公开(公告)号：US12168231B2

公开(公告)日：2024-12-17

申请号：US17486804

申请日：2021-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Billy W. Colston, Jr. ,  Benjamin J. Hindson ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  B01L3/02 ,  B01L7/00 ,  B29C45/00 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/64 ,  B01F101/23 ,  B29L31/00

Abstract: Method of analysis. In the method, a microfluidic device defining a flow path extending from an inlet to an outlet may be selected. A sample-containing fluid may be introduced into the flow path via the inlet. Volumes of the sample-containing fluid may be isolated from one another on the flow path. A two-dimensional monolayer of the volumes may be imaged. The two-dimensional monolayer may be formed along the flow path between the inlet and the outlet.

公开(公告)号：US10099219B2

公开(公告)日：2018-10-16

申请号：US15357840

申请日：2016-11-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  G01N35/08 ,  C12Q1/6806 ,  B01L3/02 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20180147573A1

公开(公告)日：2018-05-31

申请号：US15707908

申请日：2017-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, Jr. ,  Erin R. Chia ,  Billy W. Colston, Jr. ,  Serge Saxonov ,  Svilen S. Tzonev ,  Michael Y. Lucero ,  Ryan T. Koehler

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  C12Q1/686 ,  B01F13/10 ,  B01F3/08 ,  B01F5/02 ,  B01F5/00 ,  G01N35/08

CPC classification number: B01L3/50273 ,  B01F3/0807 ,  B01F5/0085 ,  B01F5/0256 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/1022 ,  B01L3/502 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  C12Q1/686 ,  G01N35/08

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

公开(公告)号：US09901927B2

公开(公告)日：2018-02-27

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, Jr. ,  Fred P. Milanovich ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  C12Q1/68 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US20170144161A1

公开(公告)日：2017-05-25

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, JR. ,  Fred P. Milanovich ,  Donald A. Masquelier ,  Anthony J. Makarewicz, JR.

IPC: B01L3/00 ,  B01F13/00 ,  G01N21/64 ,  B29C45/00 ,  C12Q1/68 ,  B01L7/00 ,  B01F3/08 ,  B01F15/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US09417190B2

公开(公告)日：2016-08-16

申请号：US13945661

申请日：2013-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6848 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6441 ,  G01N2201/1242 ,  C12Q2545/101 ,  C12Q2563/107 ,  C12Q2563/159

Abstract: System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.

Abstract translation: 用于执行使用从液滴检测的信号来控制和/或校准的基于液滴的测定的系统，包括方法和装置。

公开(公告)号：US20160059204A1

公开(公告)日：2016-03-03

申请号：US14938470

申请日：2015-11-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01J19/00 ,  G01N1/44 ,  G01N1/30

CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F13/0079 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00873 ,  B01J2219/00894 ,  B01J2219/00903 ,  B01J2219/00957 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0677 ,  C12Q1/6844 ,  C40B50/08 ,  C40B60/14 ,  G01N1/30 ,  G01N1/44 ,  G01N21/75 ,  G01N33/50 ,  G01N35/08 ,  C12Q2547/107

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

Abstract translation: 系统，包括方法，装置，组合物和试剂盒，用于通过多个乳液的聚结来混合小体积的流体。

公开(公告)号：US12103005B2

公开(公告)日：2024-10-01

申请号：US16995555

申请日：2020-08-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Christopher F. Kelly ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/02 ,  B01L7/00 ,  C12Q1/6806 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502784 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/502715 ,  B01L3/502723 ,  C12Q1/6806 ,  G01N35/085 ,  B01L3/0275 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N2035/00148 ,  G01N2035/1034 ,  G01N35/1065 ,  Y10T29/494

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US11633739B2

公开(公告)日：2023-04-25

申请号：US17137274

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  C12Q1/686 ,  G01N21/64 ,  B29C45/00 ,  G01N21/49 ,  B01F101/23 ,  B29L31/00

Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

公开(公告)号：US20220008914A1

公开(公告)日：2022-01-13

申请号：US17486667

申请日：2021-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, JR. ,  Erin R. Chia ,  Billy W. Colston, JR. ,  Serge Saxonov ,  Svilen S. Tzonev ,  Michael Y. Lucero ,  Ryan T. Koehler

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01F13/10 ,  B01L9/00 ,  C12Q1/686

Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.