公开(公告)号：US07948184B2

公开(公告)日：2011-05-24

申请号：US11906573

申请日：2007-10-02

Applicant: David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

Inventor： David M. Heffelfinger ,  David A. Pringle ,  David B. McDonald ,  Ashot Nalbandyan

IPC: B60Q1/14

CPC classification number: G03B15/05 ,  G03B2215/05

Abstract: A vehicle testing lamp system comprising an onboard controller unit, an off-board controller unit, and a lamp fixture. The onboard controller unit comprises a solid state relay, the solid state relay being switchable between an idle output power setting and a high output power setting; a timing circuit,; a transfer circuit; a current sensor; a current regulator; and batteries or other auxiliary power supply. The off-board controller unit comprises a power supply electrically connected to a voltage booster circuit. The lamp fixture comprises an ignitor; a shock mount; and a lamp that is electrically connected to the igniter. The off-board controller supplies power to the lamp at its idle level. The onboard transfer circuit permits the off-board power supply to be disconnected while the onboard controller unit maintains the lamp at idle, and the onboard timing circuit limits the time that the lamp remains at high output. Also disclosed is a method for using a vehicle testing lamp system.

Abstract translation: 一种车辆测试灯系统，包括车载控制器单元，车外控制器单元和灯具。 车载控制器单元包括固态继电器，固态继电器可在空转输出功率设定和高输出功率设定之间切换; 定时电路 传输电路; 电流传感器; 现行监管机构 电池或其他辅助电源。 车外控制器单元包括电连接到升压电路的电源。 灯具包括点火器; 一个防震架; 以及电连接到点火器的灯。 车外控制器在其空闲水平下为灯供电。 车载传输电路允许车载电源断开，而车载控制器单元将灯保持在空闲状态，并且车载定时电路限制灯保持在高输出的时间。 还公开了一种使用车辆测试灯系统的方法。

公开(公告)号：US5897760A

公开(公告)日：1999-04-27

申请号：US814127

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26

CPC classification number: G01N27/44721

Abstract: A method and apparatus for correcting non-uniformities in the source and the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity, correcting for source and lens non-uniformities allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. The non-uniformities due to the illumination source are characterized by sampling a portion of the source with a linear detector array and creating a correction data file. In order to sample the source, a mirror or beamsplitter is appropriately positioned, for example along the central optical axis of the electrophoresis apparatus. Similarly, a correction data file representing the non-uniformities due to the lens assembly is created using a secondary linear source of known uniformity. After the correction data files are stored, an image of the sample is taken and a sample data file is created. The sample data file can be normalized using the correction data files thereby creating a corrected sample file which can either be displayed or stored for future use.

Abstract translation: 提供了一种用于校正电泳装置的源和透镜组件中的不均匀性的方法和装置。 假设具有均匀响应度的检测器，校正源和透镜不均匀性允许对电泳凝胶进行定量测量，从而增加可从电泳分析获得的信息。 由于照明源引起的不均匀性的特征在于用线性检测器阵列对源的一部分进行采样并创建校正数据文件。 为了对源进行采样，镜子或分束器例如沿着电泳装置的中心光轴被适当地定位。 类似地，使用已知均匀性的二次线性源产生表示由于透镜组件引起的不均匀性的校正数据文件。 在存储校正数据文件之后，取样本的图像并创建样本数据文件。 样本数据文件可以使用校正数据文件进行归一化，从而创建可以显示或存储以供将来使用的校正样本文件。

公开(公告)号：US5792610A

公开(公告)日：1998-08-11

申请号：US640658

申请日：1996-05-01

Applicant: Franklin R. Witney ,  David M. Heffelfinger

Inventor： Franklin R. Witney ,  David M. Heffelfinger

IPC: C12Q1/68 ,  G01B9/02 ,  G01B11/00 ,  G01T3/45

CPC classification number: C12Q1/6841 ,  C12Q1/6816

Abstract: The invention relates to a method of identifying the individual autosomal and sex chromosomes of a human karyotype through the use of a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said set, and (ii) being capable of specifically hybridizing with one predetermined autosomal or sex chromosome of a human karyotype.

Abstract translation: 本发明涉及通过使用一组组合标记的寡核苷酸探针来鉴定人类核型的个体常染色体和性染色体的方法，每一个成员：（i）具有与所述的任何其他成员的标签可区分的预定标签 设置，和（ii）能够与人核型的一个预定的常染色体或性染色体特异性杂交。

公开(公告)号：US07016087B2

公开(公告)日：2006-03-21

申请号：US09925124

申请日：2001-08-08

Applicant: David M. Heffelfinger ,  Aram P. Schiffman ,  Bala S. Manian

Inventor： David M. Heffelfinger ,  Aram P. Schiffman ,  Bala S. Manian

IPC: H04N1/04

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A system for optical interrogation of a sample adaptable for multiple wavelength illumination and multiple wavelength fluorescent or luminescent light collection, wherein the illumination wavelength profile and the light collection profile may overlap. In the system, coherent light from one or more lasers is focused onto a target layer on a sample to excite fluorescent or luminescent light from the target layer. Emitted light is collected from a selected depth by a reflective light collector that transmits the collected light to detection optics. The reflective light collector directs collected light at an angle to the optical axis of the illumination light, thereby separating collected emitted light from illumination light. The light collector may collect light from a focus, whereby the focused illumination light combined with the focused light collection aid in limitation of the depth of field to a selected depth. Additionally, a spatial filter positioned between the light collector and the detection optics may be used to confine the depth of field to a selected depth. This device may be incorporated into an optical scanner by scanning of illumination light in a first direction and translation of the sample in a tangent direction. Alternatively, the illumination and detection optics may remain stationary and the detectable targets moved past a scanning location (e.g. as in electrophoretic analysis).

公开(公告)号：US06995901B2

公开(公告)日：2006-02-07

申请号：US10758140

申请日：2004-01-15

Applicant: David M. Heffelfinger

Inventor： David M. Heffelfinger

IPC: G02B21/00

CPC classification number: G02B21/16

Abstract: A system for the optical analysis of a sample. An illumination source illuminates the sample, exciting fluorescence. The fluorescence is collected by an objective lens, which transmits the collected illumination light onto an imaging lens, which focuses the collected light onto an area array detector. Collected light rays between the objective lens and the imaging lens are parallel and pass through an emission filter. Both the objective lens and the imaging lens are positioned on a mount that allows an alternative objective or imaging lens to be positioned to collect or image the emitted light. Any objective lens/imaging lens pair is optically symmetrical, greatly reducing the optically degrading effects.

Abstract translation: 用于样品光学分析的系统。 照明源照亮样品，激发荧光。 荧光被物镜收集，物镜将收集的照明光透射到成像透镜上，该成像透镜将收集的光聚焦到区域阵列检测器上。 物镜和成像透镜之间的收集的光线平行并通过发射滤光片。 物镜和成像透镜都位于允许替代物镜或成像透镜被定位以收集或成像发射光的安装座上。 任何物镜/成像透镜对都是光学对称的，大大降低了光学降解效果。

公开(公告)号：US6043506A

公开(公告)日：2000-03-28

申请号：US1254

申请日：1997-12-30

Applicant: David M. Heffelfinger ,  Rebecca Ann Batterson ,  Renato Salgado

Inventor： David M. Heffelfinger ,  Rebecca Ann Batterson ,  Renato Salgado

IPC: G01N21/64 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/75 ,  G01N21/78 ,  G01N33/53 ,  G01N37/00 ,  G03B42/00 ,  G03B42/02

CPC classification number: G03B42/00 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6471 ,  G01N2021/6484 ,  G01N2201/024

Abstract: An apparatus capable of measuring quantities of biological or other types of samples that have been labeled using any of a variety of techniques including fluorescence, radioisotopes, enzyme activated light emitting chemicals, and enzyme activated fluorescent materials is provided. The apparatus allows for either simultaneous or sequential acquisition of signals from multiple sample types. The apparatus is not restricted to a particular source or wavelength of excitation or readout light, nor is the apparatus restricted to a particular emission wavelength. The provided scanner includes a source module that preferably contains an internal laser emitting two different wavelengths of approximately the same intensity. An optional external light source may be coupled to the source module, thus adding further flexibility through the addition of other wavelengths (e.g., V, visible, mid-IR, and IR). The scanner also includes a detection module. Within the detection module are two detectors, thus allowing the simultaneous detection of multiple wavelengths. A bifurcated optical cable is used to transfer the excitation and/or readout light from the source module to the sample and subsequently transfer the emitted and/or scattered light from the sample to the detection module. The scanning stage of the scanner is designed to accommodate a variety of samples, ranging from phosphor screens, gels, and fluorescent samples to microtiter plates. An internal microprocessor is used to control the various aspects of the scanner, preferably including translation stage control, source filters, and detection filters. The internal microprocessor may be coupled to an external computer. The external computer may be used to change the programming of the microprocessor, provide a user interface to the microprocessor, process and store test results, and display sample images.

Abstract translation: 提供了一种能够测量使用包括荧光，放射性同位素，酶活化的发光化学物质和酶激活的荧光材料在内的各种技术中的任何一种进行标记的生物样品或其他类型样品的量的装置。 该装置允许同时或顺序地采集来自多个样本类型的信号。 该装置不限于激发或读出光的特定源或波长，也不限于特定发射波长的装置。 所提供的扫描器包括源模块，其优选地包含发射具有大致相同强度的两个不同波长的内部激光器。 可选的外部光源可以耦合到源模块，从而通过添加其它波长（例如，V，可见，中IR和IR）来增加更多的灵活性。 扫描仪还包括检测模块。 在检测模块内是两个检测器，从而允许同时检测多个波长。 使用分叉光缆将激励和/或读出光从源模块传送到样品，随后将发射和/或散射的光从样品转移到检测模块。 扫描仪的扫描阶段设计为适应各种样品，从荧光屏，凝胶和荧光样品到微量滴定板。 内部微处理器用于控制扫描仪的各个方面，最好包括平移台控制，源滤波器和检测滤波器。 内部微处理器可以耦合到外部计算机。 外部计算机可用于改变微处理器的编程，向微处理器提供用户界面，处理和存储测试结果以及显示样本图像。

公开(公告)号：US5891314A

公开(公告)日：1999-04-06

申请号：US814812

申请日：1997-03-10

Applicant: David M. Heffelfinger ,  Craig Van Horn

Inventor： David M. Heffelfinger ,  Craig Van Horn

IPC: G01N27/447 ,  G01N27/26 ,  G01N21/00

CPC classification number: G01N27/44721

Abstract: A method and apparatus for correcting non-uniformities in the lens assembly of an electrophoresis apparatus is provided. Assuming a detector with a uniform responsivity as well as a uniform illumination source, correcting for lens non-uniformities allows accurate quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Applying the system, the non-uniformities due to the lens assembly are first characterized for a range of aperture and magnification settings. A look-up table is then created which contains the non-uniformities and/or correction data files for the lens assembly according to the aperture and magnification settings. In order to correct a sample image, the aperture and magnification settings used to obtain the sample image are provided to the system processor. These settings may be automatically obtained by the processor or manually input by the user. After the processor receives the lens settings, it applies the look-up table to determine the corresponding lens non-uniformities as well as the necessary correction file. The sample image is then normalized by dividing the sample image file by the appropriate correction file. Once normalized, the corrected sample image file may either be displayed or stored for later use.

Abstract translation: 提供了一种用于校正电泳装置的透镜组件中的不均匀性的方法和装置。 假设具有均匀响应性的检测器以及均匀的照明源，校正透镜不均匀性可以准确地定量测量电泳凝胶，从而增加可从电泳分析获得的信息。 应用该系统，由于镜头组件而引起的不均匀性首先被表征为一定范围的孔径和放大倍率设置。 然后创建一个查找表，其中包含根据孔径和放大倍率设置的镜头组件的不均匀性和/或校正数据文件。 为了校正样本图像，将用于获得样本图像的孔径和倍率设置提供给系统处理器。 这些设置可以由处理器自动获取或由用户手动输入。 在处理器接收到镜头设置后，应用查找表来确定相应的镜头不均匀性以及必要的校正文件。 然后通过将样本图像文件除以适当的校正文件来对样本图像进行归一化。 一旦标准化，校正的样本图像文件可以被显示或存储以供以后使用。

公开(公告)号：US5885531A

公开(公告)日：1999-03-23

申请号：US927556

申请日：1997-09-09

Applicant: David M. Heffelfinger ,  Franklin R. Witney

Inventor： David M. Heffelfinger ,  Franklin R. Witney

IPC: G01N21/91 ,  G01N21/25 ,  G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10S435/81

Abstract: A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.

Abstract translation: 提供了成像荧光原位杂交（FISH）的方法和装置。 该仪器允许用户同时采集几种不同颜色的图像。 该系统与组合荧光方法结合使用，能够产生FISH染色体核型，每个染色体都涂上不同的颜色。 光学系统在检测波长上连续可调。 在系统的一个实施例中，样本同时在多于一个波长带中照射，并且检测系统使用公共路径干涉仪来扫描检测波长。

公开(公告)号：US5863504A

公开(公告)日：1999-01-26

申请号：US585303

申请日：1996-01-11

Applicant: David M. Heffelfinger ,  Franklin R. Witney

Inventor： David M. Heffelfinger ,  Franklin R. Witney

IPC: G01N21/91 ,  G01N21/25 ,  G01N21/64 ,  C12M1/40 ,  C12Q1/68

CPC classification number: G01N21/6458 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  Y10S435/81

Abstract: A method and apparatus of imaging fluorescence in situ hybridization (FISH) is provided. The instrument allows the user to simultaneously acquire images from several different colors. This system, used in conjunction with a combinatorial fluorescence approach, is able to create a FISH karyotype with each chromosome being painted with a different color. The optical system is continuously tunable over the detection wavelengths. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band and the detection system uses a common path interferometer to scan through the detection wavelengths.