公开(公告)号：US5105007A

公开(公告)日：1992-04-14

申请号：US721742

申请日：1991-06-26

Applicant: Maciej B. Adamczyk ,  Hossein A. Ghanbari ,  Donald D. Johnson

Inventor： Maciej B. Adamczyk ,  Hossein A. Ghanbari ,  Donald D. Johnson

IPC: G01N33/53 ,  G01N33/531 ,  G01N33/58 ,  G01N33/68

CPC classification number: G01N33/582 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/6812

Abstract: The present invention is directed to a fluorescence polarization immunoassay for determining the phenylacetylglutamine (PAG) content in body fluids, to the various components needed for preparing and carrying out such an assay, and to the methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them. The assay is conducted by measuring the degree of polarization of plane polarized light that has been passed through a solution continuing sample, antiserum and tracer.

Abstract translation: 本发明涉及用于测定体液中苯乙酰谷氨酰胺（PAG）含量，制备和进行这种测定所需的各种组分的荧光偏振免疫测定，以及制备这些组分的方法。 具体地，公开了示踪剂，免疫原和抗体，以及其制备方法。 该测定通过测量已经通过溶液持续样品，抗血清和示踪剂的平面偏振光的偏振度来进行。

公开(公告)号：US5101015A

公开(公告)日：1992-03-31

申请号：US335627

申请日：1989-04-10

Applicant: Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

Inventor： Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

IPC: G01N33/53 ,  A61K39/00 ,  G01N33/531 ,  G01N33/533 ,  G01N33/542 ,  G01N33/94

CPC classification number: G01N33/542 ,  G01N33/533 ,  G01N33/946 ,  Y10S436/816 ,  Y10S436/901 ,  Y10T436/13

Abstract: A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

公开(公告)号：US20130034854A1

公开(公告)日：2013-02-07

申请号：US13640944

申请日：2011-04-27

Applicant: Julia Ashworth-Sharpe ,  Chol Steven Yun ,  Zhanna Zhilina ,  Adrian E. Murillo ,  Donald D. Johnson ,  Michael Farrell ,  Jerome W. Kosmeder ,  Christopher Bieniarz

Inventor： Julia Ashworth-Sharpe ,  Chol Steven Yun ,  Zhanna Zhilina ,  Adrian E. Murillo ,  Donald D. Johnson ,  Michael Farrell ,  Jerome W. Kosmeder ,  Christopher Bieniarz

IPC: C07K17/14 ,  G01N33/566 ,  G01N33/573 ,  G01N33/553 ,  C12Q1/68 ,  B82Y5/00

CPC classification number: G01N33/54346 ,  C07F9/12 ,  C07F9/5004 ,  C07F9/5022 ,  C07F9/5045 ,  C07F9/5728 ,  C07F9/65515 ,  C07F9/65517 ,  C07F9/65522 ,  G01N33/531 ,  G01N33/553 ,  G01N33/587 ,  G01N33/6854 ,  G01N2333/916

Abstract: Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

Abstract translation: 本文公开了抗体 - 纳米颗粒缀合物，其包括直接连接到抗体或其片段的两个或更多个纳米颗粒（例如金，钯，铂，银，铜，镍，钴，铱或其两种或多种的合金） 金属硫醇键。 制备本文公开的抗体 - 纳米颗粒缀合物的方法包括使芳基膦 - 纳米颗粒复合物与还原抗体反应以产生抗体 - 纳米颗粒缀合物。 本文还公开了用于检测样品中靶分子的方法，其包括使用抗体 - 纳米颗粒缀合物（例如本文所述的抗体 - 纳米颗粒缀合物）和利用本文公开的方法检测靶分子的试剂盒。

公开(公告)号：US5691456A

公开(公告)日：1997-11-25

申请号：US444128

申请日：1995-05-18

Applicant: Maciej Adamczyk ,  Donald D. Johnson ,  Phillip G. Mattingly ,  Diana E. Clarisse ,  Joan D. Tyner ,  Mary M. Perkowitz

Inventor： Maciej Adamczyk ,  Donald D. Johnson ,  Phillip G. Mattingly ,  Diana E. Clarisse ,  Joan D. Tyner ,  Mary M. Perkowitz

IPC: C07D493/10 ,  C07F9/6561 ,  C07K16/18 ,  C07K16/44 ,  C09B11/08 ,  G01N33/532 ,  G01N33/533 ,  G01N33/78 ,  C07K17/00

CPC classification number: C07D493/10 ,  C07F9/6561 ,  C07K16/18 ,  C07K16/44 ,  C09B11/08 ,  G01N33/532 ,  G01N33/533 ,  G01N33/78 ,  Y10S436/80 ,  Y10S530/807

Abstract: Immunoassay methods and reagents for the specific quantification of thyroxine in a test sample are disclosed employing antibodies prepared with thyroxine derivatives of the formula: ##STR1## wherein P is an immunogenic carrier material and X is a linking moiety. The present invention also describes the synthesis of unique labelled reagent of the formula: ##STR2## wherein Q is a detectable moiety and W is a linking moiety, preferably fluorescein or a fluorescein derivative.

Abstract translation: 使用用下式的甲状腺素衍生物制备的抗体公开了用于测试样品中甲状腺素特异性定量的免疫测定方法和试剂：其中P是免疫原性载体材料，X是连接部分。 本发明还描述了具有下式的独特标记试剂的合成：其中Q是可检测部分，W是连接部分，优选荧光素或荧光素衍生物。

公开(公告)号：US5359093A

公开(公告)日：1994-10-25

申请号：US973140

申请日：1992-11-06

Applicant: Maciej Adamczyk ,  Donald D. Johnson ,  Phillip G. Mattingly ,  Diana E. Clarisse ,  Joan D. Tyner ,  Mary M. Perkowitz

Inventor： Maciej Adamczyk ,  Donald D. Johnson ,  Phillip G. Mattingly ,  Diana E. Clarisse ,  Joan D. Tyner ,  Mary M. Perkowitz

IPC: C07D493/10 ,  C07F9/6561 ,  C07K16/18 ,  C07K16/44 ,  C09B11/08 ,  G01N33/532 ,  G01N33/533 ,  G01N33/78

CPC classification number: C07D493/10 ,  C07F9/6561 ,  C07K16/18 ,  C07K16/44 ,  C09B11/08 ,  G01N33/532 ,  G01N33/533 ,  G01N33/78 ,  Y10S436/80 ,  Y10S530/807

Abstract: Immunoassay methods and reagents for the specific quantification of thyroxine in a test sample are disclosed employing antibodies prepared with thyroxine derivatives of the formula: ##STR1## wherein P is an immunogenic carrier material and X is a linking moiety. The present invention also describes the synthesis of unique labelled reagent of the formula: ##STR2## wherein Q is a detectable moiety and W is a linking moiety, preferably fluorescein or a fluorescein derivative.

Abstract translation: 使用用下式的甲状腺素衍生物制备的抗体公开了用于测试样品中甲状腺素特异性定量的免疫测定方法和试剂：其中P是免疫原性载体材料，X是连接部分。 本发明还描述了具有下式的独特标记试剂的合成：其中Q是可检测部分，W是连接部分，优选荧光素或荧光素衍生物。

公开(公告)号：US5354693A

公开(公告)日：1994-10-11

申请号：US83928

申请日：1993-06-29

Applicant: Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

Inventor： Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

IPC: G01N33/53 ,  A61K39/00 ,  G01N33/531 ,  G01N33/533 ,  G01N33/542 ,  G01N33/94

CPC classification number: G01N33/542 ,  G01N33/533 ,  G01N33/946 ,  Y10S436/816 ,  Y10S436/901 ,  Y10T436/13

Abstract: A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

Abstract translation: 提供了用于检测测试样品中一种或多种苯丙胺类分析物的存在的荧光偏振免疫测定（FPIA）。 免疫测定使用分子和荧光标记的示踪剂之间的竞争，用于苯乙胺衍生物特异性抗体上的结合位点。 样品中苯丙胺类分析物的浓度决定了与抗体结合的示踪剂的量。 可以定量测量形成的示踪剂 - 抗体复合物的量，并且与测试样品中分析物的量成反比。 本发明涉及示踪剂，用于引发用作测定试剂的抗体的免疫原，以及包含这些示踪剂和测定试剂的试剂盒。

公开(公告)号：US5248791A

公开(公告)日：1993-09-28

申请号：US820729

申请日：1992-01-14

Applicant: Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

Inventor： Paul J. Brynes ,  Donald D. Johnson ,  Cynthia M. Molina ,  Charles A. Flentge ,  Patrick F. Jonas

IPC: G01N33/533 ,  G01N33/542 ,  G01N33/94

CPC classification number: G01N33/533 ,  G01N33/542 ,  G01N33/946

Abstract: A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.

Abstract translation: 提供了用于检测测试样品中一种或多种苯丙胺类分析物的存在的荧光偏振免疫测定（FPIA）。 免疫测定使用分子和荧光标记的示踪剂之间的竞争，用于苯乙胺衍生物特异性抗体上的结合位点。 样品中苯丙胺类分析物的浓度决定了与抗体结合的示踪剂的量。 可以定量测量形成的示踪剂/抗体复合物的量，并且与测试样品中分析物的量成反比。 本发明涉及示踪剂，用于引发用作测定试剂的抗体的免疫原，以及包含这些示踪剂和测定试剂的试剂盒。

公开(公告)号：US5174969A

公开(公告)日：1992-12-29

申请号：US681150

申请日：1991-04-05

Applicant: Edward M. Fischer ,  Donald D. Johnson ,  Stephen M. Sanocki

Inventor： Edward M. Fischer ,  Donald D. Johnson ,  Stephen M. Sanocki

IPC: F01N3/02 ,  F01N3/021 ,  F01N3/022 ,  F02B3/06

CPC classification number: F01N3/0211 ,  F01N3/0226 ,  F01N2330/00 ,  F01N2330/08 ,  F02B3/06 ,  Y02T10/20 ,  Y10S55/10 ,  Y10S55/30

Abstract: An efficient, economical, compact diesel particulate filter comprising a casing radially filled with a bundle of tubes comprising woven, braided, or knitted inorganic yarn, wherein each tube is at least about twice the length of the bundle, and is folded at one end to prevent exhaust from traveling through the hollow of the tube without passing through its wall.

Abstract translation: 一种高效，经济，紧凑的柴油微粒过滤器，其包括径向填充有包括编织，编织或针织无机纱线的管束的壳体，其中每个管至少约束长度的两倍，并且在一端折叠 防止排气穿过管的中空而不穿过其壁。

公开(公告)号：US5115129A

公开(公告)日：1992-05-19

申请号：US662864

申请日：1991-03-01

Applicant: Donald D. Johnson

Inventor： Donald D. Johnson

IPC: G01J1/02 ,  G01J1/04 ,  G01V8/14 ,  H01L31/0232 ,  H01L33/00

CPC classification number: H01L31/0232 ,  G01J1/04 ,  G01J1/0411 ,  G01V8/14 ,  G01J1/0271 ,  G01J2001/028

Abstract: A photoelectric device is provided which incorporates a one piece housing having a first end which is light transmissive and liquid impermeable. An opening is provided at the other end of the tubular structure for insertion of photoelectric and electronic components into the cavity of the housing structure. A cover is used to seal an opening at the second end. The cover is ultrasonically welded to the second end of the tubular structure and a cable means is provided to permit electrical current to pass through the cover at the second end of the housing structure. The first end of the tubular structure, which is transmissive to visible, infrared or ultraviolet light can be formed into one or more lenses, depending on the application of the photoelectric device. Threads are provided in the outer cylindrical surface of the housing to aid in attaching the photoelectric device to brackets with the use of threaded nuts.

Abstract translation: 提供了一种光电装置，其包括具有透光性和液体不可渗透性的第一端的一体式壳体。 在管状结构的另一端设有开口，用于将光电和电子部件插入到壳体结构的空腔中。 盖用于密封第二端的开口。 盖被超声波焊接到管状结构的第二端，并且提供电缆装置以允许电流在壳体结构的第二端处通过盖。 取决于光电装置的应用，对可见光，红外线或紫外光透射的管状结构的第一端可以形成为一个或多个透镜。 螺纹设置在外壳的外圆柱表面，以帮助使用螺母将光电装置连接到支架上。