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    Low noise chemically-sensitive field effect transistors
    12.
    发明授权
    Low noise chemically-sensitive field effect transistors 有权
    低噪声化学敏感场效应晶体管

    公开(公告)号：US08445945B2

    公开(公告)日：2013-05-21

    申请号：US13554842

    申请日：2012-07-20

    Applicant: Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

    Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

    IPC: G01N27/403

    CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。

    Modified Proteins and Methods of Making and Using Same
    14.
    发明申请
    Modified Proteins and Methods of Making and Using Same 审中-公开
    改良蛋白质及其制备与使用方法

    公开(公告)号：US20120202276A1

    公开(公告)日：2012-08-09

    申请号：US13036623

    申请日：2011-02-28

    Applicant: John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

    Inventor： John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

    IPC: C12M1/40

    CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

    Modified Proteins and Methods of Making and Using Same
    15.
    发明申请
    Modified Proteins and Methods of Making and Using Same 审中-公开
    改良蛋白质及其制备与使用方法

    公开(公告)号：US20110318748A1

    公开(公告)日：2011-12-29

    申请号：US13035177

    申请日：2011-02-25

    Applicant: John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

    Inventor： John Davidson ,  Wolfgang Hinz ,  Jonathan Rothberg ,  Richard Whitaker

    IPC: C12Q1/68

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

    Particle Arrays and Methods of Making and Using
    16.
    发明申请
    Particle Arrays and Methods of Making and Using 审中-公开
    粒子阵列及其制作和使用方法

    公开(公告)号：US20110201523A1

    公开(公告)日：2011-08-18

    申请号：US13027336

    申请日：2011-02-15

    Applicant: Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

    Inventor： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

    IPC: C40B40/06

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中颗粒对颗粒的变化减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

    Methods of Using Porous Particles
    17.
    发明申请
    Methods of Using Porous Particles 审中-公开
    使用多孔颗粒的方法

    公开(公告)号：US20110201508A1

    公开(公告)日：2011-08-18

    申请号：US13029566

    申请日：2011-02-17

    Applicant: WOLFGANG HINZ ,  JOHN LEAMON ,  DAVID LIGHT ,  JONATHAN ROTHBERG

    Inventor： WOLFGANG HINZ ,  JOHN LEAMON ,  DAVID LIGHT ,  JONATHAN ROTHBERG

    IPC: C40B30/00 ,  C12Q1/68

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

    Scaffolded Nucleic Acid Polymer Particles and Methods of Making and Using
    18.
    发明申请
    Scaffolded Nucleic Acid Polymer Particles and Methods of Making and Using 审中-公开
    脚手架核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20110195252A1

    公开(公告)日：2011-08-11

    申请号：US13026707

    申请日：2011-02-14

    Applicant: Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

    Inventor： Wolfgang HINZ ,  John Leamon ,  David Light ,  Jonathan Rothberg

    IPC: B32B5/00 ,  C08F265/10

    CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

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