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公开(公告)号：US20200080047A1
公开(公告)日：2020-03-12
申请号：US16128121
申请日：2018-09-11
Applicant: General Electric Company
Inventor： Matthew Jeremiah Misner , Rachel Marie Gettings , Christine Angela Morton , Erik Leeming Kvam , Jason William Castle , Anindya Kanti De , Jared Timothy Hale , Craig Patrick Galligan , Vincent Francis Pizzi
Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.
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公开(公告)号：US20180361317A1
公开(公告)日：2018-12-20
申请号：US16012147
申请日：2018-06-19
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nicole Lea Wood , Li Zhu , Craig Patrick Galligan
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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公开(公告)号：US20180001231A1
公开(公告)日：2018-01-04
申请号：US15209411
申请日：2016-07-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossmann , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, JR. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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公开(公告)号：US20140190831A1
公开(公告)日：2014-07-10
申请号：US14099922
申请日：2013-12-07
Applicant: General Electric Company
IPC: F04B19/00
CPC classification number: F04B19/006 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2400/0418 , B01L2400/0605 , F16K99/0026 , F16K99/0042 , F16K99/0059 , Y10T137/0318 , Y10T436/2575
Abstract: A method of actuating a valve, comprises operatively coupling the valve with an electroosmotic pump; flowing a fluid through the electroosmotic pump; and generating a fluidic pressure of at least 0.75 PSI to actuate the valve, wherein the electroosmotic pump comprises one or more thin, porous, positive electroosmotic membranes and one or more thin porous, negative electroosmotic membranes; a plurality of electrodes comprising cathodes and anodes, and a power source; wherein each of the positive and negative electroosmotic membranes are disposed alternatively and wherein at least one of the cathodes is disposed on one side of one of the membranes and at least one of the anodes is disposed on the other side of the membrane and wherein at least one of the cathodes or anodes is disposed between a positive and a negative electroosmotic membrane.
Abstract translation: 一种致动阀门的方法包括将阀门与电渗泵可操作地连接; 使流体流过电渗泵; 并且产生至少0.75PSI的流体压力来致动所述阀，其中所述电渗泵包括一个或多个薄的，多孔的电渗透膜和一个或多个薄多孔的阴电渗膜; 包括阴极和阳极的多个电极和电源; 其中所述正电渗渗膜和所述阴电渗膜中的每一个交替设置，并且其中至少一个所述阴极设置在所述膜之一的一侧上，并且所述阳极中的至少一个设置在所述膜的另一侧上，并且其中至少 阴极或阳极之一设置在正电沉积膜和负电渗膜之间。
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公开(公告)号：US10787695B2
公开(公告)日：2020-09-29
申请号：US15611586
申请日：2017-06-01
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Christine Lynne Surrette , Erik Leeming Kvam , Steven Yuehin Go , Feng Chen , John Richard Nelson , Craig Patrick Galligan , Radislav Alexandrovich Potyrailo , Gregory Andrew Grossmann
IPC: C12Q1/02 , C12M1/34 , G01N21/64 , C12Q1/18 , G01N33/483
Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.
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Patent Agency Ranking
公开(公告)号：US10518196B2
公开(公告)日：2019-12-31
申请号：US15209411
申请日：2016-07-13
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossman , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, Jr. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
IPC: B01D21/00 , B01D21/24 , G01N33/49 , B01L3/00 , G01N1/40 , C12M1/12 , C12M1/26 , B01D17/02 , B01D21/02 , C12M1/00 , C02F1/48 , C02F1/44 , C02F101/32
Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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公开(公告)号：US09999856B2
公开(公告)日：2018-06-19
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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公开(公告)号：US20170363561A1
公开(公告)日：2017-12-21
申请号：US15532233
申请日：2015-12-03
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Andrea Jeannine Peters , Thomas Elliot Stecher , Craig Patrick Galligan , Christopher Michael Puleo , Ralf Lenigk
IPC: G01N27/30 , G01N27/453 , H01G11/86 , C12M1/00 , H01G11/02 , H01G9/22 , H01G9/042 , H01G11/28 , H01G11/48 , H01M4/60
CPC classification number: G01N27/30 , C12M47/06 , G01N27/453 , H01G9/042 , H01G9/22 , H01G11/02 , H01G11/28 , H01G11/48 , H01G11/86 , H01M4/602 , Y02E60/13
Abstract: The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.
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公开(公告)号：US09333463B2
公开(公告)日：2016-05-10
申请号：US13951929
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: G01N27/447 , B01D57/02
CPC classification number: B01D57/02 , G01N27/4473
Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.
Abstract translation: 提供了一种通过电洗脱从生物样品中洗脱生物分子的装置和系统。 用于从生物样品电洗脱生物分子的装置由构造成接收电解质和生物样品的壳体构成，至少两个电极包括可操作地耦合到壳体的导电氧化还原聚合物，以及生物分子不可渗透层，其设置在至少一个 电极。 生物分子不渗透层设置在至少一个电极上以防止生物分子到达电极。 提供了一种系统，其中系统包括样品采集端口，一个或多个包含缓冲液，溶剂，试剂或其组合的储存器，用于电洗脱的装置和控制器。
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20.发明申请公开(公告)号：US20150166956A1
公开(公告)日：2015-06-18
申请号：US14167393
申请日：2014-01-29
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , Erik Leeming Kvam , Gregory Andrew Grossmann , Craig Patrick Galligan , Jason Michael Nichols , Xuefeng Wang , Jason Louis Davis
IPC: C12N5/078
CPC classification number: B01D21/0087 , B01D17/02 , B01D21/0006 , B01D21/0042 , B01D21/02 , B01D21/10 , B01D21/2433 , B01D21/2444 , B01D21/245 , B01D2221/04 , B01L3/502753 , B01L3/502761 , B01L2200/0631 , B01L2200/0652 , B01L2200/0668 , B01L2300/0681 , B01L2300/0851 , B01L2400/0421 , B01L2400/043 , B01L2400/0457 , B01L2400/0469 , C02F1/00 , C02F1/24 , C02F1/444 , C02F1/48 , C02F2001/007 , C02F2101/32 , C02F2103/08 , C02F2103/10 , C02F2201/002 , C12M47/02 , C12N5/0641
Abstract: A device is configured for separation of particulates dispersed within a base fluid, wherein the particulates have a relative density difference compared to the base fluid. The device comprises a microchannel of length l and height h comprising an inlet and an outlet; a microporous surface on one or more walls of the microchannel; a collection chamber on an opposing side of the microporous surface; and an applied force field across the height h of the microchannel to sediment the particles through the microporous surface into the collection chamber. The microporous body operationally generates a fluid flow regime comprising a first fluid flow having a first flow rate through the microchannel and a second fluid flow having a second flow rate through the collection chamber and the second flow rate is a fraction of the first flow rate.
Abstract translation: 一种装置被配置用于分离分散在基础流体中的微粒，其中所述颗粒与基础流体相比具有相对密度差。 该装置包括长度为l的微通道和包括入口和出口的高度h; 在微通道的一个或多个壁上的微孔表面; 在微孔表面的相对侧上的收集室; 以及穿过微通道的高度h的施加的力场，以将颗粒通过微孔表面沉淀到收集室中。 微孔体可操作地产生流体流动状态，其包括具有通过微通道的第一流量的第一流体流和具有通过收集室的第二流速的第二流体流，第二流量是第一流量的一部分。
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