公开(公告)号：US10294484B2

公开(公告)日：2019-05-21

申请号：US14936840

申请日：2015-11-10

Applicant: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC ,  STELLENBOSCH UNIVERSITY

Inventor： Elena Brevnova ,  John E. McBride ,  Erin Wiswall ,  Kevin S. Wenger ,  Nicky Caiazza ,  Heidi Lau ,  Aaron Argyros ,  Frank Agbogbo ,  Charles F. Rice ,  Trisha Barrett ,  John S. Bardsley ,  Abigail Foster ,  Anne K. Warner ,  Mark Mellon ,  Ryan Skinner ,  Indraneel Shikhare ,  Riaan Den Haan ,  Chhayal V. Gandhi ,  Alan Belcher ,  Vineet B. Rajgarhia ,  Allan C. Froehlich ,  Kristen M. Deleault ,  Emily Stonehouse ,  Shital A. Tripathi ,  Jennifer Gosselin ,  Yin-Ying Chiu ,  Haowen Xu

IPC: C12N15/81 ,  C12N9/24 ,  C12N15/52 ,  C12N9/42 ,  C12P7/06

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

公开(公告)号：US10233414B2

公开(公告)日：2019-03-19

申请号：US15649075

申请日：2017-07-13

Applicant: Stellenbosch University

Inventor： Wessel Johannes Du Toit ,  Sebastian Vannevel

IPC: C12G1/02 ,  C12G1/04 ,  C12G3/02 ,  C12G3/08 ,  C12P11/00 ,  C12C5/00 ,  C12C5/02

Abstract: A method for preparing a fermented beverage having a modulated aromatic profile is provided as well as a fermented beverage produced thereby. The method includes preparing a fermentable mixture, such as juice, must, or wort and introducing ammonium sulphide into the fermentable mixture at a predetermined concentration. The fermentable mixture is then subjected to fermentation. A C6 aldehyde, C6 alcohol or a combination thereof may be added to the fermentable mixture in combination with ammonium sulphide to enhance its effect on the aromatic profile of the fermented beverage.

公开(公告)号：US10188689B2

公开(公告)日：2019-01-29

申请号：US15514248

申请日：2015-09-25

Applicant: STELLENBOSCH UNIVERSITY

Inventor： Nokwanda Pearl Makunga ,  Anna-Mart Engelbrecht

IPC: A61K36/77 ,  A61K9/00 ,  A61K39/00

Abstract: The invention disclosed herein relates to the use of an extract of Dodonaea viscosa in breast cancer therapy, either alone or in combination with other breast cancer therapies.

公开(公告)号：US10167459B2

公开(公告)日：2019-01-01

申请号：US15516372

申请日：2015-10-02

Applicant: Stellenbosch University

Inventor： Kim Trollope ,  Heinrich Volschenk ,  Johann Ferdinand Gorgens ,  Gerhardt Coetzee

IPC: C12N9/24 ,  C12N9/26 ,  A23L27/30 ,  A23L33/125 ,  C12P19/16

Abstract: The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesizing FOS using the variants is also claimed.

公开(公告)号：US10135156B2

公开(公告)日：2018-11-20

申请号：US15757253

申请日：2015-09-04

Applicant: STELLENBOSCH UNIVERSITY

Inventor： David Schalk Van der Merwe Prinsloo ,  Petrie Meyer ,  Rob Maaskant ,  Marianna Valerievna Ivashina

IPC: H01Q25/04 ,  H01Q3/24 ,  H01Q3/26 ,  H01Q9/28 ,  H01Q13/04 ,  H01Q13/08 ,  H01Q25/00 ,  H01Q5/48 ,  H01Q1/52 ,  H01Q21/26

Abstract: A multi-mode composite antenna includes two crossed dipole elements each consisting of a bow-tie antenna having two bow-tie antenna segments, and a conductive tube which houses signal transmission lines connected to each bow-tie antenna segment. A conductive flared portion surrounds the conductive tube and forms a monopole element. The bow-tie antenna segments are shaped so that slots extend between each adjacent bow-tie antenna, each slot forming a tapered slot antenna that has a pair of non-linear curved edges that diverge from each other.

公开(公告)号：US20180296196A1

公开(公告)日：2018-10-18

申请号：US15578842

申请日：2016-06-01

Applicant: NORTH-WEST UNIVERSITY ,  STELLENBOSCH UNIVERSITY ,  UNIVERSITY OF NEBRASKA-LINCOLN

Inventor： Anne Frederica GROBLER ,  Lizl CRONJE ,  Hendrik VILJOEN

IPC: A61B10/00 ,  A61J17/00 ,  G01N33/569

Abstract: This invention relates to a method and device for collecting a Mycobacterium complex species, such Mycobacterium tuberculosis (MTB) from the oral cavities of particularly children and infants. A device is provided which has a distal end and an operative end portion manufactured from a parent polymer which has been functionalised with a chemical compound having an affinity to the Mycobacterium complex species to collect same such that the presence thereof in the sample can be detected. The invention further extends to a method of preparing biological material obtained from such collected sample.

公开(公告)号：US20180155744A1

公开(公告)日：2018-06-07

申请号：US15830659

申请日：2017-12-04

Applicant: Stellenbosch University

Inventor： Rosemary Anne Cripwell ,  Willem Heber Van Zyl ,  Shaunita Hellouise Rose

IPC: C12P7/06 ,  C12N9/30 ,  C12N9/24

CPC classification number: C12P7/06 ,  C12N9/2405 ,  C12N9/242 ,  C12P7/16 ,  C12Y302/01 ,  C12Y302/01001 ,  Y02E50/10 ,  Y02E50/17

Abstract: A recombinant yeast that expresses both an α-amylase (SEQ ID NO: 1) and a glucoamylase (SEQ ID NO: 2) from Talaromyces emersonii (recently re-named as Rasamsonia emersonii) is provided. The use of the recombinant yeast in a process for producing an alcohol, in particular a biofuel, from starch or sugars is also described.

公开(公告)号：US09963209B2

公开(公告)日：2018-05-08

申请号：US14647646

申请日：2013-11-27

Applicant: Stellenbosch University

Inventor： Craig Patrick O'Connell ,  Michael Rutzen ,  Sara Andreotti ,  Conrad Matthee

IPC: A01K61/00 ,  B63C9/05 ,  A01K79/02 ,  A01K61/70 ,  E02B3/04

CPC classification number: B63C9/05 ,  A01K61/70 ,  A01K79/02 ,  E02B3/043 ,  Y02A40/83

Abstract: A shark barrier includes a plurality of resiliently flexible, elongate members that extend in a generally upright condition between a sea floor and a sea surface. The elongate members are arranged so as to have the appearance of a thicket, preferably a kelp forest, when viewed from within the water and include magnets to assist in deterring certain shark species. The elongate members may be secured to the sea floor by an anchoring base and are held in the generally upright condition by a buoy or buoyant material held within the elongate members.

公开(公告)号：US20170298332A1

公开(公告)日：2017-10-19

申请号：US15516372

申请日：2015-10-02

Applicant: Stellenbosch University

Inventor： Kim Trollope ,  Heinrich Volschenk ,  Johann Ferdinand Gorgens ,  Gerhardt Coetzee

IPC: C12N9/26 ,  A23L27/33 ,  A23L33/125 ,  C12P19/16

CPC classification number: C12N9/2431 ,  A23L27/33 ,  A23L33/125 ,  A23V2002/00 ,  C12P19/16 ,  C12Y302/01026

Abstract: The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesising FOS using the variants is also claimed.

公开(公告)号：US20170232110A1

公开(公告)日：2017-08-17

申请号：US15503175

申请日：2015-08-12

Applicant: Stellenbosch University

Inventor： Lubertus Klumperman ,  Paul William Reader ,  Marina Rautenbach

IPC: A61K47/48 ,  A61K38/08 ,  C08F226/10 ,  C08G63/48

CPC classification number: C08F226/10 ,  A61K38/08 ,  A61K47/58 ,  A61K47/6907 ,  C08F2810/40 ,  Y02A50/411

Abstract: A peptide-polymer conjugate is provided for use in treating malaria infections, and in particular terminal or drug resistant malaria infections. The conjugate is formed from a polymer to which a peptide having activity against a malaria parasite is co-valently attached. The peptide is a cyclic decapeptide from the closely-related group of tyrocidines, tryptocidines, phenycidines and gramicidin S, and the polymer is a hydrophilic and biocompatible polymer with a terminal thiol, such as poly(N-vinylpyrrolidone) (PVP). The polymer chains can be decorated with a hydrophilic targeting ligand that specifically targets an epitope on red blood cells, and in particular red blood cells infected with a plasmodial parasite. A method for synthesising the peptide-polymer conjugate is also provided.