公开(公告)号：US10048483B2

公开(公告)日：2018-08-14

申请号：US15198634

申请日：2016-06-30

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Tomonori Nakamura ,  Ikuo Arata ,  Yoshihiro Ito

IPC: G02B21/36 ,  G02B5/00 ,  G02B21/00 ,  G02B21/04 ,  G02B21/16 ,  G02B21/24 ,  G02B27/00

Abstract: An optical device for microscopic observation 4 comprises: a cold stop 13 having openings 13d, 13e corresponding to a low-magnification microscope optical system 5 and being a stop member arranged in a vacuum vessel 12 to let the light from the sample S pass to the camera 3; a warm stop 10 having an opening 14 corresponding to a high-magnification microscope optical system 5 and being a stop member arranged outside the vacuum vessel 12 to let the light from the sample S pass toward the cold stop 13; and a support member 11 supporting the warm stop 10 so that the warm stop can be inserted to or removed from on the optical axis of the light from the sample S, wherein the warm stop 10 has a reflective surface 15 on the camera 3 side and wherein the opening 14 is smaller than the openings 13d, 13e.

公开(公告)号：US09435734B2

公开(公告)日：2016-09-06

申请号：US14806999

申请日：2015-07-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Norikazu Sugiyama ,  Takuji Kataoka ,  Ikuo Arata

IPC: G01N21/01 ,  G01N21/47 ,  G01N33/483 ,  G02B21/08 ,  G01N21/49 ,  G01N33/50 ,  G01N21/59 ,  G02B21/36

CPC classification number: G01N21/47 ,  G01N21/49 ,  G01N21/59 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2201/0446 ,  G01N2201/062 ,  G02B21/088 ,  G02B21/36

Abstract: A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11, mounting the petri dish 11 on a waveguide 21 via water 13, emitting illumination light L1 into the waveguide 21 and emitting the illumination light L1 to the stem cells C in the petri dish 11 via the water 13, and detecting scattered light L2, the scattered light L2 being the illumination light L1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21. Then, in the light image detected by means of the scattered light L2, a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.