公开(公告)号：US20230249176A1

公开(公告)日：2023-08-10

申请号：US18003284

申请日：2022-02-18

Applicant: Illumina, Inc.

Inventor： Jonathan Ziebarth ,  Jon Aday ,  Paul Crivelli ,  Gerald Kreindl ,  Amit Sharma

IPC: B01L3/00 ,  B29C45/14

CPC classification number: B01L3/502707 ,  B29C45/14778 ,  B01L2300/0896 ,  B29C2045/0094

Abstract: Provided herein include various examples of a method for manufacturing aspects of flow cell. The method may include performing chemical processes on a surface of the patterned wafer to prepare the surface of the patterned, singulating the wafer into individual dies, orienting each die on a temporary substrate, where the orienting creates spaces between each individual die, and molding a material over the spaces to create a hybrid wafer comprised of glass and molded material. The method may also include bonding two of the hybrid wafers together, forming a bonded wafer stack.

公开(公告)号：US11565252B2

公开(公告)日：2023-01-31

申请号：US16706332

申请日：2019-12-06

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  C12Q1/6837 ,  B01J19/00 ,  B01L9/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US11511274B2

公开(公告)日：2022-11-29

申请号：US16641092

申请日：2019-11-14

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Cyril Delattre

IPC: B01L3/00 ,  G01N35/10

Abstract: An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

公开(公告)号：US20210154667A1

公开(公告)日：2021-05-27

申请号：US17165757

申请日：2021-02-02

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

IPC: B01L3/00 ,  G01N1/31

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US20250050334A1

公开(公告)日：2025-02-13

申请号：US18929140

申请日：2024-10-28

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  B01J19/00 ,  B01L9/00 ,  C12Q1/6837

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US20240165627A1

公开(公告)日：2024-05-23

申请号：US18281018

申请日：2022-03-17

Applicant: ILLUMINA, INC.

Inventor： Mikko Ponkala ,  Paul Limb ,  Reto Schoch ,  Sonny Pham ,  Maulik Patel ,  Paul Crivelli

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0638

Abstract: Non-contact dispenser assemblies and related systems and methods are disclosed. An implementation of an apparatus is disclosed that includes a reagent cartridge including a body defining a plurality of reservoirs. Each reservoir has an outlet and a distal end defining an opening, a manifold assembly including an outlet, a common fluidic line fluidly coupled to the outlet, a plurality of reagent fluidic lines coupled to the corresponding outlet of the reservoirs, and a plurality of membrane valves selectively fluidly coupling the common fluidic line and a corresponding one of the plurality of reagent fluidic lines.

公开(公告)号：US20230226552A1

公开(公告)日：2023-07-20

申请号：US18009545

申请日：2021-05-24

Applicant: ILLUMINA, INC.

Inventor： Travis Beckman ,  Paul Crivelli ,  Wesley Cox-Muranami ,  Jennifer Foley ,  Darren Segale ,  Allison Duchnak ,  Keith Frankie ,  Cameron Mar

IPC: B01L3/00

CPC classification number: B01L3/523 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2200/025

Abstract: Dry reagent cup assemblies and methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir and dry reagent cup assembly. The liquid reservoir has a base, side wall that extends from the base, and distal opening. The dry reagent cup assembly coupled to the liquid reservoir includes a dry reagent cup and liquid impermeable barrier. The dry reagent cup has a cup base, cup side wall that extends from the cup base, and cup opening. The distal opening of the liquid reservoir faces the cup opening. The liquid impermeable barrier covers the cup opening and separates the liquid reservoir and the dry reagent cup. The dry reagent cup moves between an initial position outside the liquid reservoir and a rehydrating position where the dry reagent cup pierces and passes through an opening in the liquid impermeable barrier and is received within the liquid reservoir.

公开(公告)号：US20230184800A1

公开(公告)日：2023-06-15

申请号：US18075753

申请日：2022-12-06

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Justin Davidson ,  Norman Khoo

IPC: G01N35/10

CPC classification number: G01N35/1002 ,  G01N2035/00306

Abstract: Reagent reservoirs and related systems and methods are disclosed. In accordance with a first implementation, an apparatus includes a system and a reagent reservoir. The system includes a reagent reservoir receptacle. The reagent reservoir is received within the reagent reservoir receptacle and has a body and a fluidic port. The body defines a storage chamber, a sipper chamber, and a fluidic sinus fluidly coupling the storage chamber and the sipper chamber. The fluidic port is fluidly coupled to the sipper chamber.

公开(公告)号：US20230158493A1

公开(公告)日：2023-05-25

申请号：US18154563

申请日：2023-01-13

Applicant: Illumina, Inc.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  C12Q1/6837

CPC classification number: B01L3/502715 ,  C12Q1/6837 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2400/0655 ,  B01L2300/0887 ,  B01L2200/16 ,  B01J19/0046

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US11372016B2

公开(公告)日：2022-06-28

申请号：US16584306

申请日：2019-09-26

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  James Osmus ,  Paul Crivelli ,  Bradley Kent Drews

IPC: G01N35/10

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.