ,  G01N33/50 ,  G01N33/53

CPC classification number: G01N21/01 ,  G01N21/8483 ,  G01N2201/022

Abstract: An automatic physiological assay device analyzes light reflected by an assay device and prevents ambient light from interfering with the assay device. The automatic physiological assay device in accordance with the present invention comprises a chassis, an assay device connector assembly, an assay device and a light detecting device. The chassis comprises closed bottom, multiple sidewalls, an inner cavity, an open top and a removable top cover. The assay device connector assembly is mounted slidably through a sidewall and comprises an outer end, two sides and a cover. The cover is mounted on the outer end, seals the sidewall and keeps ambient light away from the assay device. The light detecting device is mounted in the inner cavity and on top edges of guides adjacent the assay device connector assembly.

Abstract translation: 自动生理测定装置分析由测定装置反射的光，并防止环境光干扰测定装置。 根据本发明的自动生理测定装置包括底盘，测定装置连接器组件，测定装置和光检测装置。 底盘包括封闭的底部，多个侧壁，内腔，敞开的顶部和可移除的顶盖。 测定装置连接器组件可滑动地安装在侧壁上并且包括外端，两侧和盖。 盖子安装在外端，密封侧壁并保持环境光远离测定装置。 光检测装置安装在内腔中并且在与测定装置连接器组件相邻的引导件的顶部边缘上。

公开(公告)号：US07173701B2

公开(公告)日：2007-02-06

申请号：US10777651

申请日：2004-02-13

Applicant: Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

Inventor： Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

IPC: G01J21/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6456

Abstract: A CCD-based biochip reader includes a light source for emitting light beams, a collimating lens for converting the light beams into wide parallel rays of light, passing said wide parallel rays of light through a biochip and exciting fluorescence from fluorescent targets on the biochip, a focusing lens for focusing the fluorescence, a filter for filtering out said parallel rays of light, and a charge-coupled device camera for generating images from said fluorescence. For recording intensity of the fluorescence from the fluorescent targets, images of the charge-coupled device camera is converted into digital data through an image converting device. Wide parallel laser beams are produced by the laser and through the collimating lens, and excite all samples on the biochip with high efficiency at the same time. In addition, time for analysis is saved when fluorescent images of a large area on biochips are collected and analyzed through the charge-coupled device camera.

Abstract translation: 基于CCD的生物芯片读取器包括用于发射光束的光源，用于将光束转换成宽平行光线的准直透镜，使所述宽平行光线通过生物芯片并从生物芯片上的荧光目标激发荧光， 用于聚焦荧光的聚焦透镜，用于滤出所述平行光线的滤光器和用于从所述荧光产生图像的电荷耦合器件相机。 为了记录来自荧光目标的荧光的强度，电荷耦合器件相机的图像通过图像转换装置转换成数字数据。 通过激光和准直透镜产生宽平行激光束，同时以高效率激发生物芯片上的所有样品。 此外，通过电荷耦合器件相机收集和分析生物芯片上大面积的荧光图像时，可节省分析时间。

公开(公告)号：US20060133959A1

公开(公告)日：2006-06-22

申请号：US11155584

申请日：2005-06-20

Applicant: Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

Inventor： Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

IPC: G01N21/64

CPC classification number: G01N21/253 ,  G01N21/76

Abstract: A luminometer includes a movable carrier carrying a number of luminescence collectors arranged in an array for simultaneously detecting the same number of samples received in multiple wells defined in a microplate. An optic fiber optically couples each luminescence detector to an associated charge-coupled device whereby a luminescence signal emitted from each sample is transmitted to the charge-coupled device and converted thereby into a corresponding electric signal. The charge-coupled devices are isolated from each other by partitions, which eliminate undesired interference among the luminescence signals transmitted to the charge-coupled device. A driving unit is provided to move the carrier between rows of the wells defined in the microplate.

Abstract translation: 发光计包括携带多个以阵列排列的发光收集器的可移动载体，用于同时检测在微板中限定的多个孔中接收的相同数量的样品。 光纤将每个发光检测器光耦合到相关联的电荷耦合器件，由此从每个样本发射的发光信号传输到电荷耦合器件，从而转换成相应的电信号。 电荷耦合器件通过分隔彼此隔离，这消除了传输到电荷耦合器件的发光信号之间的不期望的干扰。 提供了一个驱动单元，用于在微孔板中限定的孔的行之间移动载体。

公开(公告)号：US07042565B2

公开(公告)日：2006-05-09

申请号：US10777720

申请日：2004-02-13

Applicant: Jiann-Hua Wang ,  Hui Ju Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

Inventor： Jiann-Hua Wang ,  Hui Ju Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N2021/6417

Abstract: A fluorescent microarray analyzer includes a light source for emitting a light beam, a light processing unit for focusing the light beam on the biochip and exciting fluorescent targets on the biochip to produce fluorescence, a focusing lens for focusing the fluorescence on a spectrophotometer, a spectrophotometer for detecting signal of the fluorescence, and an output device for selectively outputting/displaying the signal detected by the spectrophotometer. The resulting signal of the output device does not need to be converted into image data for analysis. For acquiring a more accurate result of detection of signal of fluorescence from the fluorescent targets, the photomultiplier tube of the conventional biochip scanner device is replaced with the spectrophotometer of fluorescent microarray analyzer of the present invention and the filter is removed. Without converting the signal into an image, no errors arise as what happened in process of converting an electrical signal into image data in the conventional biochip. Also, a real-time analysis of the signal proceeds while scanning samples on the biochip.

Abstract translation: 荧光微阵列分析仪包括用于发射光束的光源，用于将光束聚焦在生物芯片上并激发生物芯片上的荧光靶产生荧光的光处理单元，用于将荧光聚焦在分光光度计上的聚焦透镜，分光光度计 用于检测荧光信号;以及输出装置，用于选择性地输出/显示由分光光度计检测的信号。 输出装置的结果信号不需要转换为图像数据进行分析。 为了从荧光靶获得更准确的荧光信号结果，用本发明的荧光微阵列分析仪的分光光度计代替常规生物芯片扫描器装置的光电倍增管，并除去滤光片。 在不将信号转换为图像的情况下，在传统生物芯片中将电信号转换为图像数据的过程中没有发生错误。 此外，在生物芯片上扫描样品时，信号的实时分析进行。