公开(公告)号：US06529271B1

公开(公告)日：2003-03-04

申请号：US09467801

申请日：1999-12-20

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G01N2164

CPC classification number: G06T7/00 ,  G01N27/44721 ,  G02B21/0076 ,  G02B21/008

Abstract: A method of finding, recording and optionally evaluating object structures, especially on slides, preferably of fluorescent object structures such as gene spots. A microscope with a CCD camera, a scanning microscope or a preferably confocal laser scanning microscope can be used for recording and for the rapid and reliable detection of the object structures, with the image data being recorded using an illumination pattern that is projected into the object plane.

Abstract translation: 发现，记录和任选地评估对象结构的方法，特别是在载玻片上，优选荧光物体结构例如基因点。 具有CCD照相机，扫描显微镜或优选共聚焦激光扫描显微镜的显微镜可用于记录和用于物体结构的快速和可靠的检测，其中使用投射到物体中的照射图案来记录图像数据 飞机

公开(公告)号：US06423960B1

公开(公告)日：2002-07-23

申请号：US09476589

申请日：1999-12-31

Applicant: Johann Engelhardt ,  Bernd Widzgowski

Inventor： Johann Engelhardt ,  Bernd Widzgowski

IPC: H01J4014

CPC classification number: G02B21/0084 ,  G02B21/004 ,  G02B21/0048

Abstract: The invention discloses a method and system for processing scan signals from a confocal microscope. The confocal microscope comprises an illumination source and a scanning device with a scanning mirror system. A control and processing unit is provided, which unit uses a plurality of programmable devices for the real time processing of digital signals. The control and processing unit has at least three input ports and one output port. A first detector generates analog signals corresponding to the light reflected from a specimen within the microscope and a second detector generates analog signals corresponding to the intensity of the light from the illumination source. In addition, a position signal of the scanning laser beam is provided to the control and processing unit. Analog-to-digital converters receive the analog signals, generate digital signals and provide the digital signals to the input ports of the control and processing unit.

Abstract translation: 本发明公开了一种从共焦显微镜处理扫描信号的方法和系统。 共焦显微镜包括照明源和具有扫描镜系统的扫描装置。 提供了一种控制和处理单元，该单元使用多个可编程设备来进行数字信号的实时处理。 控制和处理单元至少有三个输入端口和一个输出端口。 第一检测器产生对应于在显微镜内从样本反射的光的模拟信号，第二检测器产生对应于来自照明源的光的强度的模拟信号。 此外，扫描激光束的位置信号被提供给控制和处理单元。 模数转换器接收模拟信号，产生数字信号并将数字信号提供给控制和处理单元的输入端口。

公开(公告)号：US06266148B1

公开(公告)日：2001-07-24

申请号：US09147976

申请日：1999-05-17

Applicant: Johann Engelhardt ,  Thomas Zapf

Inventor： Johann Engelhardt ,  Thomas Zapf

IPC: G01B1124

CPC classification number: A61B1/00183 ,  A61B5/0068 ,  A61B5/0088 ,  A61C9/0053 ,  A61C19/04 ,  G01B11/24

Abstract: The invention relates to a method for measuring surfaces by confocal microscopy using the reflection method, specially to measure the superficial profiles (1) of treated or drilled teeth (2). The invention seeks to eliminate mistakes occurring when very inclined areas are measured. To this end, the method disclosed is characterized by a confocal representation with enhanced dynamics (relative sensitivity) enabling it to project both the retro-reflections and the weak scattered light (3) or fluorescent light of each focal plane (8).

Abstract translation: 本发明涉及一种通过共聚焦显微镜使用反射法测量表面的方法，特别是用于测量经处理或钻孔的牙齿（1）的表面轮廓（1）。 本发明试图消除在测量非常倾斜的区域时发生的错误。 为此，所公开的方法的特征在于具有增强的动力学（相对灵敏度）的共聚焦表征，使其能够投射每个焦平面（8）的反射和弱散射光（3）或荧光。

公开(公告)号：US06263234B1

公开(公告)日：2001-07-17

申请号：US09147995

申请日：1999-03-24

Applicant: Johann Engelhardt ,  Thomas Zapf

Inventor： Johann Engelhardt ,  Thomas Zapf

IPC: A61B600

CPC classification number: A61B5/0088 ,  A61B5/0068 ,  A61C9/0053 ,  A61C13/0004

Abstract: The invention concerns a device for the confocal measuring of surfaces inside cavities of the body, specially to measure the surface profile (1) of teeth (2) in the mouth cavity. Said device has a probe (3) that can be introduced into the cavity of the body, a light source feeding the probe (3), a detector picking up a light signal (5) and a processor (6) to digitalize the detected signal transforming it into a tridimensional representation. The device is designed using a simple construction and enabling an error free scanning of the surfaces. To this end, the probe (3) is designed as a rotary scanner having at least one deviating device (7) deflecting the light beam (9) in the direction of the surface that is to be measured (1), the deviating device (7) can be positioned in another scanning axis (10) to forward the rotating light beam (9), and the detector (5) comprises a device for sequential or simultaneous scanning of several focal planes, both with regards to specular reflection and to weak scattered light or fluorescent light of the focal plane concerned.

Abstract translation: 本发明涉及一种用于共焦测量身体内部表面的表面的装置，特别是用于测量口腔中牙齿（2）的表面轮廓（1）。 所述装置具有可以被引入到身体的空腔中的探针（3），馈送探针（3）的光源，拾取光信号的检测器（5）和处理器（6）以将检测到的信号数字化 将其转变为三维表征。 该设备采用简单的结构设计，可以对表面进行无错误的扫描。 为此，探针（3）被设计为具有至少一个偏转装置（7）的旋转扫描仪，偏转装置（7）沿着待测表面（1）的方向偏转光束（9），偏移装置 7）可以定位在另一个扫描轴（10）中，以转动旋转光束（9），并且检测器（5）包括用于连续或同时扫描几个焦平面的装置，无论是针对镜面反射还是弱 焦平面的散射光或荧光。

公开(公告)号：US5903688A

公开(公告)日：1999-05-11

申请号：US793005

申请日：1997-02-06

Applicant: Johann Engelhardt ,  Heinrich Ulrich ,  Klaus Kierschke

Inventor： Johann Engelhardt ,  Heinrich Ulrich ,  Klaus Kierschke

IPC: G02B21/00 ,  G02B21/16 ,  G02B6/42

CPC classification number: G02B21/0024 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/16

Abstract: A device for feeding a light beam from a UV laser (1) into a laser scanning microscope (2) has a beam-alignment assembly (3) for aligning the laser beam with the beam path of the microscope (2) and an optical-fiber element (4) located between the UV laser (1) and the beam-alignment assembly (3). The aim of the invention is to design the feed device so that it can be used for long periods in order to keep the time and expense necessary to change the feed device as low as possible. To achieve this, the invention provides a beam interrupter (5) located between the UV laser (1) and the optical-fiber element (4). The beam interrupter allows the beam from the UV laser (1) to pass through the optical-fiber element (4) only during scanning, i.e., when pictures are being generated.

Abstract translation: PCT No.PCT / EP95 / 02627 Sec。 371日期1997年2月6日 102（e）日期1997年2月6日PCT提交1995年7月6日PCT公布。 出版物WO96 / 06377 日期：1996年2月29日用于将来自UV激光器（1）的光束馈送到激光扫描显微镜（2）的装置具有用于将激光束与显微镜（2）的光束路径对准的光束对准组件（3） ）和位于UV激光（1）和光束对准组件（3）之间的光纤元件（4）。 本发明的目的是设计进给装置，使得其可以长时间使用，以便保持尽可能低地改变进给装置所需的时间和费用。 为了实现这一点，本发明提供一种位于UV激光器（1）和光纤元件（4）之间的光束断续器（5）。 光束断续器仅允许来自UV激光器（1）的光束仅在扫描期间即当图像被产生时通过光纤元件（4）。

公开(公告)号：US07274506B2

公开(公告)日：2007-09-25

申请号：US11327783

申请日：2006-01-06

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B21/18

CPC classification number: G02B21/241 ,  G02B21/18

Abstract: In adjusting a microscope having at least two objectives for superimposing measuring beams of light in an object space to the end of obtaining an interference pattern and for monitoring the object space, each objective having a focal point, a focal plane and a pupil, auxiliary beams of light which are distinguishable from the measuring beams are directed into the objectives, and the auxiliary beams getting back out of the objectives are superimposed to obtain an auxiliary interference pattern. Further, the auxiliary beams getting back out of the objectives are imaged as spots.

Abstract translation: 在调整具有至少两个物镜的显微镜的情况下，将物镜空间中的测量光束叠加到获得干涉图案的末端并用于监视物体空间，每个物镜具有焦点，焦平面和瞳孔，辅助光束 与测量光束区分开的光被引导到物镜中，并且重叠回目标的辅助光束被叠加以获得辅助干涉图案。 此外，从目标回退的辅助光束成像为点。

公开(公告)号：US06900935B2

公开(公告)日：2005-05-31

申请号：US09906435

申请日：2001-07-16

Applicant: Johann Engelhardt ,  Juergen Hoffmann

Inventor： Johann Engelhardt ,  Juergen Hoffmann

IPC: G02B21/06 ,  G02B21/00 ,  G02B21/08 ,  G02B27/28

CPC classification number: G02B27/28 ,  G02B21/002 ,  G02B21/082

Abstract: An optical arrangement, in particular a microscope, having a light source (1) for illuminating an object, and a glass fiber (3), arranged between the light source (1) and the object, for transporting light along a prescribable distance between the light source (1) and the object, is configured with regard to the avoidance of fluctuations in the illuminating light power without a handicap in the optical adjustment of the arrangement in such a way that the glass fiber (3) is a polarizing glass fiber (3).

Abstract translation: 具有用于照射物体的光源（1）和布置在光源（1）和物体之间的玻璃纤维（3））的光学装置，特别是显微镜，用于沿着光源 光源（1）和物体被配置为在玻璃纤维（3）是偏光玻璃纤维（3）的方式进行光学调整时避免照明光功率波动而没有障碍， 3）。

公开(公告)号：US06852967B2

公开(公告)日：2005-02-08

申请号：US10188622

申请日：2002-07-03

Applicant: Johann Engelhardt ,  Werner Knebel

Inventor： Johann Engelhardt ,  Werner Knebel

IPC: G02B21/00 ,  G01G3/50

CPC classification number: G02B21/0064

Abstract: A scanning microscope for examination of a sample (31), having at least one optical component (89) that exhibits a wavelength-dependent characteristic and having an apparatus for wavelength-dependent detection that acquires measured values in at least two wavelength regions each characterized by a spectral width and a spectral position, is disclosed. The scanning microscope is characterized in that the wavelength-dependent characteristic of the at least one optical component (89) can be ascertained, can be at least temporarily stored in the form of a data set in a memory (49, 81), and can be considered upon acquisition and/or upon utilization of the measured values.

Abstract translation: 一种用于检查样品（31）的扫描显微镜，其具有至少一个具有波长相关特性的光学部件（89），并具有用于波长相关检测的装置，其获取至少两个波长区域中的测量值，每个特征在于 光谱宽度和光谱位置。 扫描显微镜的特征在于，可以确定至少一个光学部件（89）的波长相关特性，至少可以以数据集的形式临时存储在存储器（49,81）中，并且可以 在获得和/或利用测量值时考虑。

公开(公告)号：US06785302B1

公开(公告)日：2004-08-31

申请号：US09554083

申请日：2000-05-12

Applicant: Johann Engelhardt ,  Heinrich Ulrich ,  William C. Hay

Inventor： Johann Engelhardt ,  Heinrich Ulrich ,  William C. Hay

IPC: H01S3121

CPC classification number: G01N21/6458 ,  G02B21/0024 ,  G02B21/0076 ,  G02B21/008

Abstract: The invention relates to an optical system in the ray path of a confocal fluorescence microscope, comprising at least one laser light source (1, 2), a device positioned in the illuminating/detecting beam (3, 4, 5) for separating the exciting light (8) from the fluorescent light (9), an objective (10) arranged between the device and the object (7), and a detector (11) positioned downstream of the device situated in the detecting beam (5). The aim of the invention is to increase the fluorescence yield of the system while retaining its compact structure. To this end the separating device comprises a mirror (13) which is dimensioned and positioned in the illuminating and/or detecting beam (3, 4, 5) in such a way that for the dark-field illumination of the object (7) it reflects the non-expanded exciting beam arriving from the laser light source (1, 2) into the objective (10) and permits the transit of the fluorescence light (9) arriving from the object (7) in the direction of the detector (11) with full numerical aperture, the fluorescent light beam being reduced by the mirror (13) cross-section active in the detecting beam (5).

Abstract translation: 本发明涉及一种共聚焦荧光显微镜的光线路径中的光学系统，包括至少一个激光光源（1,2），位于照明/检测光束（3,4,5）中的装置，用于分离激发光 来自荧光灯（9）的光（8），布置在装置和物体（7）之间的物镜（10）和位于位于检测光束（5）中的装置下游的检测器（11）。 本发明的目的是提高系统的荧光收率，同时保持其紧凑的结构。 为此，分离装置包括反射镜（13），该反射镜的尺寸设置在照明和/或检测光束（3,4,5）中，使得对于物体（7）的暗场照明 反射从激光光源（1,2）到达物镜（10）的未扩展的激发光束，并允许从物体（7）到达检测器（11）的方向的荧光（9）的转移 ）具有全数值孔径，荧光光束被检测光束（5）中的反射镜（13）横截面减小。

公开(公告)号：US06754003B2

公开(公告)日：2004-06-22

申请号：US10218314

申请日：2002-08-14

Applicant: Johann Engelhardt

Inventor： Johann Engelhardt

IPC: G02B2106

CPC classification number: G02B21/0048 ,  G02B21/0052 ,  G02B21/0076

Abstract: A scanning microscope possesses at least one illumination source for emitting an illuminating beam that is conveyed via a beam deflection device and an optical system to a specimen and scans the latter, the beam deflection device defining at least one illuminating beam rotation point. A device for axial displacement in particular of the beam deflection device, or of a lens preceding the objective, is provided for imaging of an image of the illuminating beam rotation point into the pupil of the objective.

Abstract translation: 扫描显微镜具有至少一个照明源，用于发射通过光束偏转装置和光学系统传送到样本并照射后者的照明光束，所述光束偏转装置限定至少一个照射光束旋转点。 提供用于轴向位移的装置，特别是用于光束偏转装置或物镜之前的透镜的轴向位移，用于将照射光束旋转点的图像成像到物镜的瞳孔中。