公开(公告)号：US20210054421A1

公开(公告)日：2021-02-25

申请号：US17090569

申请日：2020-11-05

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： Eneko Ganuza Taberna ,  Magdalena Amezquita Ernult ,  Charles Sellers

IPC: C12P7/64 ,  C11B1/10 ,  C11C3/10 ,  C11B1/02

Abstract: Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.

公开(公告)号：US20200347418A1

公开(公告)日：2020-11-05

申请号：US16927568

申请日：2020-07-13

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Magdalena Amezquita Ernult ,  Charles Sellers

IPC: C12P7/64 ,  C11B1/10 ,  C11C3/10 ,  C11B1/02

Abstract: Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.

公开(公告)号：US20200267995A1

公开(公告)日：2020-08-27

申请号：US16333593

申请日：2017-06-16

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： Sandip SHINDE ,  Manakandadas Mathilakathu Madathil ,  Michael Warner ,  Stephen Ventre ,  Laura Carney ,  Michael Miller ,  Ganapathy Chellapan ,  Jon Hansen ,  Michael Lamont

IPC: A01N65/03 ,  C12P19/04

Abstract: The invention relates to compositions and methods for improving characteristics of plants and soil by administering an effective amount of a microalgae-based composition in low concentration applications.

公开(公告)号：US10745724B2

公开(公告)日：2020-08-18

申请号：US16229551

申请日：2018-12-21

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： Eneko Ganuza Taberna ,  Magdalena Amezquita Ernult ,  Charles Sellers

IPC: C12P7/64 ,  C11B1/10 ,  C11C3/10 ,  C11B1/02

Abstract: Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.

公开(公告)号：US20190177243A1

公开(公告)日：2019-06-13

申请号：US16131328

申请日：2018-09-14

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Suting Huang ,  Manikandadas Mathilakathu Madathil

IPC: C05F11/00 ,  C05G3/00 ,  A01G24/22 ,  A01C21/00

Abstract: A composition and method for making microalgae pre-charged biochar for use in soil is disclosed. The composition comprises raw biochar and a liquid microalgae composition, wherein the liquid microalgae composition comprises dead pasteurized Chlorella microalgae cells and nutrients that are beneficial to the soil; such as nitrogen, phosphorus, potassium, sulfur, and sodium. The raw biochar and liquid microalgae composition are combined to create a pre-charging mixture, which is then incubated for between 12-24 hours, and dried. The pre-charged biochar is then buried within the vicinity of a fruiting plant, seedling, or seed; between approximately 2-6 deep within the soil.

公开(公告)号：US10240120B2

公开(公告)日：2019-03-26

申请号：US14699659

申请日：2015-04-29

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Jason D Licamele ,  Anna Lee Tonkovich ,  Michael V Bellefeuille ,  Cameron Wyatt ,  Thomas J Kulaga ,  Adriano Galvez, III

IPC: C12N1/12 ,  C12Q3/00

Abstract: Methods of culturing mixotrophic microorganisms in a state of balanced gas composition are disclosed. Parameters of a culture of mixotrophic microorganisms may be controlled to reduce the requirements of externally supplied gases and optimize the production and consumption of gases within the culture by the phototrophic and heterotrophic metabolisms of the mixotrophic microorganisms.

公开(公告)号：US20190000091A1

公开(公告)日：2019-01-03

申请号：US16064094

申请日：2017-01-25

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Sandip Shinde ,  Laura Carney ,  Stephen Ventre

IPC: A01N65/03 ,  A01H5/10 ,  C12N1/12

Abstract: Compositions suitable for application to plants and seeds comprising dried Chlorella cells are disclosed. Methods of preparing and applying compositions of dried Chlorella cells to plants and seeds to enhance at least one characteristic of a plant are disclosed.

公开(公告)号：US09895403B2

公开(公告)日：2018-02-20

申请号：US15157047

申请日：2016-05-17

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Sandip Shinde ,  Stephen Ventre ,  Jennifer Lloyd-Randolfi ,  Mike Lamont

IPC: A61K36/02 ,  C12P7/64 ,  C11C3/00 ,  A61K8/37 ,  A61K31/231 ,  A61Q19/00

CPC classification number: A61K36/02 ,  A61K8/37 ,  A61K31/231 ,  A61Q19/00 ,  C11C3/003 ,  C12P7/6454

Abstract: Methods of producing high purity palmitoleic acid esters from natural oils are disclosed. The natural oils may comprise plant oil, nut oil, microalgae oil, and fish oil. The methods of processing the natural oil comprise transesterification with ethanol as the reacting solvent to produce ethyl esters. Methods of producing a high purity fraction of Omega-3, 6, & 9 fatty acid esters from natural oils are also disclosed. The high purity fatty acid esters may be used in nutrition, cosmetic, and nutraceutical products.

公开(公告)号：US20160281050A1

公开(公告)日：2016-09-29

申请号：US14795675

申请日：2015-07-09

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Laura Carney ,  Kristine Sorensen

IPC: C12N1/12 ,  C12Q1/06

CPC classification number: C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract translation: 本文描述了用过氧化氢和盐处理红潮球红藻的培养物中的污染物，特别是真菌污染物的方法。 该方法包括在培养过程中基于细胞阶段，并以增加细胞存活的可能性的频率，将包含一定浓度的过氧化氢浓度的培养物给药，直到累积类胡萝卜素如虾青素的过程 ， 做完了。

公开(公告)号：US09416347B2

公开(公告)日：2016-08-16

申请号：US14872448

申请日：2015-10-01

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Anna Lee Tonkovich

IPC: C12N1/12 ,  C12R1/89 ,  A61K36/02 ,  A01G33/00

CPC classification number: C12N1/12 ,  A01G33/00 ,  A61K36/02 ,  C12R1/89

Abstract: Methods of treating contamination, particularly bacterial contamination, in an open culture of microalgae with organic carbon are described herein. The methods comprise reducing the pH of a culture for a period of time and then raising the pH of the culture, wherein the culture comprises the presence of a first acid and the culture is contacted with a second acid to reduce the culture pH from a first pH value to a second pH value. The culture is maintained at the second pH value for at least 5 minutes before the culture pH is raised from the second pH value by contact with a base.