公开(公告)号：US10436697B2

公开(公告)日：2019-10-08

申请号：US15817237

申请日：2017-11-19

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： David Vrane

IPC: G01N15/10 ,  G01N15/14 ,  G01N21/49

Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.

公开(公告)号：US20180024040A1

公开(公告)日：2018-01-25

申请号：US15659610

申请日：2017-07-25

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： Ming Yan ,  Yung-Chieh Hsieh ,  David Vrane ,  Eric Chase

IPC: G01N15/14 ,  G01J1/08 ,  G01N21/47

CPC classification number: G01N15/1436 ,  G01J1/08 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0256 ,  G01J3/0291 ,  G01J3/36 ,  G01J2003/1213 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/47 ,  G01N2015/1006 ,  G01N2015/145

Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

公开(公告)号：US12228494B2

公开(公告)日：2025-02-18

申请号：US18628729

申请日：2024-04-07

Applicant: Cytek Biosciences, Inc.

Inventor： David Vrane

IPC: G01N15/14 ,  G01N15/1404 ,  G01N15/10 ,  G01N15/1409

Abstract: A pressure regulated fluidics system of a flow cytometer includes a sheath tank holding sheath fluid; a degasser coupled to the sheath tank by a first sheath line; a manifold assembly coupled to the degasser by a second sheath line; a first valve coupled to the second sheath line; a pressure regulator coupled to the sheath tank; and a transducer coupled to and between the pressure regulator and the first valve. The transducer senses measured pressure and converts it into a voltage. The pressure regulator applies regulated pressure to the sheath fluid to maintain a total flow rate of fluids through the flow cytometer based on the voltage. The degasser pulls gas molecules out of the sheath fluid.

公开(公告)号：US12162013B2

公开(公告)日：2024-12-10

申请号：US17829321

申请日：2022-05-31

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： Glen Krueger ,  David Vrane ,  Kuncheng Wang ,  Qiuta Gu

IPC: B01L3/00 ,  C12M1/00 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1409 ,  G01N15/1434 ,  G01N15/149

Abstract: A compact sorting flow cytometer system is disclosed. The system includes a flow cell and a deflection unit in communication with the flow cell to receive charged drops and uncharged drops in a stream of a sample biological fluid. The deflection unit includes a case having a deflection cone and chamber, a tub with a drain, and a slot in a base. The deflection unit further includes electrostatic charge plates in the cone forming an electrostatic charge field; and first and second pivotal scuppers, and a center non-pivotal collector arranged along a shaft in the tub of the case. Uncharged drops pass the electrostatic charge field undeflected in a center stream path while charged drops are deflected away from it. The pivotal scuppers pivot between positions to direct charged drops into the tub for aspiration or to pass through the slot in the base for collection.

公开(公告)号：US20240280464A1

公开(公告)日：2024-08-22

申请号：US18628729

申请日：2024-04-07

Applicant: Cytek Biosciences, Inc.

Inventor： David Vrane

IPC: G01N15/1404 ,  G01N15/10 ,  G01N15/1409

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N15/1409

Abstract: A pressure regulated fluidics system of a flow cytometer includes a sheath tank holding sheath fluid; a degasser coupled to the sheath tank by a first sheath line; a manifold assembly coupled to the degasser by a second sheath line; a first valve coupled to the second sheath line; a pressure regulator coupled to the sheath tank; and a transducer coupled to and between the pressure regulator and the first valve. The transducer senses measured pressure and converts it into a voltage. The pressure regulator applies regulated pressure to the sheath fluid to maintain a total flow rate of fluids through the flow cytometer based on the voltage. The degasser pulls gas molecules out of the sheath fluid.

公开(公告)号：US20230405592A1

公开(公告)日：2023-12-21

申请号：US18297615

申请日：2023-04-08

Applicant: Cytek Biosciences, Inc.

Inventor： Kuncheng Wang ,  Huimin Gu ,  Andrew Klyce-Mariscal ,  Wing Lee ,  Ming Yan ,  Glen Krueger ,  David Vrane

IPC: B01L3/00 ,  G01N15/14

CPC classification number: B01L3/502746 ,  G01N15/1434 ,  G01N2015/149 ,  G01N2015/1006

Abstract: A flow cell subassembly includes a carriage assembly coupled to a flow cell body to selectively engage a nozzle with a base of a cuvette. The carriage assembly includes a linear bearing slidingly engaged with the flow cell body, a tiltable carriage plate coupled to the linear bearing, and a nozzle mount coupled to the tiltable carriage plate. The nozzle mount receives a nozzle assembly with the nozzle. Set screws can adjust pitch angle of the tiltable carriage plate with the linear bearing to adjust engagement between the nozzle and cuvette in a first dimension. To adjust engagement between the nozzle and cuvette in a second dimension, axial play in bolts/screws through the tiltable carriage plate into threaded holes of the linear bearing allow for yaw angle adjustments.

公开(公告)号：US20230288313A1

公开(公告)日：2023-09-14

申请号：US18319048

申请日：2023-05-17

Applicant: Cytek Biosciences, Inc.

Inventor： Ming Yan ,  Yung-Chieh Hsieh ,  David Vrane ,  Eric Chase

IPC: G01N15/14 ,  G01J1/08 ,  G01N21/47 ,  G01J3/02 ,  G01J3/36

CPC classification number: G01N15/1436 ,  G01J1/08 ,  G01N21/47 ,  G01N15/1459 ,  G01N15/1434 ,  G01J3/0218 ,  G01J3/0291 ,  G01J3/0256 ,  G01J3/36 ,  G01J3/021 ,  G01J3/0208 ,  G01N2015/145 ,  G01N2015/1006

Abstract: A method includes launching, from an optical fiber, fluorescent light of differing wavelengths generated by different fluorochromes attached to particles in a sample fluid; magnifying an image size from an end of the optical fiber to a first dichroic filter of a row of a plurality of dichroic filters in a de-multiplexing imaging array; alternatively reflecting the fluorescent light between the plurality of dichroic filters and a plurality of micro-mirrors to collimate the fluorescent light on odd numbered dichroic filters and re-image the fluorescent light on even numbered dichroic filters; band passing different wavelength ranges of the fluorescent light at each of the plurality of dichroic filters to de-multiplex the wavelength spectrum of the wavelength range of the fluorescent light; and detecting fluorescent light in each of the different wavelength ranges to count a number of each of the different particles in the sample fluid.

公开(公告)号：US12140524B2

公开(公告)日：2024-11-12

申请号：US17120120

申请日：2020-12-12

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： David Vrane

IPC: G01N15/1404 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1409 ,  G01N15/1434

Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell. A stepper flow control valves are disclosed that apply a physical fluid resistance to a flow of sheath fluid in the flow cytometer. The physical fluid resistance regulates a flow rate of the sheath fluid and thereby regulates a flow rate of sample fluid in the flow cytometer.

公开(公告)号：US20240159648A1

公开(公告)日：2024-05-16

申请号：US18420186

申请日：2024-01-23

Applicant: Cytek Biosciences, Inc.

Inventor： Janine Jiang ,  David Vrane

IPC: G01N15/1429 ,  G01N15/14 ,  G01N15/1434

CPC classification number: G01N15/1431 ,  G01N15/1425 ,  G01N15/1434 ,  G01N2015/1438

Abstract: A smart flow cytometer is disclosed including a plurality of tanks holding fluids for operation of a flow cytometer; a plurality of level sensors respectively coupled to the plurality of tanks; a plurality of valves coupled to one or more tubes for operation of the flow cytometer; one or more flow sensors coupled to the one or more tubes for monitoring flow rates; and a microcontroller coupled to the plurality of level sensors and the one or more flow sensors. The microcontroller constantly monitors fluid levels of the plurality of tanks to detect when to refill a fluid in a first tank before the first tank is completely empty and to detect when to empty a second tank before the second tank becomes completely filled with a waste fluid.

公开(公告)号：US20240027321A1

公开(公告)日：2024-01-25

申请号：US18221349

申请日：2023-07-12

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： David Vrane

IPC: G01N15/14 ,  G01N21/49

CPC classification number: G01N15/1404 ,  G01N15/1436 ,  G01N21/49 ,  G01N15/1434 ,  G01N15/147 ,  G01N2015/1075

Abstract: A system, method, and apparatus are provided for flow cytometry. In one example, the flow cytometry system includes dual laser devices and dual scatter channels to measure velocity of particles in a core stream of sample fluid. The total flow rate of the sample fluid and the sheath fluid around the sample fluid is controlled, and thus held constant, by a feedback control system controlling a vacuum pump based on differential pressure across ends of a flow channel in the flow cell.