公开(公告)号：US20110035155A1

公开(公告)日：2011-02-10

申请号：US12745186

申请日：2008-11-13

Applicant: Michele Vendruscolo ,  Christopher Dobson ,  Gian Gaetano Tartaglia ,  Sebastian Pechmann

Inventor： Michele Vendruscolo ,  Christopher Dobson ,  Gian Gaetano Tartaglia ,  Sebastian Pechmann

IPC: G06F19/00 ,  G01N33/50

CPC classification number: G06F19/16 ,  G06F19/22 ,  G06F19/24

Abstract: We describe methods for identifying aggregation-prone regions in structured—that is folded—proteins. Embodiments of the method use a local propensity for aggregation (Ai) at an amino acid position, this being determined by a combination of a hydrophobicity value, an α-helix propensity value, a β-sheet propensity value, a charge value and a pattern value for the amino acid position. This is combined with local structural stability values for the amino acid positions to identify one or more regions in the amino acid sequence which, in the folded protein, are predicted to promote aggregation.

Abstract translation: 我们描述了在结构化中识别聚集多发区域的方法 - 折叠蛋白质。 该方法的实施方案在氨基酸位置使用局部聚集倾向（Ai），其通过疏水性值，α-螺旋倾向值，表面倾向度值，电荷值和 氨基酸位置的模式值。 这与氨基酸位置的局部结构稳定性值结合，以鉴定氨基酸序列中的一个或多个区域，折叠的蛋白质被预测为促进聚集。

公开(公告)号：US07698070B2

公开(公告)日：2010-04-13

申请号：US11417525

申请日：2006-05-04

Applicant: Christopher Dobson ,  Fabrizio Chiti ,  Jesus Zurdo ,  Kateri Hayashi DuBay ,  Michele Vendruscolo

Inventor： Christopher Dobson ,  Fabrizio Chiti ,  Jesus Zurdo ,  Kateri Hayashi DuBay ,  Michele Vendruscolo

IPC: G06F17/11 ,  G06F17/50 ,  G06F19/00

CPC classification number: G01N33/6818 ,  G06F19/16

Abstract: A method of determining aggregation rate data predicting an aggregation rate of a polypeptide defined by an amino acid sequence, the method comprising determining a hydrophobicity value, a charge value, and at least one shape propensity value for said sequence; identifying one or more aggregation-influencing patterns within said sequence; determining a pattern value for the sequence responsive to said identifying; and determining said aggregation rate data by determining a weighted combination of said hydrophobicity value, said charge value, said at least one shape propensity value, said pattern value and at least one factor extrinsic to said amino acid sequence.

Abstract translation: 一种确定聚集速率数据的方法，其预测由氨基酸序列定义的多肽的聚集速率，所述方法包括确定所述序列的疏水性值，电荷值和至少一种形状倾向度值; 识别所述序列内的一个或多个聚集影响模式; 响应于所述识别确定序列的模式值; 以及通过确定所述疏水性值，所述电荷值，所述至少一种形状倾向值，所述模式值和所述氨基酸序列的外在因素的至少一个因子的加权组合来确定所述聚集率数据。

公开(公告)号：US20090203535A1

公开(公告)日：2009-08-13

申请号：US12299408

申请日：2007-05-11

Applicant: Mark Welland ,  Christopher Dobson ,  Tuomas Knowles

Inventor： Mark Welland ,  Christopher Dobson ,  Tuomas Knowles

IPC: C40B30/04 ,  C40B60/14 ,  C40B50/18

CPC classification number: G01N33/6896 ,  G01N33/543 ,  G01N2333/4709 ,  G01N2500/00

Abstract: An acoustic wave transducer substrate is provided, the substrate having a surface with a plurality of growth promotion sites for promoting the accumulation of polypeptide molecules at said sites. The growth-promotion sites are preferably for promoting fibril formation. The invention also provides a method of screening a candidate compound for an effect on fibril growth, including the steps of: providing an acoustic wave transducer substrate having a surface with a plurality of growth promotion sites for promoting the accumulation of polypeptide molecules at said sites; causing oscillation of the substrate; contacting the substrate surface with a sample fluid comprising polypeptide molecules and a candidate compound; and measuring one or more parameters of the substrate oscillation to monitor the accumulation of said polypeptide molecules of interest in the presence and absence of said candidate compound. Alteration of the accumulation of said polypeptide molecules of interest in the presence of said candidate compound as compared with that in the absence of said candidate compound indicates that the candidate compound has an effect of fibril growth.

Abstract translation: 提供一种声波换能器基底，所述基底具有表面，所述表面具有多个生长促进位点，用于促进多肽分子在所述位点的积聚。 生长促进位点优选用于促进原纤维形成。 本发明还提供筛选候选化合物以对原纤维生长产生影响的方法，包括以下步骤：提供具有多个生长促进位点的表面的声波换能器底物，以促进多肽分子在所述位点的积聚; 引起基板的振荡; 使衬底表面与包含多肽分子和候选化合物的样品流体接触; 以及测量所述底物振荡的一个或多个参数以监测所述候选化合物存在和不存在的所述目的多肽分子的积累。 与不存在所述候选化合物相比，在所述候选化合物存在下，所述目的多肽分子的积累的改变表明候选化合物具有原纤维生长的作用。

公开(公告)号：US20070051768A1

公开(公告)日：2007-03-08

申请号：US11210982

申请日：2005-08-24

Applicant: Christopher Dobson ,  Luc Renaud

Inventor： Christopher Dobson ,  Luc Renaud

IPC: A45F3/00

CPC classification number: A45F5/02 ,  A45F5/021 ,  A63B47/04 ,  A63B55/20

Abstract: A golf ball carrier and cleaning device comprising a piece of fabric material, having opposite sides and a top and bottom, the top and bottom provided with spaced securing means positioned and constructed so as releasably to secure together the top and bottom, when the piece is folded about a central portion, and the securing means are in secured position, to form an open ended sleeve and provide an opening between the securing means and top and bottom through which a golf ball may be inserted into the sleeve, the piece being of a size and the securing means being positioned so that a golf ball inserted into the sleeve may be forced from the sleeve through one of its ends by squeezing the sleeve beside the ball, the piece further provided with means to suspend the piece from a desired location.

Abstract translation: 一种高尔夫球载体和清洁装置，其包括具有相对侧面和顶部和底部的一块织物材料，所述顶部和底部设置有间隔开的固定装置，所述固定装置被定位和构造成可释放地固定在顶部和底部的位置， 围绕中心部分折叠，并且固定装置处于固定位置，以形成开口的套筒，并且在固定装置和顶部和底部之间提供开口，高尔夫球可通过该顶部和底部插入套筒中， 尺寸和固定装置被定位成使得插入到套筒中的高尔夫球可以通过将套筒挤压在球旁边而从套筒中的一个端部被推压，该件还设置有将零件从期望位置悬挂下来的装​​置。

公开(公告)号：US20060271306A1

公开(公告)日：2006-11-30

申请号：US11417525

申请日：2006-05-04

Applicant: Christopher Dobson ,  Fabrizio Chiti ,  Jesus Zurdo ,  Kateri DuBay ,  Michele Vendruscolo

Inventor： Christopher Dobson ,  Fabrizio Chiti ,  Jesus Zurdo ,  Kateri DuBay ,  Michele Vendruscolo

IPC: G06F19/00

CPC classification number: G01N33/6818 ,  G06F19/16

Abstract: A method of determining aggregation rate data predicting an aggregation rate of a polypeptide defined by an amino acid sequence, the method comprising determining a hydrophobicity value, a charge value, and at least one shape propensity value for said sequence; identifying one or more aggregation-influencing patterns within said sequence; determining a pattern value for the sequence responsive to said identifying; and determining said aggregation rate data by determining a weighted combination of said hydrophobicity value, said charge value, said at least one shape propensity value, said pattern value and at least one factor extrinsic to said amino acid sequence.

Abstract translation: 一种确定聚集速率数据的方法，其预测由氨基酸序列定义的多肽的聚集速率，所述方法包括测定所述序列的疏水性值，电荷值和至少一种形状倾向度值; 识别所述序列内的一个或多个聚集影响模式; 响应于所述识别确定序列的模式值; 以及通过确定所述疏水性值，所述电荷值，所述至少一种形状倾向值，所述模式值和所述氨基酸序列的外在因素的至少一个因子的加权组合来确定所述聚集率数据。